Rapid detection of mycoplasma in continuous cell lines using a selective biochemical test

Leukemia Research

Volumn 32, Issue 2, 2008, Pages 323-326

  Mariotti, E ^a   Mirabelli, P ^a   Di Noto, R ^a   Fortunato, G ^a   Salvatore, F ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Cell lines; Hematopoietic cell lines; Microbiological agar culture; MycoAlert ; Mycoplasma; PCR ELISA assay

Indexed keywords

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL CULTURE; CHEMICAL ANALYSIS; MYCOPLASMA; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY;

ANIMALS; CELL LINE; DNA, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MICROBIOLOGICAL TECHNIQUES; MYCOPLASMA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 38949092488     PISSN: 01452126     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.leukres.2007.04.010     Document Type: Article

References (9)

1. Van Dongen J.J.M., Macintyre E.A., Gabert J.A., et al. Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukaemia for detection of minimal residual disease. Leukemia 13 (1999) 1901-1928
2. Ogilvie M. Molecular techniques should not now replace cell culture in diagnostic virology laboratories. Rev Med Virol 11 (2001) 351-354
3. Drexler H.G., Uphoff C.C., Dirks W., and MacLeod R.A.F. Mix-ups and mycoplasma: the enemies within. Leukemia Res 26 (2002) 329-333
4. Drexler H.G., and Uphoff C.C. Contamination of cell culture, mycoplasma. In: Spier E., et al. (Ed). Encyclopedia of cell technology (2000), Wiley, New York 609-627
5. Drexler H.G., and Uphoff C.C. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotecnology 39 (2002) 23-38
6. Uphoff C.C., Meyer C., and Drexler H.G. Elimination of mycoplasma from leukaemia-lymphoma cell lines using antibiotics. Leukemia 16 (2002) 284-288
7. Uphoff C.C., and Drexler H.G. Detection of mycoplasma in leukaemia-lymphoma cell lines using polymerase chain reaction. Leukemia 16 (2002) 289-293
8. Uphoff C.C., and Drexler H.G. Detection of mycoplasma contaminations. In: Helgason C.D., and Miller C.L. (Eds). Basic cell culture protocols: methods in molecular biology. 3rd ed. 290 (2005) 13-23
9. Galen R.S., and Gambino S.R. Beyond normality: the predictive value and efficiency of medical diagnosis (1975), John Wiley and Sons, Inc., New York