Structural and mechanistic analysis of trichodiene synthase using site-directed mutagenesis: Probing the catalytic function of tyrosine-295 and the asparagine-225/serine-229/glutamate-233-Mg2 +B motif

Archives of Biochemistry and Biophysics

Volumn 469, Issue 2, 2008, Pages 184-194

  Vedula, L Sangeetha ^a   Jiang, Jiaoyang ^b   Zakharian, Tatiana ^a   Cane, David E ^b   Christianson, David W ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b BROWN UNIVERSITY   (United States)

Author keywords

Enzyme kinetics; Farnesyl diphosphate; Protein crystallography; Terpenoid cyclase

Indexed keywords

ASPARAGINE; ENZYME; GLUTAMIC ACID; MAGNESIUM; SERINE; SESQUITERPENE DERIVATIVE; TRICHODIENE SYNTHASE; TYROSINE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CATALYSIS; DEGRADATION KINETICS; DRUG STRUCTURE; ENZYME ANALYSIS; PRIORITY JOURNAL; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS; STEADY STATE; WILD TYPE; X RAY CRYSTALLOGRAPHY;

AMINO ACID MOTIFS; ASPARAGINE; BIOCHEMISTRY; CARBON-CARBON LYASES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; FUSARIUM; GLUTAMIC ACID; KINETICS; MAGNESIUM; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; SERINE; TYROSINE;

FUSARIUM SPOROTRICHIOIDES;

EID: 37349024660     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2007.10.015     Document Type: Article

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