Increased efficiency of genetic profiling through quantity and quality assessment of fluorescently labeled oligonucleotide primers

BioTechniques

Volumn 44, Issue 1, 2008, Pages 49-52

  Frasier, Timothy R ^a   White, Bradley N ^a  

^a TRENT UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE;

ARTICLE; DEVICE; DNA DETERMINATION; DNA FINGERPRINTING; ELECTROPHORESIS; FLUORESCENCE; GENETIC ANALYSIS; GENOTYPE; POLYMERASE CHAIN REACTION; QUALITY CONTROL; QUANTITATIVE ANALYSIS;

DNA PRIMERS; FLUORESCENT DYES; GENE EXPRESSION PROFILING; QUALITY CONTROL; REFERENCE STANDARDS;

EID: 39149103427     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112678     Document Type: Article

References (10)

1. Schlötterer, C. 2004. The evolution of molecular markers - just a matter of fashion? Nat. Rev. Genet. 5:63-69.
2. Chamberlain, J.S., R.A. Gibbs, J.E. Ranier, P.N. Nguyen, and C.T. Caskey. 1988. Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res. 16:11141-11156.
3. Smith, L.M., S. Fung, M.W. Hunkapiller, T.J. Hunkapiller, and L.E. Hood. 1985. The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis. Nucleic Acids Res. 13:2399-2412.
4. Wallin, J.M., C.L. Holt, K.D. Lazaruk, T.H. Nguyen, and P.S. Walsh. 2002. Constructing universal multiplex PCR systems for comparative genotyping. J. Forensic Sci. 47:52-65.
5. Frasier, T.R., T. Rastogi, M.W. Brown, P.K. Hamilton, S.D. Kraus, and B.N. White. 2006. Characterization of tetranucleotide microsatellite loci and development and validation of multiplex reactions for the study of right whale species (genus Eubalaena). Mol. Ecol. Notes 6:1025-1029.
6. Cullingham, C.I., C.J. Kyle, and B.N. White. 2006. Isolation, characterization and multiplex genotyping of raccoon tetranucleotide microsatellite loci. Mol. Ecol. Notes 6:1030-1032.
7. Henegariu, O., N.A. Heerema, S.R. Dlouhy, G.H. Vance, and P.H. Vogt. 1997. Multiplex PCR: critical parameters and step-by-step protocol. BioTechniques 23:504-511.
8. Markoulatos, P., N. Siafakas, and M. Moncany. 2002. Multiplex polymerase chain reaction: a practical approach. J. Clin. Lab. Anal. 16:47-51.
9. Butler, J.M., J.M. Devaney, M.A. Marino, and P.M. Vallone. 2001. Quality control of PCR primers used in multiplex STR amplification reactions. Forensic Sci. Int. 119:87-96.
10. Applied Biosystems. 1997. GeneScan® Reference Guide: Chemistry Reference for the ABI PRISM™ 377 and ABI™ 373 DNA Sequencers. The Perkin-Elmer Corporation, Applied Biosystems Division, Foster City, CA.