Molecular Genetic Structure-Function Analysis of Translation Initiation Factor eIF5B

Methods in Enzymology

Volumn 429, Issue , 2007, Pages 185-201

  Shin, Byung Sik ^a   Dever, Thomas E ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE; INITIATION FACTOR 5; INITIATION FACTOR 5B; UNCLASSIFIED DRUG; CELL EXTRACT; EUKARYOTIC INITIATION FACTOR 5B; EUKARYOTIC INITIATION FACTOR-5B; FUN12 PROTEIN, S CEREVISIAE; INITIATION FACTOR; INITIATION FACTOR 2; SACCHAROMYCES CEREVISIAE PROTEIN;

MOLECULAR GENETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN RNA BINDING; REVIEW; RIBOSOME; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; TRANSLATION INITIATION; ARTICLE; CELL FRACTIONATION; CHEMISTRY; CYTOLOGY; GENE REPRESSION; GENETICS; PHYSIOLOGY; POLYSOME;

SACCHAROMYCES CEREVISIAE;

CELL EXTRACTS; CELL FRACTIONATION; EUKARYOTIC INITIATION FACTOR-2; EUKARYOTIC INITIATION FACTORS; GTP PHOSPHOHYDROLASES; MUTAGENESIS, SITE-DIRECTED; POLYRIBOSOMES; RIBOSOMES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUPPRESSION, GENETIC;

EID: 38449087681     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(07)29009-3     Document Type: Chapter

References (22)

1. Acker M.G., Shin B.S., Dever T.E., and Lorsch J.R. Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining. J. Biol. Chem. 281 (2006) 8469-8475
2. Algire M.A., Maag D., Savio P., Acker M.G., Tarun Jr. S.Z., Sachs A.B., Asano K., Nielsen K.H., Olsen D.S., Phan L., Hinnebusch A.G., and Lorsch J.R. Development and characterization of a reconstituted yeast translation initiation system. RNA 8 (2002) 382-397
3. Algire M.A., Maag D., and Lorsch J.R. Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation. Mol. Cell 20 (2005) 251-262
4. Boeke J.D., Trueheart J., Natsoulis G., and Fink G.R. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154 (1987) 164-175
5. Choi S.K., Lee J.H., Zoll W.L., Merrick W.C., and Dever T.E. Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast. Science 280 (1998) 1757-1760
6. Dey M., Cao C., Dar A.C., Tamura T., Ozato K., Sicheri F., and Dever T.E. Mechanistic link between PKR dimerization, autophosphorylation, and eIF2alpha substrate recognition. Cell 122 (2005) 901-913
7. Fringer J.M., Acker M.G., Fekete C.A., Lorsch J.R., and Dever T.E. Coupled release of eukaryotic translation initiation factors 5B and 1A from 80S ribosomes following subunit joining. Mol. Cell Biol 27 (2007) 2384-2397
8. Hinnebusch A.G. Mechanism and regulation of initiator methionyl-tRNA binding to ribosomes. In: Sonenberg N., Hershey J.W.B., and Mathews M.B. (Eds). "Translational Control of Gene Expression" (2000), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 185-243
9. Iizuka N., Najita L., Franzusoff A., and Sarnow P. Cap-dependent and cap-independent translation by internal initiation of mRNAs in cell extracts prepared from Saccharomyces cerevisiae. Mol. Cell Biol. 14 (1994) 7322-7330
10. Lee J.H., Choi S.K., Roll-Mecak A., Burley S.K., and Dever T.E. Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2. Proc. Natl. Acad. Sci. USA 96 (1999) 4342-4347
11. Lee J.H., Pestova T.V., Shin B.S., Cao C., Choi S.K., and Dever T.E. Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiation. Proc. Natl. Acad. Sci. USA 99 (2002) 16689-16694
12. Maag D., Fekete C.A., Gryczynski Z., and Lorsch J.R. A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon. Mol. Cell 17 (2005) 265-275
13. Mitchell D.A., Marshall T.K., and Deschenes R.J. Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast. Yeast 9 (1993) 715-722
14. Moreno J.M., Kildsgaard J., Siwanowicz I., Mortensen K.K., and Sperling-Petersen H.U. Binding of Escherichia coli initiation factor IF2 to 30S ribosomal subunits: A functional role for the N-terminus of the factor. Biochem. Biophys. Res. Commun. 252 (1998) 465-471
15. Pestova T.V., Lomakin I.B., Lee J.H., Choi S.K., Dever T.E., and Hellen C.U. The joining of ribosomal subunits in eukaryotes requires eIF5B. Nature 403 (2000) 332-335
16. Roll-Mecak A., Cao C., Dever T.E., and Burley S.K. X-Ray structures of the universal translation initiation factor IF2/eIF5B. Conformational changes on GDP and GTP binding. Cell 103 (2000) 781-792
17. Sherman F. Getting started with yeast. Methods Enzymol. 194 (1991) 3-21
18. Shin B.S., Maag D., Roll-Mecak A., Arefin M.S., Burley S.K., Lorsch J.R., and Dever T.E. Uncoupling of initiation factor eIF5B/IF2 GTPase and translational activities by mutations that lower ribosome affinity. Cell 111 (2002) 1015-1025
19. Shin B.S., Acker M.G., Maag D., Kim J.R., Lorsch J.R., and Dever T.E. Intragenic suppressor mutations restore GTPase and translation functions of a eukaryotic initiation factor 5B switch II mutant. Mol. Cell Biol. 27 (2007) 1677-1685
20. Sikorski R.S., and Boeke J.D. In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast. Methods Enzymol. 194 (1991) 302-318
21. Strathern J.N., and Higgins D.R. Recovery of plasmids from yeast into Escherichia coli: Shuttle vectors. Methods Enzymol. 194 (1991) 319-329
22. Tarun Jr. S.Z., and Sachs A.B. A common function for mRNA 5′ and 3′ ends in translation initiation in yeast. Genes Dev. 9 (1995) 2997-3007