A tetra-primer polymerase chain reaction approach for the detection of JAK2 V617F mutation

Genetic Testing

Volumn 11, Issue 4, 2007, Pages 463-466

  Koksal, Vedat ^a   Etlik, Ozdal ^a   Arican Baris, S Tugba ^a   Baris, Ibrahim ^b  

^a BURC Molecular Diagnostic Laboratory Turkey   (Turkey)

^b BOGAZICI UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE; JANUS KINASE 2; PHENYLALANINE; PRIMER DNA; VALINE;

ADULT; AGED; ALLELE; AMINO ACID SEQUENCE; ARTICLE; CLINICAL ARTICLE; GENE; GENE SEQUENCE; HUMAN; HUMAN CELL; HUMAN TISSUE; JAK2 GENE; MYELOPROLIFERATIVE DISORDER; POINT MUTATION; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; QUANTITATIVE ANALYSIS; RELIABILITY; SEQUENCE ANALYSIS; TETRAPRIMER POLYMERASE CHAIN REACTION; TITRIMETRY;

AGED; AGED, 80 AND OVER; BIOLOGICAL MARKERS; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; HUMANS; JANUS KINASE 2; MIDDLE AGED; POINT MUTATION; POLYCYTHEMIA VERA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

TETRA;

EID: 38149136662     PISSN: 10906576     EISSN: None     Source Type: Journal    
DOI: 10.1089/gte.2007.0051     Document Type: Article

References (13)

1. Baxter EJ, Scott LM, Campbell PJ, East C, Fourouclas N, Swanton S, Vassiliou GS, Bench AJ, Boyd EM, Curtin N, Scott MA, Erber WN, Green AR; Cancer Genome Project (2005) Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders. Lancet 365:1054-1061.
2. Jones AV, Kreil S, Zoi K, Waghorn K, Curtis C, Zhang L, Score J, Seear R, Chase AJ, Grand FH, White H, Zoi C, Loukopoulos D, Terpos E, Vervessou EC, Schultheis B, Emig M, Ernst T, Lengfelder E, Hehlmann R, Hochhaus A, Oscier D, Silver RT, Reiter A, Cross NC (2005) Widespread occurrence of the JAK2 V617F mutation in chronic myeloproliferative disorders. Blood 106:2162-2168.
3. Kralovics R, Passamonti F, Buser AS, Teo SS, Tiedt R, Passweg JR, Tichelli A, Cazzola M, Skoda RC (2005) A gain-of-function mutation of JAK2 in myeloproliferative disorders. N Engl J Med 352:1779-1790.
4. Kroger N, Badbaran A, Holler E, Hahn J, Kobbe G, Bornhauser M, Reiter A, Zabelina T, Zander AR, Fehse B (2007) Monitoring of the JAK2-V617F mutation by highly sensitive quantitative real-time PCR after allogeneic stem cell transplantation in patients with myelofibrosis. Blood 109:1316-1321.
5. Levine RL, Wadleigh M, Cools J, Ebert BL, Wernig G, Huntly BJ, Boggon TJ, et al. (2005) Activating mutation in the tyrosine kinase JAK2 in polycythemia vera, essential thrombocythemia, and myeloid metaplasia with myelofibrosis. Cancer Cell 7:387-397.
6. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 6:1215.
7. Olsen RJ, Tang Z, Farkas DH, Bernard DW, Zu Y, Chang CC (2006) Detection of the JAK2 (V617F) mutation in myeloproliferative disorders by melting curve analysis using the LightCycler system. Arch Pathol Lab Med 130:997-1003.
8. Poodt J, Fijnheer R, Walsh IB, Hermans MH (2006) A sensitive and reliable semi-quantitative real-time PCR assay to detect JAK2 V617F in blood. Hematol Oncol 24:227-233.
9. Reading NS, Lim MS, Elenitoba-Johnson KS (2006) Detection of acquired Janus kinase 2 V617F mutation in myeloproliferative disorders by fluorescence melting curve analysis. Mol Diagn Ther 10:311-317.
10. Sattler M, Walz C, Crowley BJ, Lengfelder E, Janne PA, Rogers AM, Kuang Y, Distel RJ, Reiter A, Griffin JD (2006) A sensitive high-throughput method to detect activating mutations of Jak2 in peripheral-blood samples. Blood 107:1237-1238.
11. Vannucchi AM, Pancrazzi A, Bogani C, Antonioli E, Guglielmelli P (2006) A quantitative assay for JAK2(V617F) mutation in myeloproliferative disorders by ARMS-PCR and capillary electrophoresis. Leukemia 20:1055-1060.
12. Wolstencroft EC, Hanlon K, Harries LW, Standen GR, Sternberg A, Ellard S (2007) Development of a quantitative real-time polymerase chain reaction assay for the detection of the JAK2 V617F Mutation. J Mol Diagn 9:42-46.
13. Ye S, Dhillon S, Ke X, Collins AR, Day IN (2001) An efficient procedure for genotyping single nucleotide polymorphisms Nucleic Acids Res 29:E88-8.