Detection of the disease-associated isoform of the prion protein in formalin-fixed tissues by Western blot

Journal of Veterinary Diagnostic Investigation

Volumn 19, Issue 5, 2007, Pages 548-552

  Nicholson, Eric M ^a   Kunkle, Robert A ^a   Hamir, Amir N ^a   Lebepe Mazur, Semakaleng ^a   Orcutt, Dennis ^a  

^a NATIONAL ANIMAL DISEASE CENTER   (United States)

Author keywords

Formalin; Prion; Prion protein (PrP); Scrapie; Spongiform encephalopathy; Transmissible spongiform encephalopathies (TSE); Western blot

Indexed keywords

FORMALDEHYDE; ISOPROTEIN; PRION PROTEIN;

ANIMAL; ANIMAL DISEASE; ARTICLE; BRAIN STEM; CHEMISTRY; SCRAPIE; SHEEP; TIME; TISSUE FIXATION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; BRAIN STEM; FORMALDEHYDE; PROTEIN ISOFORMS; PRPSC PROTEINS; SCRAPIE; SHEEP; TIME FACTORS; TISSUE FIXATION;

EID: 38449114155     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870701900515     Document Type: Article

References (14)

1. mule deer) agent to cattle. J Comp Pathol 134:63-69.
2. Haritani M, Spencer YI, Wells GA: 1994, Hydrated autoclave pretreatment enhancement of prion protein immunoreactivity in formalin-fixed bovine spongiform encephalopathy-affected brain. Acta Neuropathol (Berl) 87:86-90.
3. Ikeda K, Monden T, Kanoh T, et al.: 1998, Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections. J Histochem Cytochem 46:397-403.
4. International Office of Epizootics, Standards Commission: 2000, Chapter 2.3.13 Bovine spongiform encephalopathy. In: Manual of standards for diagnostic tests and vaccines: lists A and B diseases of mammals, birds and bees, 4th ed. pp. 457-466. Office international des epizootics, Paris.
5. Merz PA, Somerville RA, Wisniewski HM, Iqbal K: 1981, Abnormal fibrils from scrapie-infected brain. Acta Neuropathol (Berl) 54:63-74.
6. Miller J, Jenny A, Rhyan J, et al.: 1997, Detection of Mycobacterium bovis in formalin-fixed, paraffin-embedded tissues of cattle and elk by PCR amplification of an IS6110 sequence specific for Mycobacterium tuberculosis complex organisms. J Vet Diagn Invest 9:244-249.
7. Miller JM, Jenny AL, Taylor WD, et al.: 1994, Detection of prion protein in formalin-fixed brain by hydrated autoclaving immunohistochemistry for the diagnosis of scrapie in sheep. J Vet Diagn Invest 6:366-368.
8. Oesch B, Westaway D, Walchli M, et al.: 1985, A cellular gene encodes scrapie PrP 27-30 protein. Cell 40:735-746.
9. Prusiner SB, Scott MR, DeArmond SJ, Cohen FE: 1998, Prion protein biology. Cell 93:337-348.
10. Schaller O, Fatzer R, Stack M, et al.: 1999, Validation of a Western immunoblotting procedure for bovine PrP(Sc) detection and its use as a rapid surveillance method for the diagnosis of bovine spongiform encephalopathy (BSE). Acta Neuropathol (Berl) 98:437-443.
11. Shi SR, Liu C, Balgley BM, et al.: 2006, Protein extraction from formalin-fixed, paraffin-embedded tissue sections: quality evaluation by mass spectrometry. J Histochem Cytochem 54:739-743.
12. Shi SR, Liu C, Taylor CR: 2006, Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval technique: from experiments to hypothesis. J Histochem Cytochem 55: 105-109.
13. Turk E, Teplow DB, Hood LE, Prusiner SB: 1988, Purification and properties of the cellular and scrapie hamster prion proteins. Eur J Biochem 176:21-30.
14. Yamashita S, Okada Y: 2005, Mechanisms of heat-induced antigen retrieval: analyses in vitro employing SDS-PAGE and immunohistochemistry. J Histochem Cytochem 53: 13-21.