The impact of process stress on suspended anchorage-dependent mammalian cells as an indicator of likely challenges for regenerative medicines

Biotechnology and Bioengineering

Volumn 99, Issue 2, 2008, Pages 468-474

  Zoro, B J H ^a   Owen, S ^a,b   Drake, R A L ^a,b   Hoare, M ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b The Automation Partnership   (United Kingdom)

Author keywords

Laminar flow; Mammalian cell; Shear stress; Tissue engineering

Indexed keywords

CELL CULTURE; GROWTH RATE; LAMINAR FLOW; METABOLISM; RATS; TISSUE ENGINEERING;

INTACT CELLS; MAMMALIAN CELLS; REGENERATIVE MEDICINES;

CELLS;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL MEMBRANE; CELL METABOLISM; CELL POPULATION; CELL SURVIVAL; CELL TRANSFER; FLOW RATE; GROWTH RATE; NONHUMAN; RAT; REGENERATIVE MEDICINE; SHEAR STRESS; SMOOTH MUSCLE FIBER; STRESS; ANIMAL; AORTA; CYTOLOGY; FLOW KINETICS; IMMOBILIZED CELL; METHODOLOGY; ORGAN CULTURE TECHNIQUE; PHYSIOLOGY;

MAMMALIA; RATTUS;

ANIMALS; AORTA; CELLS, CULTURED; CELLS, IMMOBILIZED; MYOCYTES, SMOOTH MUSCLE; ORGAN CULTURE TECHNIQUES; RATS; RHEOLOGY;

EID: 38449114747     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21544     Document Type: Article

References (23)

1. Abu-Reesh I, Kargi F, 1989. Biological responses of hybridoma cells to defined hydrodynamic shear stress. J Biotechnol 9:167-178.
2. Abu-Reesh I, Kargi F, 1991. Biological responses of hybridoma cells to hydrodynamic shear in an agitated bioreactor. Enzyme Microb Technol 13(11):913-919.
3. Al-Rubeai M, Singh RP, Goldman MH, Emery AN, 1995. Death mechanisms of animal cells in conditions of intensive agitation. Biotechnol Bioeng 45:463-472.
4. Altman SA, Randers L, Rao G, 1993. Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations. Biotechnol Prog 9(6):671-674.
5. Beacham DA, Lian J, Wu G, Konkle BA, Ludlow LB, Shapiro SS, 1999. Arterial shear stress stimulates surface expression of the endothelial glycoprotein 1B complex. J Cell Biochem 73(4):508-521.
6. Born C, Zhang Z, Al-Rubeai M, Thomas CR, 1992. Estimation of disruption of animal cells by laminar shear stress. Biotechnol Bioeng. 40:1004-1010.
7. Carswell KS, Papoutsakis ET, 2000. Culture of human T cells in stirred bioreactors for cellular immunotherapy applications: Shear, proliferation and the IL-2 receptor. Biotechnol Bioeng. 68(3):328-338.
8. Cherry RS, Papoutsakis ET, 1986. Hydrodynamic effects on cells in agitated tissue culture reactors. Bioprocess Eng. 1:29-41.
9. Chisti Y, 2001. Hydrodynamic damage to animal cells. Crit Rev Biotechnol. 21(2):67-110.
10. Jones DA, Smith CW, McIntire LV, 1996. Leucocyte adhesion under flow conditions: Principles important in tissue engineering. Biomaterials 17(3):337-347.
11. Kretzmer G, 2000. Influence of stress on adherent cells. Adv Biochem Eng Biotechnol. 67:123-137.
12. Lawrence MB, Kansas GS, Kunkel EJ, Ley K, 1997. Threshold levels of fluid shear promote leukocyte adhesion through Selectins. J Cell Biol. 136(3):717-727.
13. Lee AA, Graham DA, Dela Cruz S, Ratcliffe A, 2002. Fluid shear stress-induced alignment of cultured vascular smooth muscle cells. J Biomech Eng. 124(1):37-43.
14. Mardikar SH, Niranjan K, 2000. Observations on the shear damage to different animal cells in a concentric cylinder viscometer. Biotechnol Bioeng. 68(6):697-704.
15. Markusen JF, Mason C, Hull DA, Town MA, Tabor AB, Clements M, Boshoff CH, Dunnill P, 2006. Behaviour of adult human mesenchymal stem cells entrapped in alginate-GRGDY beads. Tissue Eng. 12(4):821-830.
16. Mascotti K, McCullough J, Burger SR, 2000. HPC viability measurement: Trypan blue versus acridine orange and propidium iodide. Transfusion 40(6):693-696.
17. McQueen A, Meilhoc E, Bailey JE, 1987. Flow effects on the viability and lysis of suspended mammalian cells. Biotechnol Lett. 9(12):831-836.
18. Olivier LA, Truskey GA, 1993. A numerical analysis of forces exerted by laminar flow on spreading cells in a parallel plate flow chamber assay. Biotechnol Bioeng. 42:963-973.
19. Papadaki M, McIntire LV, Eskin SG, 1996. Effects of shear stress on the growth kinetics of human aortic smooth muscle cells in vitro. Biotechnol Bioeng. 50:555-561.
20. Papoutsakis ET, 1991. Fluid-mechanical damage of animal cells in bioreactors. Trends Biotechnol. 9(12):427-437.
21. Petersen, JF, McIntire LV, Papoutsakis ET, 1988. Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentration. J Biotechnol. 7(3):229-246.
22. Shiragami N, 1997. Effect of shear rate on hybridoma cell metabolism. Bioprocess Eng. 16(6):345-347.
23. Zhang Z, Al-Rubeai M, Thomas CR, 1993. Estimation of disruption of animal cells by turbulent capillary flow. Biotechnol Bioeng. 42:987-993.