Fabrication of protein A-viologen hetero Langmuir-Blodgett film for fluorescence immunoassay

Biotechnology and Bioprocess Engineering

Volumn 9, Issue 4, 2004, Pages 241-244

  Lee, Woochang ^a   Chun, Bum Suk ^a   Oh, Byung Keun ^a   Lee, Won Hong ^a   Choi, Jeong Woo ^a  

^a Sogang University   (South Korea)

Author keywords

Antibody immobilization; Langmuir Blodgett film; Protein A; Viologen

Indexed keywords

EID: 37249008952     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02942337     Document Type: Article

References (14)

1. Choi, J. W., Y. S. Nam, and W. H. Lee (2004) Nanoscale fabrication of biomolecular layer and its application to biodevices. Biotechnol. Bioprocess Eng. 9: 76-85.
2. Oh, B.-K., W. Lee, W. H. Lee, and J.-W. Choi (2003) Nano-scale probe fabrication using self-assembly technique and application to detection of Escherichia coli 0157:H7. Biotechnol. Bioprocess Eng. 8: 227-232.
3. Oh, B. K., W. Lee, Y. M. Bae, W. H. Lee, J. W. Choi (2003) Surface plasmon resonance immunosensor for detection of Legionella pneumophila. Biotechnol. Bioprocess Eng. 8: 112-116.
4. Turko, I. V., I. S. Yurkevich, and V. L. Chashchin (1995) Oriented immunoglobulin G layer onto the Langmuir-Blodgett films of protein A. Thin Solid Films. 210/211: 710-712.
5. Dubrovsky, T., A. Tronin, S. Dubrovskaya, S. Vakula, and C. Nicolini (1995) Immunological activity of IgG Langmuir films oriented by protein A sublayer. Sensor Actuat. B-Chem. 23: 1-7.
6. Ulman, A. (1991) An Introduction to Ultrathin Organic Films from Langmuir-Blodgett to Self-Assembly, pp. 101-236. Academic Press Inc., NY, USA.
7. Chudinova, G. K., A. V. Chudinov, V. V. Savransky, and A. M. Prokhorov (1997) The study of the antigen-antibody reaction by fluorescence method in LB films for immunosensor. Thin Solid Films 307: 294-297.
8. Choi, H. G., B. K. Oh, W. H. Lee, and J. W. Chio (2001) Deposition behavior and photoelectrochemical characteristics of chlorophyll a Langmuir-Blodgett films. Biotehcnol. Bioprocess Eng. 6: 183-188.
9. Choi, J. W., J. H. Park, W. Lee, B. K. Oh, J. Min, and W. H. Lee (2001) Fluorescence Immunoassay of HDL and LDL using Protein A LB film. J. Microbiol. Biotechnol. 11: 979-985.
10. Tundo, P., D. J. Kippenberger, M. J. Politi, P. Klahn, J.H. Fendler (1982) Redox active functionally polymerized surfactant vesicles synthesis and characterization. J. Am. Chem.Soc. 104:5352-5358.
11. Bollag, D. M., M. D. Rozycki, and S. J. Edelstein (1996) Protein Methods. 2nd ed., pp. 57-82. John Wiley, NY, USA.
12. Kiselyova, O. I., I. V. Yaminsky, Y. D. Ivanov, I. P. Kanaeva, V. Y. Kuznetsov, and A. I. Archakov (1999) AFM study of membrane proteins, cytochrome P450 2B4, and NADPH-cytochrome P450 reductase and their complex formation. Arch. Biochem. Biophys. 371: 1-7.
13. Hayes, J. J., M. Kenmore, A. Badley, and D. C. Cullen (1998) AFM study of antibody adsorption to polystylene microtiter plates. Nanobiology 4: 141-151.
14. Coen, M. C, R. Lehmann, P. Croning, M. Bielmann, C. Galli, and L. Schlapbach (2001) Adsorption and bioactivity of protein A on silicon surfaces studied by AFM and XPS. J. Colloid Interf Sci. 233: 180-189.