Electrotransfer of proteins in an environmentally friendly methanol-free transfer buffer

Analytical Biochemistry

Volumn 373, Issue 2, 2008, Pages 377-379

  Villanueva, Marco A ^a,b  

^a UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^b INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

METHANOL;

ELECTROTRANSFER; ENVIRONMENTALLY FRIENDLY CONDITION; IMMUNOBLOTTING; IMMUNODETECTION; ISO-PROPANOLS; POLYACRYLAMIDE GELS; POLYVINYLIDENE DIFLUORIDE; WESTERN BLOTS;

PROTEINS;

2 PROPANOL; ALCOHOL; BUFFER; FLUORIDE; IMMUNOGLOBULIN G; METHANOL; MOLECULAR MARKER; POLYACRYLAMIDE; POLYVINYLIDENE DIFLUORIDE; PYROXYLIN; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION (PARAMETERS); GEL; HEAVY CHAIN; IMMUNODETECTION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; RABBIT; SERUM; WESTERN BLOTTING;

ORYCTOLAGUS CUNICULUS;

EID: 37549014913     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.08.007     Document Type: Article

References (4)

1. Towbin J., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc. Natl. Acad. Sci. USA 76 (1979) 4350-4354
2. Burgress R.R. Mapping protein-protein interaction domains using ordered fragment ladder far-Western analysis of hexahistidine-tagged fusion proteins. Methods Enzymol. 328 (2000) 141-157
3. Mozdzanowski J., and Speicher D.W. Microsequence analysis of electroblotted proteins: I. Comparison of electroblotting recoveries using different types of PVDF membranes. Anal. Biochem. 207 (1992) 11-18
4. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685