Isolation and characterization of the CD133 + precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting

Biotechnology Letters

Volumn 26, Issue 14, 2004, Pages 1131-1136

  Yu, S ^a   Zhang, J Z ^a   Zhao, C L ^a   Zhang, H Y ^a   Xu, Q ^a  

^a CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

CD133; magnetic affinity cell sorting; neural precursors; stem cells

Indexed keywords

CD133 ANTIGEN; GLYCOPROTEIN; LEUKOCYTE ANTIGEN; PEPTIDE;

ARTICLE; BIOSYNTHESIS; BRAIN; CELL DIFFERENTIATION; CELL LINEAGE; CELL PROLIFERATION; CELL SEPARATION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; IMMUNOHISTOCHEMISTRY; ISOLATION AND PURIFICATION; MAGNETISM; METABOLISM; METHODOLOGY; NERVE CELL; PRENATAL DEVELOPMENT; TIME;

ANTIGENS, CD; BRAIN; CELL DIFFERENTIATION; CELL LINEAGE; CELL PROLIFERATION; CELL SEPARATION; FLOW CYTOMETRY; GLYCOPROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; MAGNETICS; MICROSCOPY, FLUORESCENCE; NEURONS; PEPTIDES; TIME FACTORS;

EID: 3943050097     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000035484.64499.ac     Document Type: Article

References (9)

1. + cells on a magnetic cell separator (MACS). J. Immunol. Meth. 186: 233-243.
2. Miltenyi S, Muller W, Weichel W, Radbruch A (1990) High gradient magnetic cells eparation with MACS. Cytometry 11: 231-238.
3. Peichev M, Naiyer AJ, Pereira D, Zhu Z, Lane WJ, Williams M, Oz MC, Hicklin DJ, Witte L, Moore MA, Rafii S (2000) Expression of VEGFR-2 and AC133 by circulating human CD34 (+) cells identifies a population of functional endothelial precursors. Blood 95: 952-958.
4. Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255: 1707-1710.
5. Suslov ON, Kukekov VG, Ignatova TN, Steindler DA (2002) Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres. Proc. Natl. Acad. Sci. USA 99: 14506-14511.
6. Svendsen CN, ter Borg MG, Armstrong RJ, Rosser AE, Chandran S, Ostenfeld T, Caldwell MA (1998) A new method for the rapid and long term growth of human neural precursor cells. J. Neurosci. Meth. 85: 141-152.
7. Tamaki S, Eckert K, He D, Sutton R, Doshe M, Jain G, Tushinski R, Reitsma M, Harris B, Tsukamoto A, Gage F, Weissman I, Uchida N (2002) Engraftment of sorted/expanded human central nervous system stem cells from fetal brain. J. Neurosci. Res. 69: 976-986.
8. Uchida N, Buck DW, He D, Reitsma MJ, Masek M, Phan TV, Tsukamoto AS, Gage FH, Weissman IL (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. USA 97: 14720-14725.
9. Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012.