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PLL and PMOXA, because of their structural similarity, differ only in some peak component concentration ratios but do not exhibit characteristic peaks that would be unique for only one of them. Therefore, the contribution of the PLL backbone has been neglected in the calculations. The error introduced by this assumption is comparatively small, given that the PLL backbone for the used architecture accounts for only a small atomic percentage (13.9 % N and C and 7.5 % O). Furthermore, contributions stemming from contamination or surfacehydroxyl/water were also neglected.
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PLL and PMOXA, because of their structural similarity, differ only in some peak component concentration ratios but do not exhibit characteristic peaks that would be unique for only one of them. Therefore, the contribution of the PLL backbone has been neglected in the calculations. The error introduced by this assumption is comparatively small, given that the PLL backbone for the used architecture accounts for only a small atomic percentage (13.9 % N and C and 7.5 % O). Furthermore, contributions stemming from contamination or surfacehydroxyl/water were also neglected.
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At very high, biologically irrelevant salt concentrations, both PLL-g-PEG and PLL-g-PMOXA desorb from the surface because of strong screening of the electrostatic interaction. Recently, PLL-g-PEG was covalently linked to an aldehyde plasma polymer to overcome this limitation. In this case, the layer was stable and resistant to protein adsorption up to very high ionic strengths (2.4 mol/L NaCl).31
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