Purification of α-toxin from Clostridium perfringens type A in PEG-phosphate aqueous two-phase systems: A factorial study

Journal of Chemical Technology and Biotechnology

Volumn 83, Issue 2, 2008, Pages 158-162

  Cavalcanti, Maria T H ^a,b   Porto, Tatiana S ^a,b   Neto, Benicio B ^b   Lima Filho, José L ^b   Porto, Ana L F ^b,c   Pessoa Jr , Adalberto ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

^c UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO   (Brazil)

Author keywords

Alpha toxin; Aqueous two phase systems; Downstream processing; Enzymes; Factorial design; Purification

Indexed keywords

ENZYMES; HYDROPHOBICITY; PHOSPHATES; POLYETHYLENE GLYCOLS; SOLUTIONS;

ALPHA TOXIN; AQUEOUS TWO-PHASE SYSTEMS; CLOSTRIDIUM PERFRINGENS; DOWNSTREAM PROCESSING; FACTORIAL DESIGN;

PURIFICATION;

CLOSTRIDIUM TOXIN; MACROGOL; PHOSPHATE; PHOSPHOLIPASE C; POLYMER;

ENZYMES; HYDROPHOBICITY; PHOSPHATES; POLYETHYLENE GLYCOLS; PURIFICATION; SOLUTIONS;

AQUEOUS SOLUTION; ARTICLE; CHEMICAL INTERACTION; CLOSTRIDIUM PERFRINGENS TYPE A; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; FACTORIAL DESIGN; HYDROPHOBICITY; NONHUMAN; PARTITION COEFFICIENT; PH; PROTEIN PURIFICATION; VOLUMETRY;

CLOSTRIDIUM PERFRINGENS A;

EID: 38849186633     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.1798     Document Type: Article

References (24)

1. Titball RW, Fearn AM and Williamson ED, Biochemical and immunological properties of the C-terminal domain of the alpha-toxin of Clostridium perfringens. FEMS Microbiol Lett 110:45-50 (1993).
2. Titball RW, Membrane-damaging and cytotoxic phospholipases, in The Comprehensive Source Book of Bacterial Protein Toxins, 2nd edn, ed by Joseph EA and Freer JH. Academic Press, New York, pp 310-330 (1999).
3. Ochi S, Oda M, Matsuda H, Ikari S and Sakurai J, Clostridium perfringens α-toxin activates the sphingomyelin metabolism system in sheep erythrocytes. J Biol Chem 279:12181-12189 (2004).
4. Songer JG, Bacterial phospholipases and their role in virulence. Trends Microbiol 5:156-161 (1997).
5. Cavalcanti MTH, Porto TS, Porto ALF, Brandi IV, Lima Filho JL and Pessoa A Jr, Large scale purification of Clostridium perfringens toxin: a review. Brazilian J Pharmacol Sci 40:151-164 (2004).
6. Rito-Palomares M, Practical application of aqueous two-phase partition to process development for the recovery of biological products. J Chromatogr B 807:3-11 (2004).
7. Zhang C, Medina-Bolivar F, Buswell S and Cramer CL, Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction. J Biotechol 117:39-48 (2005).
8. Kurioka S and Matsuda M, Phospholipase C assay using p-nitrophenylphosphorylcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. Anal Biochem 75:281-289 (1976).
9. Bradford MM, A rapid and sensitive method for the quantification of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254 (1976).
10. Hatti-Kaul R (ed), Methods in Biotechnology: Aqueous Two-Phase Systems Methods and Protocols. Humana Press, Totowa NJ, Vol. 11 (2000).
11. Bruns RE, Scarminio IS and Neto BB, Statistical Design-Chemometrics, 1st edn. Elsevier, Amsterdam Vol. 1 (2006).
12. Statsoft Inc. STATISTICA (data analysis software systems) version 6.1 (2004). www.statsoft.com.
13. Cavalcanti MTH, Porto TS, Neto BB, Lima Filho JL, Porto ALF and Pessoa Jr A, Aqueous two-phase systems extraction of α-toxin from Clostridium perfringens type A. J Chromatogr B 833:135-140 (2006).
14. Farrugia B, Nerli B, Di Nucci H, Rigatuso R and Pico G, Thermal features of the bovine serum albumin unfolding by polyethylene glycols. Int J Biol Macromol 26:23-33 (1999).
15. Harris M, Poly (ethylene Glycol) chemistry, Biotechnical Biomedical Applications, ed. by Harris MJ. Plenum Press, New York (1992).
16. Farrugia B, Rigatuso R, Capezio L, Diez V and Pico G, Influence of high concentration monovalent cátions on the protein partitioning in polyethyleneglycol 1500-phosphate aqueous two-phase systems. J Chromatogr B 809:301-306 (2004).
17. Zaslavsky BY, Aqueous two-phase partitioning, in Physical Chemistry and Bioanalytical Applications, ed. by Zaslavsky BY. Marcel Dekker, New York (1995).
18. Duarte MCT, Portugal EP, Ponezi AN, Bim MA, Tagliari CV and Franco TT, Production and purification of alkaline xylanase. Biores Technol 68:49-53 (1999).
19. Falco ALP, Durrant LR and Franco TT, Purification of α-galactosidase from seeds of Sesbania marginata. Brazilian J Chem Eng 17:819-825 (2000).
20. Cortez EV, Felipe MGA, Roberto IC, Pessoa Jr A and Vitolo M, Extraction by reversed micelles of the intracellular enzyme xylose reductase. Appl Biochem Biotechnol 91/93:753-759 (2001).
21. Tubio G, Nerli B and Picó G, Relationship between the protein surface hydrophobicity and its partitioning behavior in aqueous two-phase systems of polyethyleneglycol-dextran. J Chromatogr B 799:293-301 (2004).
22. Farrugia B, Nerli B and Picó G, Study of the serum albumin-polyethyleneglycol interaction to predict the protein partitioning in aqueous two-phase systems. J Chromatogr B 798:25-33 (2003).
23. Grimonprez B and Johansson G, Liquid-liquid partitioning of some enzymes especially phosphofructokinase from Saccharomyces cerevisiae at sub-zero temperature. J Chromatogr B 680:55-63 (1995).
24. Pancera SM, Silva LHM, Loh W, Itri R, Pessoa Jr A and Petri DFS, The effect of poly(ethylene glycol) on the activity and structure of glucose-6-phosphate dehydrogenase in solution. Colloids Surfaces 26:219-300 (2002).