Human deafness mutation E385D disrupts the mechanochemical coupling and subcellular targeting of myosin-1a

Biophysical Journal

Volumn 94, Issue 2, 2008, Pages

  Yengo, Christopher M ^a   Ananthanarayanan, Shobana K ^a   Brosey, Chris A ^b   Mao, Suli ^b   Tyska, Matthew J ^b  

^a UNIVERSITY OF NORTH CAROLINA AT CHARLOTTE   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; ENHANCED GREEN FLUORESCENT PROTEIN; MYOSIN; MYOSIN IA; UNCLASSIFIED DRUG; ASPARTIC ACID; GLUTAMIC ACID; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MYO1A PROTEIN, HUMAN; MYOSIN HEAVY CHAIN; MYOSIN I; MYOSIN II;

ACTIN MYOSIN INTERACTION; ANIMAL CELL; ARTICLE; CELL POLARITY; CONTROLLED STUDY; EPITHELIUM CELL; GENE EXPRESSION; GENE MUTATION; MICROVILLUS; MOUSE; NONHUMAN; PERCEPTION DEAFNESS; WILD TYPE; ANIMAL; CELL FRACTIONATION; CHEMISTRY; DICTYOSTELIUM; GENETICS; HEARING IMPAIRMENT; HUMAN; LETTER; MECHANOTRANSDUCTION; METABOLISM; MICROFILAMENT; MUTATION; PROTEIN TRANSPORT;

ACTINS; ANIMALS; ASPARTIC ACID; DEAFNESS; DICTYOSTELIUM; GLUTAMIC ACID; GREEN FLUORESCENT PROTEINS; HUMANS; MECHANOTRANSDUCTION, CELLULAR; MICROFILAMENTS; MUTATION; MYOSIN HEAVY CHAINS; MYOSIN TYPE I; MYOSIN TYPE II; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SUBCELLULAR FRACTIONS;

EID: 38349054763     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.107.122689     Document Type: Article

References (15)

1. Mooseker, M. S., and R. E. Cheney. 1995. Unconventional myosins. Ann. Rev. Cell Dev. Biol. 11:633-675.
2. Skowron, J. F., W. M. Bement, and M. S. Mooseker. 1998. Human brush border myosin-I and myosin-Ic expression in human intestine and Caco-2BBe cells. Cell Motil. Cytoskeleton. 41:308-324.
3. Dumont, R. A., Y. D. Zhao, J. R. Holt, M. Bahler, and P. G. Gillespie. 2002. Myosin-I isozymes in neonatal rodent auditory and vestibular epithelia. J. Assoc. Res. Otolaryngol. 3:375-389.
4. Donaudy, F., A. Ferrara, L. Esposito, R. Hertzano, O. Ben-David, R. E. Bell, S. Melchionda, L. Zelante, K. B. Avraham, and P. Gasparini. 2003. Multiple mutations of MYO1A, a cochlear-expressed gene, in sensorineural hearing loss. Am. J. Hum. Genet. 72:1571-1577.
5. Collins, K., J. R. Sellers, and P. Matsudaira. 1990. Calmodulin dissociation regulates brush border myosin I (110-kD- calmodulin) mechanochemical activity in vitro. J. Cell Biol. 110:1137-1147.
6. Tyska, M. J., and M. S. Mooseker. 2002. MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells. Biophys. J. 82:1869-1883.
7. Cope, M. J. T., J. Whisstock, I. Rayment, and J. Kendrick-Jones. 1996. Conservation within the myosin motor domain: implications for structure and function. Structure. 4:969-987.
8. Smith, C. A., and I. Rayment. 1996. X-ray structure of the magnesium(II).ADP.vanadate complex of the Dictyostelium discoideum myosin motor domain to 1.9 A resolution. Biochemistry. 35:5404-5417.
9. Fisher, A. J., C. A. Smith, J. B. Thoden, R. Smith, K. Sutoh, H. M. Holden, and I. Rayment. 1995. X-ray structures of the myosin motor domain of Dictyostelium discoideum complexed with MgADP.BeFx and MgADP.AlF4-. Biochemistry. 34:8960-8972.
10. Lawson, J. D., E. Pate, I. Rayment, and R. G. Yount. 2004. Molecular dynamics analysis of structural factors influencing back door pi release in myosin. Biophys. J. 86:3794-3803.
11. Murphy, C. T., R. S. Rock, and J. A. Spudich. 2001. A myosin II mutation uncouples ATPase activity from motility and shortens step size. Nat. Cell Biol. 3:311-315.
12. Liu, X., N. Osherov, R. Yamashita, H. Brzeska, E. D. Korn, and G. S. May. 2001. Myosin I mutants with only 1% of wild-type actin-activated MgATPase activity retain essential in vivo function(s). Proc. Natl. Acad. Sci. USA. 98:9122-9127.
13. Tang, N., and E. M. Ostap. 2001. Motor domain-dependent localization of myo1b (myr-1). Curr. Biol. 11:1131-1135.
14. Ruppert, C., J. Godel, R. T.Muller, R. Kroschewski, J. Reinhard, and M. Bahler. 1995. Localization of the rat myosin I molecules myr 1 and myr 2 and in vivo targeting of their tail domains. J. Cell Sci. 108:3775-3786.
15. McConnell, R. E., and M. J. Tyska. 2007. Myosin-1a powers the sliding of apical membrane along microvillar actin bundles. J. Cell Biol. 177:671-681.