Regulation of PDH in human arm and leg muscles at rest and during intense exercise

American Journal of Physiology - Endocrinology and Metabolism

Volumn 294, Issue 1, 2008, Pages

  Kiilerich, Kristian ^a   Birk, Jesper B ^a   Damsgaard, Rasmus ^a,b   Wojtaszewski, Jørgen F P ^a   Pilegaard, Henriette ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b University of Copenhagen   (Denmark)

Author keywords

Muscle type; Pyruvate dehydrogenase; Pyruvate dehydrogenase activity; Pyruvate dehydrogenase phosphorylation

Indexed keywords

GLYCOGEN; LACTIC ACID; PYRUVATE DEHYDROGENASE;

ADULT; ARM MUSCLE; ARTICLE; CLINICAL ARTICLE; CYCLING; DELTOID MUSCLE; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ENZYME REGULATION; EXERCISE; FEMALE; HUMAN; LEG MUSCLE; MALE; MUSCLE BIOPSY; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; TRICEPS BRACHII MUSCLE; VASTUS LATERALIS MUSCLE;

ADULT; ARM; EXERCISE; GLYCOGEN; HUMANS; LACTIC ACID; LEG; MALE; MUSCLE FIBERS, FAST-TWITCH; MUSCLE FIBERS, SLOW-TWITCH; MUSCLE, SKELETAL; PHOSPHORYLATION; PYRUVATE DEHYDROGENASE (LIPOAMIDE); PYRUVATE DEHYDROGENASE COMPLEX; REST;

EID: 38349004608     PISSN: 01931849     EISSN: 15221555     Source Type: Journal    
DOI: 10.1152/ajpendo.00352.2007     Document Type: Article

References (34)

1. Ahlborg G, Jensen-Urstad M. Metabolism in exercising arm vs. leg muscle. Clin Physiol 11: 459-468, 1991.
2. Baldwin KM, Klinkerfuss GH, Terjung RL, Mole PA, Holloszy JO. Respiratory capacity of white, red, and intermediate muscle: adaptative response to exercise. Am J Physiol 222: 373-378, 1972.
3. Bergström J. Muscle electrolytes in man determined by neutron activation analysis on needle biopsy speciments. A study on normal subjects, kidney patients, and paitients with chronic diarrhea. Scand J Clin Lab Invest 68: 1-110, 1962.
4. Brooke MH, Kaiser KK. Muscle fiber types: how many and what kind? Arch Neurol 23: 369-379, 1970.
5. Brooke MH, Kaiser KK. Three "myosin adenosine triphosphatase" systems: the nature of their pH lability and sulfhydryl dependence. J Histochem Cytochem 18: 670-672, 1970.
6. Burgomaster KA, Heigenhauser GJ, Gibala MJ. Effect of short-term sprint interval training on human skeletal muscle carbohydrate metabolism during exercise and time-trial performance. J Appl Physiol 100: 2041-2047, 2006.
7. Bylund AC, Bjuro T, Cederblad G, Holm J, Lundholm K, Sjostroom M, Angquist KA, Schersten T. Physical training in man. Skeletal muscle metabolism in relation to muscle morphology and running ability. Eur J Appl Physiol Occup Physiol 36: 151-169, 1977.
8. Calbet JA, Holmberg HC, Rosdahl H, van Hall G, Jensen-Urstad M, Saltin B. Why do arms extract less oxygen than legs during exercise? Am J Physiol Regul Integr Comp Physiol 289: R1448-R1458, 2005.
9. Cederblad G, Carlin JI, Constantin-Teodosiu D, Harper P, Hultman E. Radioisotopic assays of CoASH and carnitine and their acetylated forms in human skeletal muscle. Anal Biochem 185: 274-278, 1990.
10. Constantin-Teodosiu D, Cederblad G, Hultman E. A sensitive radioisotopic assay of pyruvate dehydrogenase complex in human muscle tissue. Anal Biochem 198: 347-351, 1991.
11. Essen-Gustavsson B, Henriksson J. Enzyme levels in pools of microdissected human muscle fibres of identified type. Adaptive response to exercise. Acta Physiol Scand 120: 505-515, 1984.
12. Gladden LB. Lactate metabolism: a new paradigm for the third millennium. J Physiol 558: 5-30, 2004.
13. Henriksson J, Reitman JS. Time course of changes in human skeletal muscle succinate dehydrogenase and cytochrome oxidase activities and maximal oxygen uptake with physical activity and inactivity. Acta Physiol Scand 99: 91-97, 1977.
14. Kushmerick MJ, Moerland TS, Wiseman RW. Mammalian skeletal muscle fibers distinguished by contents of phosphocreatine, ATP, and Pi. Proc Natl Acad Sci USA 89: 7521-7525, 1992.
15. LeBlanc PJ, Peters SJ, Tunstall RJ, Cameron-Smith D, Heigenhauser GJ. Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle. J Physiol 557: 559-570, 2004.
16. Lowry OH, Passonneau JV. A Flexible System of Enzymatic Analysis. New York: Academic, 1972.
17. Mandarino LJ, Wright KS, Verity LS, Nichols J, Bell JM, Kolterman OG, Beck-Nielsen H. Effects of insulin infusion on human skeletal muscle pyruvate dehydrogenase, phosphofructokinase, and glycogen synthase. Evidence for their role in oxidative and nonoxidative glucose metabolism. J Clin Invest 80: 655-663, 1987.
18. Mourtzakis M, Saltin B, Graham T, Pilegaard H. Carbohydrate metabolism during prolonged exercise and recovery: interactions between pyruvate dehydrogenase, fatty acids, and amino acids. J Appl Physiol 100: 1822-1830, 2006.
19. Olsen DB, Sacchetti M, Dela F, Ploug T, Saltin B. Glucose clearance is higher in arm than leg muscle in type 2 diabetes. J Physiol 565: 555-562, 2005.
20. Patel MS, Korotchkina LG. Regulation of mammalian pyruvate dehydrogenase complex by phosphorylation: complexity of multiple phosphorylation sites and kinases. Exp Mol Med 33: 191-197, 2001.
21. Pettit FH, Pelley JW, Reed LJ. Regulation of pyruvate dehydrogenase kinase and phosphatase by acetyl-CoA/CoA and NADH/NAD ratios. Biochem Biophys Res Commun 65: 575-582, 1975.
22. Pilegaard H, Birk JB, Sacchetti M, Mourtzakis M, Hardie DG, Stewart G, Neufer PD, Saltin B, van Hall G, Wojtaszewski JF. PDH-E1alpha dephosphorylation and activation in human skeletal muscle during exercise: effect of intralipid infusion. Diabetes 55: 3020-3027, 2006.
23. Pilegaard H, Ordway GA, Saltin B, Neufer PD. Transcriptional regulation of gene expression in human skeletal muscle during recovery from exercise. Am J Physiol Endocrinol Metab 279: E806-E814, 2000.
24. Pilegaard H, Saltin B, Neufer PD. Effect of short-term fasting and refeeding on transcriptional regulation of metabolic genes in human skeletal muscle. Diabetes 52: 657-662, 2003.
25. Plomgaard P, Penkowa M, Leick L, Pedersen BK, Saltin B, Pilegaard H. The mRNA expression profile of metabolic genes relative to MHC isoform pattern in human skeletal muscles. J Appl Physiol 101: 817-825, 2006.
26. Putman CT, Spriet LL, Hultman E, Lindinger MI, Lands LC, McKelvie RS, Cederblad G, Jones NL, Heigenhauser GJ. Pyruvate dehydrogenase activity and acetyl group accumulation during exercise after different diets. Am J Physiol Endocrinol Metab 265: E752-E760, 1993.
27. Sacchetti M, Olsen DB, Saltin B, van Hall G. Heterogeneity in limb fatty acid kinetics in type 2 diabetes. Diabetologia 48: 938-945, 2005.
28. Saltin B, Gollnick PD. Skeletal muscle adaptability: significance for metabolism and performance. In: Handbook of Physiology. Skeletal Muscle. Bethesda, MD: Am. Physiol. Soc. 1983, sect. 10, chapt. 19, p. 555-631.
29. Sjodin B, Thorstensson A, Frith K, Karlsson J. Effect of physical training on LDH activity and LDH isozyme pattern in human skeletal muscle. Acta Physiol Scand 97: 150-157, 1976.
30. Stellingwerff T, Watt MJ, Heigenhauser GJ, Spriet LL. Effects of reduced free fatty acid availability on skeletal muscle PDH activation during aerobic exercise. Am J Physiol Endocrinol Metab 284: E589-E596, 2003.
31. Ward GR, Sutton JR, Jones NL, Toews CJ. Activation by exercise of human skeletal muscle pyruvate dehydrogenase in vivo. Clin Sci (Lond) 63: 87-92, 1982.
32. Watt MJ, Heigenhauser GJ, Dyck DJ, Spriet LL. Intramuscular triacylglycerol, glycogen and acetyl group metabolism during 4 h of moderate exercise in man. J Physiol 541: 969-978, 2002.
33. Watt MJ, Heigenhauser GJ, LeBlanc PJ, Inglis JG, Spriet LL, Peters SJ. Rapid upregulation of pyruvate dehydrogenase kinase activity in human skeletal muscle during prolonged exercise. J Appl Physiol 97: 1261-1267, 2004.
34. World Health Organization. Energy and Protein Requirements. Geneva: WHO, 1985.