The use of gene transfer technology to study the pathophysiology of erectile dysfunction

Journal of Sexual Medicine

Volumn 5, Issue 2, 2008, Pages 268-275

  Bivalacqua, Trinity J ^a   Strong, Travis D ^a  

^a JOHNS HOPKINS HOSPITAL   (United States)

Author keywords

Endothelium; Gene therapy; Nitric oxide; Nitric oxide synthase; Phosphodiesterase yype 5

Indexed keywords

ADENOVIRUS VECTOR; ENDOTHELIAL NITRIC OXIDE SYNTHASE; LARGE CONDUCTANCE CALCIUM ACTIVATED POTASSIUM CHANNEL ALPHA SUBUNIT; NITRIC OXIDE SYNTHASE; RECOMBINANT PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; RNA; VASCULOTROPIN; VIRUS VECTOR;

ARTICLE; CELL BASED GENE THERAPY; CLINICAL TRIAL; DOWN REGULATION; DRUG EFFICACY; DRUG MECHANISM; ERECTILE DYSFUNCTION; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE SILENCING; GENE TARGETING; GENE THERAPY; GENE TRANSFER; GENETIC IDENTIFICATION; GENETIC TRANSCRIPTION; HUMAN; IMMUNE RESPONSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PATHOGENESIS; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROMOTER REGION; TRANSGENE; VIRAL GENE DELIVERY SYSTEM;

EID: 38649134152     PISSN: 17436095     EISSN: 17436109     Source Type: Journal    
DOI: 10.1111/j.1743-6109.2007.00758.x     Document Type: Article

References (19)

1. Lue TF, Giuliano F, Montorsi R, Rosen RC, Andersson KE, Althof S, Christ G, Hatzichristou D, Hirsch M, Kimoto Y, Lewis R, McKenna K, MacMahon C, Morales A, Mulcahy J, Padma-Nathan H, Pryor J, De Tejada IS, Shabsigh R, Wagner G. Summary of the recommendations on sexual dysfunctions in men. J Sex Med 2004;1:6-23.
2. Kendirci M, Teloken PE, Champion HC, Hellstrom WJG, Bivalacqua TJ. Gene therapy for erectile dysfunction: Fact or fiction? Eur Urol 2006;50:1208-22.
3. Lavigne MD, Górecki DC. Emerging vectors and targeting methods for nonviral gene therapy. Expert Opin Emerg Drugs 2006;11:541-57.
4. Romano G. The controversial role of adenoviral-derived vectors in gene therapy programs: Where do we stand? Drug News Perspect 2006;19:99-106.
5. Sullivan CJ, Teal TH, Luttrell IP, Tran KB, Peters MA, Wessells H. Microarray analysis reveals novel gene expression changes associated with erectile dysfunction in diabetic rats. Physiol Genomics 2005;23:192-205.
6. Christ GJ, Rehman J, Day N, Salkoff L, Valcic M, Melman A, Geliebter J. Intracorporal injection of hSlo cDNA in rats produces physiologically relevant alterations in penile function. Am J Physiol 1998;275:H600-8.
7. Magee TR, Ferrini M, Garban HJ, Vernet D, Mitani K, Rajfer J, Gonzalez-Cadavid NF. Gene therapy of erectile dysfunction in the rat with penile neuronal nitric oxide synthase. Biol Reprod 2002;67:20-8.
8. Bivalacqua TJ, Musicki B, Usta MF, Champion HC, Kadowitz PJ, Burnett AL, Hellstrom WJ. Endothelial nitric oxide synthase gene therapy for erectile dysfunction. Curr Pharm Des 2005;11:4059-67.
9. Bivalacqua TJ, Liu T, Musicki B, Champion HC, Burnett AL. Endothelial nitric oxide synthase keeps erection regulatory function balance in the penis. Eur Urol 2007;51:1732-40.
10. Champion HC, Bivalacqua TJ, Takimoto E, Kass DA, Burnett AL. Phosphodiesterase-5A dysregulation in penile erectile tissue is a mechanism of priapism. Proc Natl Acad Sci USA 2005;102:1661-6.
11. Rogers RS, Graziottin TM, Lin C-S, Kan YW, Lue TF. Intracavernosal vascular endothelial growth factor (VEGF) injection and adeno-associated virus-mediated VEGF gene therapy prevent and reverse venogenic erectile dysfunction in rats. Int J Impot Res 2003;15:26-37.
12. Häggström S, Lissbrant IF, Bergh A, Damber JE. Testosterone induces vascular endothelial growth factor synthesis in the ventral prostate in castrated rats. J Urol 1999;161:1620-5.
13. Christ GJ, Day N, Santizo C, Sato Y, Zhao W, Sclafani T, Bakal R, Salman M, Davies K, Melman A. Intracorporal injection of hSlo cDNA restores erectile capacity in STZ-diabetic F-344 rats in vivo. Am J Physiol Heart Circ Physiol 2004;287:H1544-53.
14. Melman A, Bar-Chama N, McCullough A, Davies K, Christ G. The first human trial for gene transfer therapy for the treatment of erectile dysfunction: Preliminary results. Eur Urol 2005;48:314-8.
15. Bivalacqua TJ, Champion HC, Usta MF, Cellek S, Chitaley K, Webb RC, Lewis RL, Mills TM, Hellstrom WJ, Kadowitz PJ. RhoA/Rho-kinase suppresses endothelial nitric oxide synthase in the penis: A mechanism for diabetes-associated erectile dysfunction. Proc Natl Acad Sci USA 2004;101:9121-6.
16. Bivalacqua TJ, Burnett AL, Hellstrom WJ, Champion HC. Overexpression of arginase in the aged mouse penis impairs erectile function and decreases eNOS activity: Influence of in vivo gene therapy of anti-arginase. Am J Physiol Heart Circ Physiol 2007;292:H1340-51.
17. Magee TR, Kovanecz I, Davila HH, Ferrini MG, Cantini L, Vernet D, Zuniga FI, Rajfer J, Gonzalez-Cadavid NF. Antisense and short hairpin RNA (shRNA) constructs targeting PIN (Protein Inhibitor or NOS) ameliorate aging-related erectile dysfunction in the rat. J Sex Med 2007;4:633-43.
18. Waehler R, Russell SJ, Curiel DT. Engineering targeted viral vectors for gene therapy. Nat Rev Genet 2007;8:573-87.
19. Li LC, Okino ST, Zhao H, Pookot D, Place RF, Urakami S, Enokida H, Dahiya R. Small dsRNAs induce transcriptional activation in human cells. Proc Natl Acad Sci USA 2006;103:17337-42.