Covalent Assembly of Penicillin Acylase in Mesoporous Silica Based on Macromolecular Crowding Theory* * Supported by the National High Technology Research and Development Program of China (863 Program, No.2006AA02Z211), the National Natural Science Foundation of China (No.20376034), the Natural Science Foundation of Jiangsu Province of China (BK2006181), and...

Chinese Journal of Chemical Engineering

Volumn 15, Issue 6, 2007, Pages 788-790

  WANG, Anming ^a,b   ZHOU, Cheng ^a   WANG, Hua ^a   SHEN, Shubao ^a   XUE, Jianyue ^b   OUYANG, Pingkai ^a  

^a NANJING TECH UNIVERSITY   (China)

^b CHAOHU UNIVERSITY   (China)

Author keywords

cyclodextrin; enzyme immobilization; macromolecule crowding; penicillin acylase

Indexed keywords

ACYLATION; COVALENT BONDS; CYCLODEXTRINS; ENGINEERING RESEARCH; ENZYME IMMOBILIZATION; MACROMOLECULES; SILICA;

MACROMOLECULAR CROWDING THEORY; PENICILLIN ACYLASE;

MESOPOROUS MATERIALS;

ACYLATION; COVALENT BONDS; CYCLODEXTRINS; ENGINEERING RESEARCH; ENZYME IMMOBILIZATION; MACROMOLECULES; MESOPOROUS MATERIALS; SILICA;

EID: 38049139016     PISSN: 10049541     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1004-9541(08)60003-8     Document Type: Article

References (13)

1. Kallenberg A.I., Rantwijk F.V., and Sheldona R.A. "Immobilization of penicillin G acylase: The key to optimum performance". Adv. Synth. Catal. 347 (2005) 905-926
2. Maria Chong A.S., and Zhao X.S. "Design of large-pore mesoporous materials for immobilization of penicillin G acylase biocatalyst". Catal. Today 93-95 (2004) 293-299
3. Zong J., Chen Y.W., and Shen S.B. "Complex biocatalyst assembled by enzyme and inorganic/metal nanoparticles". J. Chem. Ind. Eng. (China) 57 (2006) 1777-1781 (in Chinese)
4. Yin C.H., Liu T., and Tan T.W. "Synthesis of vitamin A esters by immobilized Candida sp. lipase in organic media". Chin. J. Chem. Eng. 14 1 (2006) 81-86
5. Xue P., Lu G.Z., Guo Y., and Wang Y.S. "Synthesis and properties of the hydrophilic copolymer support containing oxirane for immobilized penicillin acylase". Chem. J. Chinese U. 2 (2004) 361-365 (in Chinese)
6. Minton A.P. "The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media". J. Biol. Chem. 276 (2001) 10577-10580
7. Ellis R.J., and Minton A.P. "Join the crowd". Nature 425 (2003) 27-28
8. Norio I., Hiroyoshi M., Kazuki N., Kazuyuki H., and Nobuo T. "Chromatographic characterization of macroporous monolithic silica prepared via sol-gel process". Colloids Surf. A 187/188 (2001) 273-279
9. Tsubokawa N., Ichioka H., Satoh T., Hayashi S., and Fujiki K. "Grafting of 'dendrimer-like' highly branched polymer onto ultrafine silica surface". React. Funct. Polym. 37 (1998) 75-82
10. Bradford M.M.A. "Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding". Anal. Biochem. 72 (1976) 248-250
11. Lei H., Wang W., Chen L.L., Li X.C., Yi B., and Deng L. "The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres". Enzyme Microb. Technol. 35 (2004) 15-21
12. Cheung M.S., and Thirumalai D. "Nanopore-protein interactions dramatically alter stability and yield of the native state in restricted spaces". J. Mol. Biol. 357 (2006) 632-643
13. Cheung M.S., Klimov D., and Thirumalai D. "Molecular crowding enhances native state stability and refolding rates of globular proteins". Proc. Natl. Acad. Sci. USA. 102 (2005) 4753-4758