Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes

Protoplasma

Volumn 232, Issue 1-2, 2007, Pages 79-85

  Forer, A ^a   Spurck, T ^b   Pickett Heaps, J D ^b  

^a YORK UNIVERSITY   (Canada)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Butanedione monoxime; Chromosome movement; Crane fly spermatocyte; Cytochalasin; Latrunculin; Spindle function; Tubulin flux

Indexed keywords

2,3 BUTANEDIONE; ACTIN; CYTOCHALASIN D; DIACETYL OXIME; DRUG DERIVATIVE; FUSED HETEROCYCLIC RINGS; LATRUNCULIN A; MYOSIN; THIAZOLIDINE DERIVATIVE;

ANIMAL; ARTICLE; CENTROMERE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; FLY; MALE; METABOLISM; METAPHASE; RADIATION EXPOSURE; SPERMATOCYTE; ULTRAVIOLET RADIATION;

ACTINS; ANIMALS; BICYCLO COMPOUNDS, HETEROCYCLIC; CYTOCHALASIN D; DIACETYL; DIPTERA; KINETOCHORES; MALE; METAPHASE; MYOSINS; SPERMATOCYTES; THIAZOLIDINES; ULTRAVIOLET RAYS;

TIPULIDAE;

EID: 38049071151     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-007-0265-8     Document Type: Article

References (33)

1. LA Cameron G Yang D Cimini JC Canman O Kisurina-Evgenieva A Khodjakov G Danuser ED Salmon 2006 Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells J Cell Biol 173 173 179
2. W Chen D Zhang 2004 Kinetochore fibre dynamics outside the context of the spindle during anaphase Nat Cell Biol 6 227 231
3. BB Czaban A Forer AS Bajer 1993 Ultraviolet microbeam irradiation of chromosomal spindle fibres in Haemanthus katherinae endosperm. I. Behaviour of the irradiated region J Cell Sci 105 571 578
4. L Fabian A Forer 2005 Redundant mechanisms for anaphase chromosome movements: crane-fly spermatocyte spindles normally use actin filaments but also can function without them Protoplasma 225 169 184
5. A Forer 1965 Local reduction of spindle fiber birefringence in living Nephrotoma suturalis (Loew) spermatocytes induced by ultraviolet microbeam irradiation J Cell Biol 25 95 117
6. A Forer 1966 Characterization of the mitotic traction system, and evidence that birefringent spindle fibers neither produce nor transmit force for chromosome movement Chromosoma 19 44 98
7. A Forer L Fabian 2005 Does 2,3-butanedione monoxime inhibit nonmuscle myosin? Protoplasma 225 1 4
8. A Forer JD Pickett-Heaps 1998 Cytochalasin D and latrunculin affect chromosome behaviour during meiosis in crane-fly spermatocytes Chromosome Res 6 533 549
9. A Forer JD Pickett-Heaps 2005 Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation Cell Biol Int 29 721 730
10. A Forer PJ Wilson 1994 A model for chromosome movement during mitosis Protoplasma 179 95 105
11. A Forer T Spurck JD Pickett-Heaps 1997 Ultraviolet microbeam irradiations of spindle fibres in crane-fly spermatocytes and newt epithelial cells: resolution of previously conflicting observations Protoplasma 197 230 240
12. A Forer T Spurck JD Pickett-Heaps PJ Wilson 2003 Structure of kinetochore fibres in crane-fly spermatocytes after irradiation with an ultraviolet microbeam: neither microtubules nor actin filaments remain in the irradiated region Cell Motil Cytoskeleton 56 173 192
13. NJ Ganem K Upton DA Compton 2005 Efficient mitosis in human cells lacking poleward microtubule flux Curr Biol 15 1827 1832
14. JR LaFountain Jr CS Cohan AJ Siegel DJ LaFountain 2004 Direct visualization of microtubule flux during metaphase and anaphase in crane-fly spermatocytes Mol Biol Cell 15 5724 5732
15. H Maiato CL Rieder A Khodjakov 2004 Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis J Cell Biol 167 831 840
16. H Maiato A Khodjakov CL Rieder 2005 Drosophila CLASP is required for the incorporation of microtubule subunits into fluxing kinetochore fibres Nat Cell Biol 7 42 47
17. RL Margolis L Wilson 1978 Opposite end assembly and disassembly of microtubules at steady state in vitro Cell 13 1 8
18. RL Margolis L Wilson 1981 Microtubule treadmills - possible molecular machinery Nature 203 705 711
19. TJ Mitchison 1989 Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence J Cell Biol 109 637 652
20. DT Miyamoto ZE Perlman KS Burbank AC Groen TJ Mitchison 2004 The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles J Cell Biol 167 813 818
21. JD Pickett-Heaps A Forer T Spurck 1996 Rethinking anaphase: where "Pac-Man" fails and why a role for the spindle matrix is likely Protoplasma 192 1 10
22. JD Pickett-Heaps A Forer T Spurck 1997 Traction fibre: toward a "tensegral" model of the spindle Cell Motil Cytoskeleton 37 1 6
23. GC Rogers SL Rogers TA Schwimmer SC Ems-McClung CE Walczak RD Vale JM Scholey DJ Sharp 2004 Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase Nature 427 364 370
24. GC Rogers SL Rogers DJ Sharp 2005 Spindle microtubules in flux J Cell Sci 118 1105 1116
25. JM Scholey GC Rogers DJ Sharp 2001 Mitosis, microtubules, and the matrix J Cell Biol 154 261 266
26. PJ Sillers A Forer 1983 Action spectrum for changes in spindle fibre birefringence after ultraviolet microbeam irradiations of single chromosomal spindle fibres in crane-fly spermatocytes J Cell Sci 62 1 25
27. RV Silverman-Gavrila A Forer 2000 Evidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes J Cell Sci 113 597 609
28. T Spurck A Forer JD Pickett-Heaps 1997 Ultraviolet microbeam irradiations of epithelial and spermatocyte spindles suggest that forces act on the kinetochore fibre and are not generated by its disassembly Cell Motil Cytoskeleton 36 136 148
29. PJ Wilson A Forer 1989a The behaviour of microtubules in chromosomal spindle fibres irradiated singly or doubly with ultraviolet light J Cell Sci 94 625 634
30. PJ Wilson A Forer 1989b Identifying the site of microtubule polymerization during regrowth of UV-sheared kinetochore fibres using antibodies against acetylated alpha-tubulin Cell Biol Int Rep 13 823 832
31. PJ Wilson A Forer 1997 Effects of nanomolar taxol on crane-fly spermatocyte spindles indicate that acetylation of kinetochore microtubules can be used as a marker of poleward tubulin flux Cell Motil Cytoskeleton 37 20 32
32. PJ Wilson A Forer C Leggiadro 1994 Evidence that kinetochore microtubules in crane-fly spermatocytes disassemble during anaphase primarily at the poleward end J Cell Sci 107 3015 3027
33. D Zhang RB Nicklas 1996 'Anaphase' and cytokinesis in the absence of chromosomes Nature 382 466 468