Detection of Cryptosporidium oocysts in fresh and frozen cattle faeces: Comparison of three methods

Letters in Applied Microbiology

Volumn 46, Issue 1, 2008, Pages 26-31

  Brook, E J ^a,b   Christley, R M ^b   French, N P ^c   Hart, C A ^b  

^a MOREDUN RESEARCH INSTITUTE   (United Kingdom)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

^c MASSEY UNIVERSITY   (New Zealand)

Author keywords

Cattle; Cryptosporidium; ROC; Sensitivity; Specificity

Indexed keywords

FARMS; SCREENING;

CATTLE; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM OOCYSTS; FAECAL SAMPLES; PERFORMANCE; ROC; SCREENING TESTS; SENSITIVITY; SENSITIVITY AND SPECIFICITY; SPECIFICITY;

POLYMERASE CHAIN REACTION;

AGRICULTURAL WASTES; ANIMALS; BIOASSAY; DIAGNOSIS; EPIDEMIOLOGY; FREEZING; SCREENING;

CATTLE; COMPARATIVE STUDY; CYST; DETECTION METHOD; FECES; PERFORMANCE ASSESSMENT; PROTOZOAN; TESTING METHOD;

ARTICLE; AURAMINE PHENOL STAIN; CATTLE; CRYPTOSPORIDIUM; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; ENZYME IMMUNOASSAY; FECES; GOLD STANDARD; INTERMETHOD COMPARISON; MICROORGANISM DETECTION; MODIFIED ZIEHL NEELSEN STAIN; NONHUMAN; OOCYST; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SCREENING TEST; SENSITIVITY AND SPECIFICITY; UNITED KINGDOM;

ENGLAND; EURASIA; EUROPE; UNITED KINGDOM; WESTERN EUROPE;

BOS; CRYPTOSPORIDIUM;

EID: 37649013791     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02257.x     Document Type: Article

References (35)

1. Aarnaes, S.L., Blanding, J., Speier, S., Forthal, D., de la Maza, L.M. Peterson, E.M. (1994) Comparison of the ProSpecT and Color Vue enzyme-linked immunoassays for the detection of Cryptosporidium in stool specimens. Diagn Microbiol Infect Dis 19, 221 225.
2. Baxby, D. Blundell, N. (1983) Sensitive, rapid, simple methods for detecting Cryptosporidium in faeces. Lancet 2, 1149.
3. Bialek, R., Binder, N., Dietz, K., Joachim, A., Knobloch, J. Zelck, U.E. (2002) Comparison of fluorescence, antigen and PCR assays to detect Cryptosporidium parvum in fecal specimens. Diagn Microbiol Infect Dis 43, 283 288.
4. Casemore, D.P. (1991) ACP Broadsheet 128: June 1991. Laboratory methods for diagnosing cryptosporidiosis. J Clin Pathol 44, 445 451.
5. Casemore, D.P., Armstrong, M. Sands, R.L. (1985) Laboratory diagnosis of cryptosporidiosis. J Clin Pathol 38, 1337 1341.
6. Castro-Hermida, J.A., Gonzalez-Losada, Y.A., Mezo-Menendez, M. Ares-Mazas, E. (2002a) A study of cryptosporidiosis in a cohort of neonatal calves. Vet Parasitol 106, 11 17.
7. Castro-Hermida, J.A., Gonzalez-Losada, Y.A. Ares-Mazas, E. (2002b) Prevalence of and risk factors involved in the spread of neonatal bovine cryptosporidiosis in Galicia (NW Spain). Vet Parasitol 106, 1 10.
8. Causer, L.M., Handzel, T., Welch, P., Carr, M., Culp, D., Lucht, R., Mudahar, K., Robinson, D. et al. (2006) An outbreak of Cryptosporidium hominis infection at an Illinois recreational waterpark. Epidemiol Infect 134, 147 156.
9. Conraths, F.J. Schares, G. (2006) Validation of molecular-diagnostic techniques in the parasitological laboratory. Vet Parasitol 136, 91 98.
10. Dohoo, I., Martin, W. Stryhn, H. (2003) Veterinary Epidemiologic Research Charlottestown, PE, Canada : Atlantic Veterinary College.
11. Garcia, L.S. Shimizu, R.Y. (1997) Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J Clin Microbiol 35, 1526 1529.
12. Garcia, L.S., Brewer, T.C. Bruckner, D.A. (1987) Fluorescence detection of Cryptosporidium oocysts in human fecal specimens by using monoclonal antibodies. J Clin Microbiol 25, 119 121.
13. Geurden, T., Berkvens, D., Geldhof, P., Vercruysse, J. Claerebout, E. (2006) A Bayesian approach for the evaluation of six diagnostic assays and the estimation of Cryptosporidium prevalence in dairy calves. Vet Res 37, 671 682.
14. Glaberman, S., Moore, J.E., Lowery, C.J., Chalmers, R.M., Sulaiman, I., Elwin, K., Rooney, P.J., Millar, B.C. et al. (2002) Three drinking-water- associated cryptosporidiosis outbreaks, Northern Ireland. Emerg Infect Dis 8, 631 633.
15. Ignatius, R., Eisenblatter, M., Regnath, T., Mansmann, U., Futh, U., Hahn, H. Wagner, J. (1997) Efficacy of different methods for detection of low Cryptosporidium parvum oocyst numbers or antigen concentrations in stool specimens. Eur J Clin Microbiol Infect Dis 16, 732 736.
16. Inoue, M., Uga, S., Oda, T., Rai, S.K., Vesey, G. Hotta, H. (2006) Changes of physical and biochemical properties of Cryptosporidium oocysts with various storage conditions. Water Res 40, 881 886.
17. Johnston, S.P., Ballard, M.M., Beach, M.J., Causer, L. Wilkins, P.P. (2003) Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens. J Clin Microbiol 41, 623 626.
18. Kehl, K.S., Cicirello, H. Havens, P.L. (1995) Comparison of four different methods for detection of Cryptosporidium species. J Clin Microbiol 33, 416 418.
19. MacKenzie, W.R., Hoxie, N.J., Proctor, M.E., Gradus, M.S., Blair, K.A., Peterson, D.E., Kazmierczak, J.J., Addiss, D.G. et al. (1994) A massive outbreak in Milwaukee of cryptosporidium infection transmitted through the public water supply. N Engl J Med 331, 161 167.
20. MacPherson, D.W. McQueen, R. (1993) Cryptosporidiosis: multiattribute evaluation of six diagnostic methods. J Clin Microbiol 31, 198 202.
21. Magi, B., Canocchi, V., Tordini, G., Cellesi, C. Barberi, A. (2006) Cryptosporidium infection: diagnostic techniques. Parasitol Res 98, 150 152.
22. Maldonado-Camargo, S., Atwill, E.R., Saltijeral-Oaxaca, J.A. Herrera-Alonso, L.C. (1998) Prevalence of and risk factors for shedding of Cryptosporidium parvum in Holstein Freisian dairy calves in central Mexico. Prev Vet Med 36, 95 107.
23. McCluskey, B.J., Greiner, E.C. Donovan, G.A. (1995) Patterns of Cryptosporidium oocyst shedding in calves and a comparison of two diagnostic methods. Vet Parasitol 60, 185 190.
24. Morgan, U.M., Pallant, L., Dwyer, B.W., Forbes, D.A., Rich, G. Thompson, R.C. (1998) Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial. J Clin Microbiol 36, 995 998.
25. Mtambo, M.M., Nash, A.S., Blewett, D.A. Wright, S. (1992) Comparison of staining and concentration techniques for detection of Cryptosporidium oocysts in cat faecal specimens. Vet Parasitol 45, 49 57.
26. Muccio, J.L., Grooms, D.L., Mansfield, L.S., Wise, A.G. Maes, R.K. (2004) Evaluation of two rapid assays for detecting Cryptosporidium parvum in calf feces. J Am Vet Med Assoc 225, 1090 1092.
27. Parisi, M.T. Tierno, P.M., Jr. (1995) Evaluation of new rapid commercial enzyme immunoassay for detection of Cryptosporidium oocysts in untreated stool specimens. J Clin Microbiol 33, 1963 1965.
28. Santin, M., Trout, J.M., Xiao, L., Zhou, L., Greiner, E. Fayer, R. (2004) Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Vet Parasitol 122, 103 117.
29. Siddons, C.A., Chapman, P.A. Rush, B.A. (1992) Evaluation of an enzyme immunoassay kit for detecting Cryptosporidium in faeces and environmental samples. J Clin Pathol 45, 479 482.
30. Sulaiman, I.M., Xiao, L. Lal, A.A. (1999) Evaluation of Cryptosporidium parvum genotyping techniques. Appl Environ Microbiol 65, 4431 4435.
31. Tee, G.H., Moody, A.H., Cooke, A.H. Chiodini, P.L. (1993) Comparison of techniques for detecting antigens of Giardia lamblia and Cryptosporidium parvum in faeces. J Clin Pathol 46, 555 558.
32. Ungar, B.L. (1990) Enzyme-linked immunoassay for detection of Cryptosporidium antigens in fecal specimens. J Clin Microbiol 28, 2491 2495.
33. Ward, L.A. Wang, Y. (2001) Rapid methods to isolate Cryptosporidium DNA from frozen feces for PCR. Diagn Microbiol Infect Dis 41, 37 42.
34. Weber, R., Bryan, R.T., Bishop, H.S., Wahlquist, S.P., Sullivan, J.J. Juranek, D.D. (1991) Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity of current diagnostic methods. J Clin Microbiol 29, 1323 1327.
35. Xiao, L., Escalante, L., Yang, C., Sulaiman, I., Escalante, A.A., Montali, R.J., Fayer, R. Lal, A.A. (1999) Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol 65, 1578 1583.