Animal cell separation

Animal Cell Technology: From Biopharmaceuticals to Gene Therapy

Volumn , Issue , 2008, Pages 273-293

  Castilho, Leda dos Reis ^a   Medronho, Ricardo de Andrade ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 37549007767     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (45)

1. Avgerinos GC, Drapeau D, Socolow JS, Mao J, Hsiao K, Broeze RJ (1990), Spin filter perfusion system for high density cell culture: production of recombinant urinary type plasminogen activator in CHO cells, Bio/Technol. 8:54-58.
2. Belfort G, Davis RH, Zydney AL (1994), The behavior of suspensions and macromolecular solutions in cross-flow microfiltration, J. Membr. Sci. 96:1-58.
3. Björling T, Dudel U, Fenge C (1995), Evaluation of a cell separator in large scale perfusion culture, In: Beuvery EC, Griffiths JB, Zeijlemaker WP (Eds), Animal Cell Technology: Developments Towards the 21st Century, Kluwer Academic, Dordrecht, pp. 671-673.
4. Castilho LR, Anspach FB (2003), CFD-aided design of a dynamic filter for mammalian cell separation, Biotechnol. Bioeng. 83:514-524.
5. Castilho LR, Medronho RA (2000), A simple procedure for design and performance prediction of Bradley and Rietema hydrocyclones, Minerals Eng. 13:183-191.
6. Castilho LR, Medronho RA (2002), Cell retention devices for suspended-cell perfusion cultures, Adv. Biochem. Eng. Biotechnol. 74:129-169.
7. Castilho LR, Anspach FB, Deckwer W-D (2002), An integrated process for mammalian cell perfusion cultivation and product purification using a novel dynamic filter, Biotechnol. Progr. 18:776-781.
8. Centritech1 (2006), Product information. Available at: http://www.pneumaticscale. com/content/menus/psc/centritech_centrifuge.aspx (accessed March 2007).
9. Cheryan M (1998), Ultrafiltration and Microfiltration Handbook, Technomic Publishing, Lancaster.
10. Coelho MAZ, Medronho RA (2001), A model for performance prediction of hydrocyclones, Chem. Eng. J. 84:7-14.
11. Dalm MCF, Jansen M, Keijzer, TMP, Grunsven WMJ, Oudshoorn A, Tramper J, Martens DE (2005), Stable hybridoma cultivation in a pilot-scale acoustic perfusion system: long-term process performance and effect of recirculation rate, Biotechnol. Bioeng. 91:894-900.
12. Deckwer W-D, Anspach FB, Medronho RA, Luebberstedt M (2005), Method for separating viable cells from cell suspension, Patent US 6878545 B2.
13. Elsayed A, Piehl G-W, Nothnagel J, Medronho RA, Deckwer WD, Wagner R (2005), Use of hydrocyclone as an efficient tool for cell retention in perfusion cultures, In:Godia F, Fussenegger M (Eds), Animal Cell Technology Meets Genomics - Proceedings of the 18th ESACT Meeting (European Society for Animal Cell Technology), Kluwer Academic Publishers, Dordrecht, pp. 679-682.
14. Elsayed A, Medronho RA, Wagner R, Deckwer, W-D (2006), Use of hydrocyclones for mammalian cell retention - I. Separation efficiency and cell viability, Eng. Life Sci. 6:347-354.
15. Figueredo-Cardero A, Chico E, Castilho LR, Medronho RA (in press), CFD study of the fluid and particle dynamics in a spin-filter perfusion bioreactor, In: Hauser H (Ed.), Proceedings of the 20th ESACT Meeting (European Society for Animal Cell Technology), Springer, Dordrecht.
16. Gorenflo VM, Ritter JB, Aeschliman DS, Drouin H, Bowen BD, Piret JM (2005), Characterization and optimization of acoustic filter performance by experimental design methodology, Biotechnol. Bioeng. 90:746-753.
17. Gröschl M, Burger W, Handl B (1998), Ultrasonic separation of suspended particles - Part III: Application in biotechnology, Acustica 84:815-822.
18. Henzler H, Kauling J, Schmitt F, Beckers E, Boedeker B, Von Hugo H, Konstantinov K, Naveh D (2003), Continuous high cell density fermentation unit for cultivating suspended animal or plant cell lines uses sedimentation separator of specific surface area, Patent WO2003020919-A2.
19. Iding K, Lütkemeyer D, Fraune E, Gerlach K, Lehmann J (2000), Influence of alterations in culture condition and changes in perfusion parameters on the retention performance of a 20 _m spinfilter during a perfusion cultivation of a recombinant CHO cell line in pilot scale, Cytotechnology 34:141-150.
20. Jockwer A, Medronho RA, Wagner R, Anspach FB, Deckwer WD (2001), Use of hydrocyclones for mammalian cell retention in perfusion bioreactors, In: Olsson EL, Chatzissavidou N, Lüllau E (Eds), Animal Cell Technology: From Target to Market - Proceedings of the 17th ESACT Meeting (European Society for Animal Cell Technology), Kluwer Academic Publishing, Dordrecht, pp. 301-305.
21. Johnson M, Lantier S, Massie B, Lefebvre G, Kamen AA (1996), Use of the Centritech1 lab centrifuge for perfusion culture of hybridoma cells in protein-free medium, Biotechnol. Progr. 12:855-864.
22. Kempken R, Preissmann A, Berthold W (1995), Assessment of a disc stack centrifuge for use in mammalian-cell separation, Biotechnol. Bioeng. 46:132-138.
23. Kempken R, Rechtsteiner H, Schäfer J, Katz U, Dick O, Weidemeier R, Sellick I (1997), Dynamic membrane filtration in mammalian cell culture harvest, In:Carrondo MJT (Ed.), Animal Cell Technology, Kluwer, Dordrecht, pp. 379-382.
24. Kilburn DG, Clarke DJ, Coakley WT, Bardsley DW (1989), Enhanced sedimentation of mammalian cells following acoustic aggregation, Biotechnol. Bioeng. 34:559-562.
25. Klein E (1991), Affinity Membranes - Their Chemistry and Performance in Adsorptive Separation Processes, John Wiley and Sons, New York.
26. Luebberstedt M (2000), Separation tierischer Zellkulturen durch Einsatz von Hydrozyklonen, Diplomarbeit des Studienganges Biotechnologie der Technische Fachhochschule Berlin, Berlin.
27. Luebberstedt M, Medronho RA, Anspach FB, Deckwer W-D (2000), Abtrennung tierischer Zellen mit Hydrozyklonen, Chem. Ing. Tech. 72:1089-1090.
28. Medronho RA (2003), Solid-liquid separation, In: Mattiasson B, Hatti-Kaul R (Eds) Isolation and Purification of Proteins, Marcel Dekker, New York, pp. 131-190.
29. Medronho RA, Schütze J, Deckwer W-D (2005), Numerical simulation of hydrocyclones for cell separation, Latin Am. Appl. Res. 35:1-8.
30. Mercille S, Johnson M, Lemieux R, Massie B (1994), Filtration-based perfusion of hybridoma cultures in protein-free medium - reduction of membrane fouling by medium supplementation with DNAse-I, Biotechnol. Bioeng. 43:833-846.
31. Nobrega R, Borges CP, Habert AC (2005), Processos de separaçaõ com membranas, In: Pessoa A Jr, Kilikian BV (Eds), Purificaçaõ de Produtos Biotecnoló gicos, Editora Manole, Barueri/SP, pp. 37-88 (in Portuguese).
32. Pinto RCV, Medronho RA, Castilho LR (2007), The effects of cell separation with hydrocyclones on the viability of CHO cells, In: Smith R (Ed.), Cell Technology for Cell Products - Proceedings of the 19th Meeting of ESACT (European Society for Animal Cell Technology), Springer, Dordrecht, pp. 745-748.
33. Rautenbach R (1997), Membranverfahren - Grundlagen der Modul- und Anlagenauslegung, Springer Verlag, Berlin, pp. 60-77 (in German).
34. Roth G, Smith CE, Schoofs GM, Montgomery TJ, Ayala JL, Horwitz JI (1997), Using an external vortex flow filtration device for perfusion cell culture, BioPharm. 10:30-34.
35. Schulz C (1996), Einsatz eines Scherfilters zur Separation von Tierischen Zellen aus kontinuierlicher Kultivierung, Diplomarbeit des Studienganges Biotechnologie der Technischen Universität Carolo-Wihelmina, Braunschweig (in German).
36. Serra CA, Wiesner MR, Laine JM (1999), Rotating membrane disk filters: design evaluation using computational fluid dynamics, Chem. Eng. J. 72:1-5.
37. Shirgaonkar IZ, Lanthier S, Kamen A (2004), Acoustic cell filter: a proven cell retention technology for perfusion of animal cell cultures, Biotechnol. Adv. 22:433-444.
38. Silva CM, Reeve DW, Husain H, Rabie HR, Woodhouse KA (2000), Model for flux prediction in high-shear microfiltration systems, J. Membr. Sci. 173:87-98.
39. Smith CG, Guillaume JM, Greenfield PF, Randerson DH (1991), Experience in scaleup of homogeneous perfusion culture for hybridomas, Bioproc. Eng. 6:213-219.
40. Svarovsky L (2000), Gravity clarification and thickening, In: Svarovsky L (ed), Solid-Liquid Separation, Butterworth-Heinemann, Oxford.
41. Takamatsu H, Hamamoto K, Ishimaru K, Yokoyama S, Tokashiki M (1996), Largescale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones, Appl. Microbiol. Biotechnol. 45:454-457.
42. Tebe H, Lütkemeyer D, Gudermann F, Heidemann R, Lehmann J (1997), Lysis-free separation of hybridoma cells by continuous disc stack centrifugation, Cytotechnology 22:119-127.
43. Vogel JH, Kroner KH (1999), Controlled shear filtration: a novel technique for animal cell separation, Biotechnol. Bioeng. 63:663-674.
44. Woodside SM, Bowen BD, Piret JM (1998), Mammalian cell retention devices for stirred perfusion systems, Cytotechnology 28:163-175.
45. Zhang S, Handa-Corrigan A, Spier RE (1993), A comparison of oxygenation methods for high-density perfusion cultures of animal cells, Biotechnol. Bioeng. 41:685-692.