Improved quantification of DNA methylation using methylation-sensitive restriction enzymes and real-time PCR

Epigenetics

Volumn 2, Issue 2, 2007, Pages 86-91

  Hashimoto, Ko ^a   Kokubun, Shoichi ^a   Itoi, Eiji ^a   Roach, Helmtrud I ^b  

^a TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b SOUTHAMPTON GENERAL HOSPITAL   (United Kingdom)

Author keywords

DNA methylation; Methylation sensitive restriction enzyme; MMP 13; Quantitative method; Real time PCR

Indexed keywords

GENOMIC DNA; RESTRICTION ENDONUCLEASE; CYTIDYLYL 3' 5' GUANOSINE; CYTIDYLYL-3'-5'-GUANOSINE; DINUCLEOSIDE PHOSPHATE; DNA; UNCLASSIFIED DRUG;

ARTICLE; CELL HETEROGENEITY; CONTROLLED STUDY; CPG ISLAND; DNA METHYLATION; HUMAN; HUMAN CELL; HUMAN TISSUE; PROMOTER REGION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; CHEMISTRY; GENETICS; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RESTRICTION MAPPING;

DINUCLEOSIDE PHOSPHATES; DNA; DNA METHYLATION; DNA RESTRICTION ENZYMES; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RESTRICTION MAPPING;

EID: 38449120061     PISSN: 15592294     EISSN: 15592308     Source Type: Journal    
DOI: 10.4161/epi.2.2.4203     Document Type: Article

References (23)