Fluorescent reference strains of bacteria by chromosomal integration of a modified green fluorescent protein gene

Applied Microbiology and Biotechnology

Volumn 77, Issue 6, 2008, Pages 1287-1295

  Pinheiro, L B ^a   Gibbs, M D ^a   Vesey, G ^b   Smith, J J ^c   Bergquist, P L ^a,d  

^a MACQUARIE UNIVERSITY   (Australia)

^b BTF Pty Ltd   (Australia)

^c QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

^d UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CHROMOSOMES; FLUORESCENCE; MUTAGENESIS; PROTEINS;

BACTERIOPHAGE LAMBDA PROMOTERS; FLUORESCENT REFERENCE STRAINS; IN-VITRO-ASSEMBLED TRANSPOSITION COMPLEX; SALMONELLA SPP;

GENETIC ENGINEERING;

GREEN FLUORESCENT PROTEIN;

BACTERIOPHAGE; BACTERIUM; CHROMOSOME; GENE EXPRESSION; GENOME; PROTEIN; ULTRAVIOLET RADIATION;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIAL SURVIVAL; BACTERIOPHAGE LAMBDA; DNA FLANKING REGION; ESCHERICHIA COLI; FLUORESCENCE; GENE EXPRESSION; MUTAGENESIS; NONHUMAN; PROMOTER REGION; SALMONELLA; TRANSPOSON;

AMINO ACIDS; CHROMOSOMES, BACTERIAL; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; FLUORESCENCE; GENE TARGETING; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MUTAGENESIS, INSERTIONAL; RECOMBINANT PROTEINS; SALMONELLA; TEMPERATURE;

BACTERIA (MICROORGANISMS); ENTEROBACTERIA PHAGE LAMBDA; ENTEROBACTERIA PHAGE MU; ESCHERICHIA COLI; SALMONELLA;

EID: 38049171128     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1253-9     Document Type: Article

References (29)

1. Ahn YB, Beudette H, Trevors JT (2001) Survival of a GFP-labeled polychlorinated biphenyl degrading psychrotolerant Pseudomonas spp. in 4 and 22°C soil microcosms. Microbial Ecol 42:614-623
2. Baldridge GD, Burkhardt N, Herron MJ, Kurtti TJ, Munderloh UG (2005) Analysis of fluorescent protein expression in transformants of Rickettsia monacensis, an obligate intracellular tick symbiont. Appl Environ Microbiol 71:2095-2105
3. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263:802-805
4. Cho JC, Kim SJ (1999) Green fluorescent protein-based direct viable count to verify a viable but non-culturable state of Salmonella typhi in environmental samples. J Microbiol Methods 36:227-235
5. Cormack BP, Valdivia RH, Falkow S (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38
6. Crameri A, Whitehorn EA, Tate E, Stemmer WP (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol 14:315-319
7. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387-395
8. Eberl LSR, Ammendola A, Geisenberger O, Earhart R, Molin S, Amann R (1997) Use of green fluorescent protein as marker for ecological studies of activated sludge communities. FEMS Microbiol Lett 149:77-83
9. Errampalli D, Leung K, Cassidy MB, Kostrzynska M, Blears M, Lee H, Trevors JT (1999) Applications of the green fluorescent protein as a molecular marker in environmental microorganisms. J Microbiol Methods 35:187-199
10. Errampalli D, Okamura H, Lee H, Trevors JT, van Elsas JD (1998) Green fluorescent protein as a marker to monitor survival of phenanthrene-mineralizing Pseudomonas sp. UG14Gr in creosote-contaminated soil. FEMS Microbiology Ecol 26:181-191
11. Fratamico PM, Den Y, Strobaugh TP, Palumbo SA (1997) Construction and characterization of E. coli 0157:H7 strains expressing firefly luciferase and green fluorescent protein and their use in survival studies. J. Food Prot 60:1167-1173
12. Goryshin IY, Jendrisak J, Hoffman LM, Meis R, Reznikoff WS (2000) Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes. Nat Biotechnol 18:97-100
13. Hautefort I, Proenca MJ, Hinton JC (2003) Single-copy green fluorescent protein gene fusions allow accurate measurement of Salmonella gene expression in vitro and during infection of mammalian cells. Appl Environ Microbiol 69:7480-7491
14. Heim R, Tsien RY (1996) Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol 6:178-182
15. Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59
16. Koch B, Jensen LE, Nybroe O (2001) A panel of Tn7-based vectors for insertion of the GFP marker gene or for delivery of cloned DNA into Gram-negative bacteria at a neutral chromosomal site. J Microbiol Methods 45:187-195
17. Lamberg A, Nieminen S, Qiao M, Savilahti H (2002) Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage mu. Appl Environ Microbiol 68:705-712
18. Matthysse AG, Stretton S, Dandie C, McClure NC, Goodman AE (1996) Construction of GFP vectors for use in Gram-negative bacteria other than Escherichia coli. FEMS Microbiol Lett 145:87-94
19. Morgan CA, Bigeni P, Herman N, Gauci M, White PA, Vesey G (2004) Production of precise microbiology standards using flow cytometry and freeze drying. Cytometry A 62:162-168
20. Morris DD, Gibbs MD, Chin CW, Koh MH, Wong KK, Allison RW, Nelson PJ, Bergquist PL (1998) Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.1 and action of the gene product on kraft pulp. Appl Environ Microbiol 64:1759-1765
21. Prachaiyo P, McLandsborough LA (2000) A microscopic method to visualize Escherichia coli interaction with beef muscle. J Food Prot 63:427-433
22. Sambrook J, Fritsch EJ, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, New York
23. Schauder B, Blocker H, Frank R, McCarthy JEG (1987) Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region. Gene 52:279-283
24. Scott KP, Mercer DK, Richardson AJ, Melville CM, Glover LA, Flint HJ (2000) Chromosomal integration of the green fluorescent protein gene in lactic acid bacteria and the survival of marked strains in human gut simulations. FEMS Microbiol Lett 182:23-27
25. Scott KP, Mercer DK, Glover LA, Flint HJ (1998) The green fluorescent protein as visible marker for lactic acid bacteria in complex ecosystems. FEMS Microbiol Ecol 26:219-230
26. Siemering KR, Golbik R, Sever R, Haseloff J (1996) Mutations that suppress the thermosensitivity of green fluorescent protein. Curr Biol 6:1653-1663
27. Tombolini R, Unge A, Davy ME, de Bruijn FJ, Jansson J (1997) Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria. FEMS Microbiol Ecol 22:17-28
28. Tresse O, Errampalli D, Kostrzynska M, Leung KT, Lee H, Trevors J, van Elsas JD (1998) Green fluorescent protein as a visual marker in a p-nitrophenol degrading Moraxella sp. FEMS Microbiol Lett 164:187-193
29. Youvan DC, Michel-Beyerle ME (1996) Structure and fluorescence mechanism of GFP. Nat Biotechnol 14:1219-1220