Comparison and validation of methods to quantify Cry1Ab toxin from Bacillus thuringiensis for standardization of insect bioassays

Applied and Environmental Microbiology

Volumn 74, Issue 1, 2008, Pages 130-135

  Crespo, André L B ^a   Spencer, Terence A ^a   Nekl, Emily ^b   Pusztai Carey, Marianne ^c   Moar, William J ^d   Siegfried, Blair D ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b HIGH POINT UNIVERSITY   (United States)

^c CASE WESTERN RESERVE UNIVERSITY   (United States)

^d AUBURN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DENSITOMETERS; ELECTROPHORESIS; ENZYME ACTIVITY; GENES; INSECTICIDES; PROTEINS;

BACILLUS THURINGIENSIS; INSECT BIOASSAYS;

BIOASSAY;

CRY1AB TOXIN;

BACTERIUM; BIOASSAY; COMPARATIVE STUDY; CONCENTRATION (COMPOSITION); INSECT; LABORATORY METHOD; MAIZE; PROTEIN; STANDARDIZATION; TOXIN; TRANSGENIC PLANT;

ARTICLE; BACILLUS THURINGIENSIS; BIOASSAY; DENSITOMETRY; ENZYME LINKED IMMUNOSORBENT ASSAY; EUROPEAN CORN BORER; IMMUNOBLOTTING; INSECT; INTERMETHOD COMPARISON; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; TOXIN ANALYSIS; VALIDATION STUDY;

ANALYSIS OF VARIANCE; ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BIOLOGICAL ASSAY; CHEMISTRY, ANALYTICAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEMOLYSIN PROTEINS; IMMUNOBLOTTING; LETHAL DOSE 50; MOTHS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

BACILLUS THURINGIENSIS; HEXAPODA; OSTRINIA NUBILALIS; ZEA MAYS;

EID: 37849010306     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01855-07     Document Type: Article

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