The effect of TGF-β1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells

Cellular and Molecular Biology Letters

Volumn 12, Issue 3, 2007, Pages 457-472

  Xu, Guo Ping ^a   Li, Qing Quan ^a   Cao, Xi Xi ^a   Chen, Qi ^a   Zhao, Zhong Hua ^a   Diao, Zi Qiang ^a   Xu, Zu De ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

Epithelial mesenchymal transition; Gene transfer; Smad7; Transforming growth factor 1

Indexed keywords

ALPHA ACTIN; BIOLOGICAL MARKER; CYTOKERATIN 19; SMAD7 PROTEIN; TRANSFORMING GROWTH FACTOR BETA1; UVOMORULIN; VIMENTIN;

ANIMAL CELL; ARTICLE; CELL ELONGATION; CELL STRUCTURE; CELL TRANSFORMATION; CONTROLLED STUDY; DOWN REGULATION; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; GENE OVEREXPRESSION; GENE TRANSFER; GENETIC TRANSFECTION; LUNG ALVEOLUS CELL TYPE 2; LUNG ALVEOLUS EPITHELIUM; LUNG FIBROSIS; MESENCHYME; MYOFIBROBLAST; NONHUMAN; PHASE CONTRAST MICROSCOPY; PHENOTYPE; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; ULTRASTRUCTURE; UPREGULATION; WESTERN BLOTTING;

RATTUS;

ANIMALS; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CELL LINE; CELL SHAPE; EPITHELIAL-MESENCHYMAL TRANSITION; GENE TRANSFER TECHNIQUES; PHENOTYPE; PHOSPHORYLATION; PNEUMOCYTES; RATS; RNA, MESSENGER; SMAD2 PROTEIN; SMAD3 PROTEIN; SMAD7 PROTEIN; TRANSFORMING GROWTH FACTOR BETA1; UP-REGULATION;

EID: 38049173227     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.2478/s11658-007-0018-x     Document Type: Article

References (23)

1. Selman, M., King, T.E. and Pardo, A. Idiopathic pulmonary fibrosis: prevailing and evolving hypotheses about its pathogenesis and implications for therapy. Ann. Intern. Med. 134 (2001) 136-151.
2. King, T.E., Schwarz, M.I., Brown, K., Tooze, J.A., Colby, T.V., Waldron, J.A., Flint, A., Thurlbeck, W. and Cherniack, R.M. Idiopathic pulmonary fibrosis: relationship between histopathologic features and mortality. Am. J. Respir. Crit. Care Med. 164 (2001) 1025-1032.
3. Selman, M. and Pardo, A. Idiopathic pulmonary fibrosis: an epithelial/fibroblastic cross-talk disorder. Respir Res. 3 (2002) 3.
4. Yao, H.W., Xie, Q.M., Chen, J.Q., Deng, Y.M. and Tang, H.F. TGF-beta 1 induces alveolar epithelial to mesenchymal cell transition in vitro. Life Sci. 76 (2004) 29-37.
5. Gauldie, J., Kolb, M. and Sime, P.J. A new direction in the pathogenesis of idiopathic pulmonary fibrosis? Respir Res. 3 (2002) 1.
6. Kalluri, R. and Neilson, E.G. Epithelial-mesenchymal transition and its implications for fibrosis. J. Clin. Invest. 112 (2003) 1776-1784.
7. Liu, Y. Epithelial to mesenchymal transition in renal fibrogenesis: athologic significance, molecular mechanism, and therapeutic intervention. J. Am. Soc. Nephrol. 15 (2004) 1-12.
8. Desmouliere, A., Darby, I.A. and Gabbiani, G. Normal and pathologic soft tissue remodeling: role of the myofibroblast, with special emphasis on liver and kidney fibrosis. Lab. Invest. 83 (2003) 1689-1707.
9. Moustakas, A., Pardali, K. and Gaal, A. Mechanisms of TGF-b signaling in regulation of cell growth and differentiation. Immunol. Lett. 82 (2002) 85-91.
10. Desmouliere, A. Factors influencing myofibroblast differentiation during wound healing and fibrosis. Cell Biol. Int. 19 (1995) 471-476.
11. ten Dijke, P., Goumans, M.J., Itoh, F. and Itoh, S. Regulation of cell proliferation by Smad proteins. J. Cell Physiol. 191 (2002) 1-16.
12. Massague, J. and Wotton, D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 19 (2000) 1745-1754.
13. Derynck, R. and Zhang, Y.E. Smad-dependent and Smad-independent pathways in TGF-b family signaling. Nature 425 (2003) 577-584.
14. Lan, H.Y., Mu,W., Tomita, N., Huang, X.R., Li, J.H. and Zhu, H.J. Inhibition of renal fibrosis by gene transfer of inducible Smad7 using Ultrasound-microbubble system in rat UUO model. J. Am. Soc. Nephrol. 14 (2003) 1535-1548.
15. Dooley, S., Hamzavi, J., Breitkopf, K., Wiercinska, E., Said, H.M., Lorenzen, J., ten Dijke, P. and Gressner, A.M. Smad7 prevents activation of hepatic stellate cells and liver fibrosis in rats. Gastroenterology 125 (2003) 178-191.
16. Zavadil, J. and Bottinger, E.P. TGF-beta and epithelial-to-mesenchymal transitions. Oncogene 24 (2005) 5764-5774.
17. Greenburg, G. and Hay, E.D. Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells. J. Cell Biol. 95 (1982) 333-339.
18. Stoker, M. and Perryman, M. An epithelial scatter factor released by embryo fibroblasts. J. Cell Sci. 77 (1985) 209-23.
19. Miettinen, P.J., Ebner, R., Lopez, A.R. and Derynck, R. TGF-beta induced transdifferentiation of mammary epithelial cells to mesenchymal cells: involvement of type I receptors. J. Cell Biol. 127 (1994) 2021-2036.
20. Kalluri, R. and Neilson, E.G. Epithelial-mesenchymal transition and its implications for fibrosis. J. Clin. Invest. 112 (2003) 1776-1784.
21. Saika, S., Kono-Saika, S., Tanaka, T., Yamanaka, O., Ohnishi, Y., Sato, M., Muragaki, Y., Ooshima, A., Yoo, J., Flanders, K.C. and Roberts, A.B. Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice. Lab. Invest. 84 (2004) 1245-1258.
22. Valcourt, U., Kowanetz, M., Niimi, H., Valcourt U., Heldin, C.H. and Moustakas, A. TGF-beta and the Smad signaling pathway support transcriptomic reprogramming during epithelial-mesenchymal cell transition. Mol. Biol. Cell. 16 (2005) 1987-2002.
23. Lan, H.Y., Mu, W., Tomita, N., Huang, X.R., Li, J.H., Zhu, H.J., Morishita, R. and Johnson, R.J. Inhibition of renal fibrosis by gene transfer of inducible Smad7 using ultrasound-microbubble system in rat UUO model. J. Am. Soc. Nephrol. 14 (2003) 1535-1548.