Use of recombinant antigens to detect antibodies against Mycoplasma suis, with correlation of serological results to hematological findings

Clinical and Vaccine Immunology

Volumn 14, Issue 12, 2007, Pages 1616-1622

  Hoelzle, Katharina ^a   Grimm, Julia ^b   Ritzmann, Mathias ^b   Heinritzi, Karl ^b   Torgerson, Paul ^a   Hamburger, Anja ^a   Wittenbrink, Max M ^a   Hoelzle, Ludwig E ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BACTERIUM ANTIBODY; HEMOGLOBIN; RECOMBINANT ANTIGEN; RECOMBINANT PROTEIN;

ANAPLASMOSIS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY DETECTION; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTE COUNT; ESCHERICHIA COLI; HEMAGGLUTINATION; MYCOBACTERIOSIS; MYCOPLASMA SUIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEROLOGY; SWINE;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIBODY FORMATION; ANTIGENS, BACTERIAL; BIOLOGICAL MARKERS; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTE INDICES; ESCHERICHIA COLI; IMMUNOBLOTTING; KINETICS; MYCOPLASMA; MYCOPLASMA INFECTIONS; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; SPECIES SPECIFICITY; SPLENECTOMY; STATISTICS AS TOPIC; SWINE; SWINE DISEASES;

EID: 38049017989     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00345-07     Document Type: Article

References (19)

1. Baljer, G., K. Heinritzi, and L. Wieler. 1989. Indirect hemagglutination for Eperythrozoon suis detection in experimentally and spontaneously infected swine. J. Vet. Med. B Infect. Dis. Vet. Public Health. 36:417-423. (In German.)
2. Brownback, A. 1981. Eperythrozoonosis as a cause of infertility in swine. Vet. Med. 76:375-378.
3. Gardner, I. A., H. Stryhn, P. Lind, and M. T. Collins. 2000. Conditional dependence between tests affects the diagnosis and surveillance of animal diseases. Prev. Vet. Med. 45:107-122.
4. Hoelzle, L. E., D. Adelt, K. Hoelzle, K. Heinritzi, and M. M. Wittenbrink. 2003. Development of a diagnostic PCR assay based on novel DNA sequences for the detection of Mycoplasma suis (Eperythrozoon suis) in porcine blood. Vet. Microbiol. 93:185-196.
5. Hoelzle, L. E., K. Hoelzle, M. Ritzmann, K. Heinritzi, and M. M. Wittenbrink. 2006. Mycoplasma suis antigens recognized during humoral immune response in experimentally infected pigs. Clin. Vaccine Immunol. 13:116-122.
6. Hoelzle, L. E., K. Hoelzle, A. Harder, M. Ritzmann, H. Aupperle, H. A. Schoon, K. Heinritzi, and M. M. Wittenbrink. 2007. First identification and functional characterization of an immunogenic protein in unculturable haemotrophic mycoplasmas (Mycoplasma suis HspA1). FEMS Immunol. Med. Microbiol. 49:215-223.
7. Hoelzle, L. E., K. Hoelzle, M. Helbling, H. Aupperle, H. A. Schoon, M. Ritzmann, K. Heinritzi, K. M. Felder, and M. M. Wittenbrink. 2007. MSG1, a surface-localised protein of Mycoplasma suis is involved in the adhesion to erythrocytes. Microbes Infect. 9:466-474.
8. Hoelzle, L. E., M. Helbling, K. Hoelzle, M. Ritzmann, K. Heinritzi, and M. M. Wittenbrink. 2007. First LightCycler real-time PCR assay for the quantitative detection of Mycoplasma suis in clinical samples. J. Microbiol. Methods 70:346-354.
9. Hsu, F. S., M. C. Liu, S. M. Chou, J. F. Zachary, and A. R. Smith. 1992. Evaluation of an enzyme-linked immunosorbent assay for detection of Eperythrozoon suis antibodies in swine. Am. J. Vet. Res. 53:352-354.
10. Kixmoeller, M., M. Ritzmann, and K. Heinritzi. 2006. Reference ranges for laboratory parameters in pigs of different breeds. Prakt. Tierärztl. 87:204-213.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
12. Landis, J. R., and G. G. Koch. 1977. The measurement of observer agreement for categorial data. Biometrics 33:159-174.
13. Messick, J. B. 2004. Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet. Clin. Pathol. 33:2-13.
14. Schuller, W., K. Heinritzi, S. Al-Nuktha, S. Kölbl, and M. Schuh. 1990. Serologic progression studies using CF and ELISA for the detection of antibodies against Eperythrozoon suis infection of swine. Berl. Münch. Tierärztl. Wochenschr. 103:9-12. (In German.)
15. Smith, A. R., and T. Rahn. 1975. An indirect hemagglutination test for the diagnosis of Eperythrozoon suis infection in swine. Am. J. Vet. Res. 36:1319-1321.
16. Splitter, E. J. 1958. The complement-fixation test in diagnosis of eperythrozoonosis in swine. J. Am. Vet. Med. Assoc. 132:47-49.
17. Venzon, D. J., and S. H. Moolgavkar. 1988. A method for computing profile-likelihood-based confidence intervals. Appl. Stat. 37:87-94.
18. Zachary, J. F., and A. R. Smith. 1985. Experimental porcine eperythrozoonosis: T-lymphocyte suppression and misdirected immune responses. Am. J. Vet. Res. 46:821-830.
19. Zinn, G. M., G. W. Jesse, and A. W. Dobson. 1983. Effect of eperythrozoonosis on sow productivity. J. Am. Vet. Med. Assoc. 182:369-371.