Identification and evaluation of a highly effective fusion inhibitor for human metapneumovirus

Antimicrobial Agents and Chemotherapy

Volumn 52, Issue 1, 2008, Pages 279-287

  Deffrasnes, Céline ^a   Hamelin, Marie Ève ^a   Prince, Gregory A ^b   Boivin, Guy ^a  

^a LAVAL UNIVERSITY   (Canada)

^b Virion Systems Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE; CYTOKINE; GAMMA INTERFERON; HYBRID PROTEIN; MONOCYTE CHEMOTACTIC PROTEIN 1; POLYPEPTIDE; RANTES; VIRUS PROTEIN;

AIRWAY OBSTRUCTION; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BAGG ALBINO MOUSE; CONTROLLED STUDY; DRUG CYTOTOXICITY; ESCHERICHIA COLI; FEMALE; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN METAPNEUMOVIRUS; IN VITRO STUDY; IN VIVO STUDY; MORTALITY; MOUSE; NONHUMAN; PARAINFLUENZA VIRUS 3; PARAMYXOVIRUS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; VIRUS INHIBITION; VIRUS LOAD; VIRUS PNEUMONIA;

AMINO ACID SEQUENCE; ANIMALS; ANTIVIRAL AGENTS; CELL LINE; CELL LINE, TUMOR; FEMALE; HUMANS; INHIBITORY CONCENTRATION 50; MEMBRANE FUSION; METAPNEUMOVIRUS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PARAMYXOVIRIDAE INFECTIONS; PEPTIDES; VIRAL FUSION PROTEINS;

EID: 37849034056     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.00793-07     Document Type: Article

References (49)

1. Alvarez, R., K. S. Harrod, W. J. Shieh, S. Zaki, and R. A. Tripp. 2004. Human metapneumovirus persists in BALB/c mice despite the presence of neutralizing antibodies. J. Virol. 78:14003-14011.
2. Boivin, G., Y. Abed, G. Pelletier, L. Ruel, D. Moisan, S. Cote, T. C. Peret, D. D. Erdman, and L. J. Anderson. 2002. Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups. J. Infect. Dis. 186:1330-1334.
3. Boivin, G., G. De Serres, M. E. Hamelin, S. Cote, M. Argouin, G. Tremblay, R. Maranda-Aubut, C. Sauvageau, M. Ouakki, N. Boulianne, and C. Couture. 2007. An outbreak of severe respiratory tract infection due to human metapneumovirus in a long-term care facility. Clin. Infect. Dis. 44:1152-1158.
4. Boivin, G., I. Mackay, T. P. Sloots, S. Madhi, F. Freymuth, D. Wolf, Y. Shemer-Avni, H. Ludewick, G. C. Gray, and E. LeBlanc. 2004. Global genetic diversity of human metapneumovirus fusion gene. Emerg. Infect. Dis. 10:1154-1157.
5. Branden, C., and J. Tooze. 1999. Introduction to protein structure, 2nd ed., p. 14-46. Garland Publishing, New York, NY.
6. Buchholz, U. J., K. Nagashima, B. R. Murphy, and P. L. Collins. 2006. Live vaccines for human metapneumovirus designed by reverse genetics. Expert Rev. Vaccines 5:695-706.
7. Cane, P. A., B. G. van den Hoogen, S. Chakrabarti, C. D. Fegan, and A. D. Osterhaus. 2003. Human metapneumovirus in a haematopoietic stem cell transplant recipient with fatal lower respiratory tract disease. Bone Marrow Transplant. 31:309-310.
8. Cooper, D. A., and J. M. Lange. 2004. Peptide inhibitors of virus-cell fusion: enfuvirtide as a case study in clinical discovery and development. Lancet Infect. Dis. 4:426-436.
9. Cseke, G., D. W. Wright, S. J. Tollefson, J. E. Johnson, J. E. Crowe, Jr., and J. V. Williams. 2007. Human metapneumovirus fusion protein vaccines that are immunogenic and protective in cotton rats. J. Virol. 81:698-707.
10. Darniot, M., T. Petrella, S. Aho, P. Pothier, and C. Manoha. 2005. Immune response and alteration of pulmonary function after primary human metapneumovirus (hMPV) infection of BALB/c mice. Vaccine 23:4473-4480.
11. Deffrasnes, C., M. E. Hamelin, and G. Boivin. 2007. Human metapneumovirus. Semin. Respir. Crit. Care Med. 28:213-221.
12. Easton, A. J., J. B. Domachowske, and H. F. Rosenberg. 2004. Animal pneumoviruses: molecular genetics and pathogenesis. Clin. Microbiol. Rev. 17:390-412.
13. Englund, J. A., M. Boeckh, J. Kuypers, W. G. Nichols, R. C. Hackman, R. A. Morrow, D. N. Fredricks, and L. Corey. 2006. Brief communication: fatal human metapneumovirus infection in stem-cell transplant recipients. Ann. Intern. Med. 144:344-349.
14. Hamelin, M. E., G. A. Prince, and G. Boivin. 2006. Effect of ribavirin and glucocorticoid treatment in a mouse model of human metapneumovirus infection. Antimicrob. Agents Chemother. 50:774-777.
15. Hamelin, M. E., G. A. Prince, A. M. Gomez, R. Kinkead, and G. Boivin. 2006. Human metapneumovirus infection induces long-term pulmonary inflammation associated with airway obstruction and hyperresponsiveness in mice. J. Infect. Dis. 193:1634-1642.
16. Hamelin, M. E., K. Yim, K. H. Kuhn, R. P. Cragin, M. Boukhvalova, J. C. Blanco, G. A. Prince, and G. Boivin. 2005. Pathogenesis of human metapneumovirus lung infection in BALB/c mice and cotton rats. J. Virol. 79:8894-8903.
17. Joshi, S. B., R. E. Dutch, and R. A. Lamb. 1998. A core trimer of the paramyxovirus fusion protein: parallels to influenza virus hemagglutinin and HIV-1 gp41. Virology 248:20-34.
18. Kilby, J. M., and J. J. Eron. 2003. Novel therapies based on mechanisms of HIV-1 cell entry. N. Engl. J. Med. 348:2228-2238.
19. Kilby, J. M., S. Hopkins, T. M. Venetta, B. DiMassimo, G. A. Cloud, J. Y. Lee, L. Alldredge, E. Hunter, D. Lambert, D. Bolognesi, T. Matthews, M. R. Johnson, M. A. Nowak, G. M. Shaw, and M. S. Saag. 1998. Potent suppression of HIV-1 replication in humans by T-20, a peptide inhibitor of gp41-mediated virus entry. Nat. Med. 4:1302-1307.
20. Krambovitis, E., F. Porichis, and D. A. Spandidos. 2005. HIV entry inhibitors: a new generation of antiretroviral drugs. Acta Pharmacol. Sin. 26:1165-1173.
21. Lamb, R. A., R. G. Paterson, and T. S. Jardetzky. 2006. Paramyxovirus membrane fusion: lessons from the F and HN atomic structures. Virology 344:30-37.
22. Lambert, D. M., S. Barney, A. L. Lambert, K. Guthrie, R. Medinas, D. E. Davis, T. Bucy, J. Erickson, G. Merutka, and S. R. Petteway, Jr. 1996. Peptides from conserved regions of paramyxovirus fusion (F) proteins are potent inhibitors of viral fusion. Proc. Natl. Acad. Sci. USA 93:2186-2191.
23. Lawless-Delmedico, M. K., P. Sista, R. Sen, N. C. Moore, J. B. Antczak, J. M. White, R. J. Greene, K. C. Leanza, T. J. Matthews, and D. M. Lambert. 2000. Heptad-repeat regions of respiratory syncytial virus F1 protein form a six-membered coiled-coil complex. Biochemistry 39:11684-11695.
24. Lomask, M. 2006. Further exploration of the Penh parameter. Exp. Toxicol. Pathol. 57(Suppl. 2):13-20.
25. Lu, M., S. C. Blacklow, and P. S. Kim. 1995. A trimeric structural domain of the HIV-1 transmembrane glycoprotein. Nat. Struct. Biol. 2:1075-1082.
26. Mackay, I. M., S. Bialasiewicz, Z. Waliuzzaman, G. R. Chidlow, D. C. Fegredo, S. Laingam, P. Adamson, G. B. Harnett, W. Rawlinson, M. D. Nissen, and T. P. Sloots. 2004. Use of the P gene to genotype human metapneumovirus identifies 4 viral subtypes. J. Infect. Dis. 190:1913-1918.
27. Maertzdorf, J., C. K. Wang, J. B. Brown, J. D. Quinto, M. Chu, M. de Graaf, B. G. van den Hoogen, R. Spaete, A. D. Osterhaus, and R. A. Fouchier. 2004. Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages. J. Clin. Microbiol. 42:981-986.
28. Miller, S. A., S. Tollefson, J. E. Crowe, Jr., J. V. Williams, and D. W. Wright. 2007. Examination of a fusogenic hexameric core from human metapneumovirus and identification of a potent synthetic peptide inhibitor from the heptad repeat 1 region. J. Virol. 81:141-149.
29. Morrison, T. G. 2003. Structure and function of a paramyxovirus fusion protein. Biochim. Biophys. Acta 1614:73-84.
30. Morrow, B. M., M. Hatherill, H. E. Smuts, J. Yeats, R. Pitcher, and A. C. Argent. 2006. Clinical course of hospitalised children infected with human metapneumovirus and respiratory syncytial virus. J. Paediatr. Child Health 42:174-178.
31. Njenga, M. K., H. M. Lwamba, and B. S. Seal. 2003. Metapneumoviruses in birds and humans. Virus Res. 91:163-169.
32. Peret, T. C., G. Boivin, Y. Li, M. Couillard, C. Humphrey, A. D. Osterhaus, D. D. Erdman, and L. J. Anderson. 2002. Characterization of human metapneumoviruses isolated from patients in North America. J. Infect. Dis. 185:1660-1663.
33. Porotto, M., L. Doctor, P. Carta, M. Fornabaio, O. Greengard, G. E. Kellogg, and A. Moscona. 2006. Inhibition of Hendra virus fusion. J. Virol. 80:9837-9849.
34. Prince, G. A., J. P. Prieels, M. Slaoui, and D. D. Porter. 1999. Pulmonary lesions in primary respiratory syncytial virus infection, reinfection, and vaccine-enhanced disease in the cotton rat (Sigmodon hispidus). Lab. Investig. 79:1385-1392.
35. Pyrc, K., B. J. Bosch, B. Berkhout, M. F. Jebbink, R. Dijkman, P. Rottier, and L. van der Hoek. 2006. Inhibition of human coronavirus NL63 infection at early stages of the replication cycle. Antimicrob. Agents Chemother. 50:2000-2008.
36. Russell, C. J., T. S. Jardetzky, and R. A. Lamb. 2001. Membrane fusion machines of paramyxoviruses: capture of intermediates of fusion. EMBO J. 20:4024-4034.
37. Skiadopoulos, M. H., S. Biacchesi, U. J. Buchholz, J. M. Riggs, S. R. Surman, E. Amaro-Carambot, J. M. McAuliffe, W. R. Elkins, M. St. Claire, P. L. Collins, and B. R. Murphy. 2004. The two major human metapneumovirus genetic lineages are highly related antigenically, and the fusion (F) protein is a major contributor to this antigenic relatedness. J. Virol. 78:6927-6937.
38. Smith, B. J., M. C. Lawrence, and P. M. Colman. 2002. Modelling the structure of the fusion protein from human respiratory syncytial virus. Protein Eng. 15:365-371.
39. Ulbrandt, N. D., H. Ji, N. K. Patel, J. M. Riggs, Y. A. Brewah, S. Ready, N. E. Donacki, K. Folliot, A. S. Barnes, K. Senthil, S. Wilson, M. Chen, L. Clarke, M. MacPhail, J. Li, R. M. Woods, K. Coelingh, J. L. Reed, M. P. McCarthy, D. S. Pfarr, A. D. Osterhaus, R. A. Fouchier, P. A. Kiener, and J. A. Suzich. 2006. Isolation and characterization of monoclonal antibodies which neutralize human metapneumovirus in vitro and in vivo. J. Virol. 80:7799-7806.
40. van den Hoogen, B. G., T. M. Bestebroer, A. D. Osterhaus, and R. A. Fouchier. 2002. Analysis of the genomic sequence of a human metapneumovirus. Virology 295:119-132.
41. van den Hoogen, B. G., J. C. de Jong, J. Groen, T. Kuiken, R. de Groot, R. A. Fouchier, and A. D. Osterhaus. 2001. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat. Med. 7:719-724.
42. van den Hoogen, B. G., S. Herfst, L. Sprong, P. A. Cane, E. Forleo-Neto, R. L. de Swart, A. D. Osterhaus, and R. A. Fouchier. 2004. Antigenic and genetic variability of human metapneumoviruses. Emerg. Infect. Dis. 10:658-666.
43. Vargas, S. O., H. P. Kozakewich, A. R. Perez-Atayde, and A. J. McAdam. 2004. Pathology of human metapneumovirus infection: insights into the pathogenesis of a newly identified respiratory virus. Pediatr. Dev. Pathol. 7:478-486.
44. Wang, E., X. Sun, Y. Qian, L. Zhao, P. Tien, and G. F. Gao. 2003. Both heptad repeats of human respiratory syncytial virus fusion protein are potent inhibitors of viral fusion. Biochem. Biophys. Res. Commun. 302:469-475.
45. Wang, X. J., Y. D. Bai, G. Z. Zhang, J. X. Zhao, M. Wang, and G. F. Gao. 2005. Structure and function study of paramyxovirus fusion protein heptad repeat peptides. Arch. Biochem. Biophys. 436:316-322.
46. Wild, C. T., D. C. Shugars, T. K. Greenwell, C. B. McDanal, and T. J. Matthews. 1994. Peptides corresponding to a predictive alpha-helical domain of human immunodeficiency virus type 1 gp41 are potent inhibitors of virus infection. Proc. Natl. Acad. Sci. USA 91:9770-9774.
47. Williams, J. V., Z. Chen, G. Cseke, D. W. Wright, C. J. Keefer, S. J. Tollefson, A. Hessell, A. Podsiad, B. E. Shepherd, P. P. Sanna, D. R. Burton, J. E. Crowe, Jr., and R. A. Williamson. 2007. A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo. J. Virol. 81:8315-8324.
48. Yao, Q., and R. W. Compans. 1996. Peptides corresponding to the heptad repeat sequence of human parainfluenza virus fusion protein are potent inhibitors of virus infection. Virology 223:103-112.
49. Yin, H. S., R. G. Paterson, X. Wen, R. A. Lamb, and T. S. Jardetzky. 2005. Structure of the uncleaved ectodomain of the paramyxovirus (hPIV3) fusion protein. Proc. Natl. Acad. Sci. USA 102:9288-9293.