Silica-guanidinium thiocyanate-based nucleic acid isolation protocol does not improve sensitivity of two commercial tests for detection of Mycobacterium tuberculosis in respiratory samples

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 25, Issue 10, 2006, Pages 673-675

  van Doorn, H R ^a   Bruijnesteijn van Coppenraet, E S ^b   Duim, B ^a   Vandenbroucke Grauls, C M J E ^a,c   Weel, J F ^d   Dankert, J ^a   Kuijper, E J ^b   de Jong, M D ^e  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^d Public Health Service of Northern Friesland   (Netherlands)

^e OXFORD UNIVERSITY CLINICAL RESEARCH UNIT   (Viet Nam)

Author keywords

Pleural Fluid; Respiratory Sample; Sputum Sample; Tuberculosis; Tuberculosis Complex

Indexed keywords

GUANIDINE DERIVATIVE; GUANIDINE THIOCYANATE; NUCLEIC ACID; SILICON DIOXIDE; THIOCYANIC ACID DERIVATIVE;

ARTICLE; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; LUNG LAVAGE; MICROBIOLOGY; MYCOBACTERIUM TUBERCULOSIS; SENSITIVITY AND SPECIFICITY; SPUTUM; TUBERCULOSIS;

BRONCHOALVEOLAR LAVAGE FLUID; GUANIDINES; HUMANS; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACIDS; SENSITIVITY AND SPECIFICITY; SILICON DIOXIDE; SPUTUM; THIOCYANATES; TUBERCULOSIS;

EID: 39049196315     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-006-0194-z     Document Type: Article

References (8)

1. Drobniewski FA, Caws M, Gibson A, Young D (2003) Modern laboratory diagnosis of tuberculosis. Lancet Infect Dis 3:141–147 DOI: 10.1016/S1473-3099(03)00544-9
2. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J (1990) Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28:495–503
3. Petroff SA (1915) A new and rapid method for the isolation and cultivation of tubercle bacilli directly from the sputum and feces. J Exp Med 21:38–42 DOI: 10.1084/jem.21.1.38
4. Kubica GP, Dye WE, Cohn ML, Middlebrook G (1963) Sputum digestion and decontamination with N-acetyl-l-cysteine-sodium hydroxide for culture of mycobacteria. Am Rev Respir Dis 87:775–779
5. Mulder S, Noordhoek GT (2001) Sensitivity of COBAS Amplicor system for detection of Mycobacterium tuberculosis after application of the Boom DNA extraction method. 2nd Eur Meeting Mol Diagn, Scheveningen, The Netherlands. J Microbiol Methods 47:107
6. Noordhoek GT, van Embden JD, Kolk AH (1996) Reliability of nucleic acid amplification for detection of Mycobacterium tuberculosis: an international collaborative quality control study among 30 laboratories. J Clin Microbiol 34:2522–2525
7. Noordhoek GT, Mulder S, Wallace P, van Loon AM (2004) Multicentre quality control study for detection of Mycobacterium tuberculosis in clinical samples by nucleic amplification methods. Clin Microbiol Infect 10:295–301 DOI: 10.1111/j.1198-743X.2004.00825.x
8. Suffys P, Vanderborght PR, Santos PB, Correa LA, Bravin Y, Kritski AL (2001) Inhibition of the polymerase chain reaction by sputum samples from tuberculosis patients after processing using a silica-guanidiniumthiocyanate DNA isolation procedure. Mem Inst Oswaldo Cruz 96:1137–1139 DOI: 10.1590/S0074-02762001000800019