Combination of multiphoton and reflective confocal imaging of cornea

Microscopy Research and Technique

Volumn 71, Issue 2, 2008, Pages 83-85

  Chen, Wei Liang ^a   Sun, Yen ^a   Lo, Wen ^a   Tan, Hsin Yuan ^b   Dong, Chen Yuan ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b CHANG GUNG MEMORIAL HOSPITAL   (Taiwan)

Author keywords

Confocal microscopy; Multiharmonic generation; Multiphoton processes; Ophthalmology; Three dimensional microscopy

Indexed keywords

CONFOCAL MICROSCOPY; CYTOLOGY; MAMMALS; NONLINEAR OPTICS;

AUTOFLUORESCENCE; CONFOCAL IMAGING; EPITHELIAL CELLS; IMAGING MODALITY; MINIMALLY-INVASIVE TECHNIQUE; MULTIHARMONIC; MULTIHARMONIC GENERATION; MULTIPHOTON MICROSCOPY; MULTIPHOTONS; THREE-DIMENSIONAL MICROSCOPY;

CELLS;

COLLAGEN;

ANIMAL TISSUE; ARTICLE; AUTOFLUORESCENCE; CELL ULTRASTRUCTURE; CONFOCAL MICROSCOPY; CORNEA CELL; CORNEA STROMA; COW; EPITHELIUM CELL; IMAGING SYSTEM; MINIMALLY INVASIVE SURGERY; MULTIPHOTON MICROSCOPY; NONHUMAN; PRIORITY JOURNAL;

BOVINAE;

EID: 39449106610     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20528     Document Type: Article

References (16)

1. Bohnke M, Masters BR. 1999. Confocal microscopy of the cornea. Prog Retin Eye Res 18:553-628.
2. Campagnola PJ, Loew LM. 2003. Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms. Nat Biotechnol 21:1356-1360.
3. Condeelis J, Segall JE. 2003. Intravital imaging of cell movement in tumours. Nat Rev Cancer 3:921-930.
4. Denk W, Strickler JH, Webb WW. 1990. 2-Photon laser scanning fluorescence microscopy. Science 248:73-76.
5. Levene MJ, Dombeck DA, Kasischke KA, Molloy RP, Webb WW. 2004. In vivo multiphoton microscopy of deep brain tissue. J Neurophysiol 91:1908-1912.
6. Masters BR, Bohnke M. 2001. Three-dimensional confocal microscopy of the human cornea in vivo. Ophthal Res 33:125-135.
7. Masters BR, Bohnke M. 2002. Three-dimensional confocal microscopy of the living human eye. Annu Rev Biomed Eng 4:69-91.
8. Piston DW, Masters BR, Webb WW. 1995. 3-Dimensionally resolved Nad(P)H cellular metabolic redox imaging of the in-situ cornea with 2-photon excitation laser-scanning microscopy. J MicroscOxford 178:20-27.
9. So PTC, Dong CY, Masters BR, Berland KM. 2000. Two-photon excitation fluorescence microscopy. Annu Rev Biomed Eng 2:399-429.
10. Tan HY, Teng SW, Lo W, Lin WC, Lin SJ, Jee SH, Dong CY. 2005. Characterizing the thermally induced structural changes to intact porcine eye, Part 1: Second harmonic generation imaging of cornea stroma. J Biomed Optics 10:054019.
11. Tan HY, Sun Y, Lo W, Lin SJ, Hsiao CH, Chen YF, Huang SCM, Lin WC, Jee SH, Yu HS, Dong CY. 2006. Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo. Invest Ophthalmol Vis Sci 47:5251-5259.
12. Teng SW, Tan HY, Peng JL, Lin HH, Kim KH, Lo W, Sun Y, Lin WC, Lin SJ, Jee SH, So PTC, Dong CY. 2006. Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye. Invest Ophthalmol Vis Sci 47:1216-1224.
13. Yeh AT, Nassif N, Zoumi A, Tromberg BJ. 2002. Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence. Opt Lett 27:2082-2084.
14. Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT, Webb WW. 2003a. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci USA 100:7075-7080.
15. Zipfel WR, Williams RM, Webb WW. 2003b. Nonlinear magic: Multiphoton microscopy in the biosciences. Nat Biotechnol 21:1369-1377.
16. Zoumi A, Yeh A, Tromberg BJ. 2002. Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence. Proc Natl Acad Sci USA 99:11014-11019.