Construction and expression of single-chain antibody derived from a new clone of monoclonal antibody against human CD14 in CHO Cells

Immunopharmacology and Immunotoxicology

Volumn 29, Issue 3-4, 2007, Pages 375-386

  Tang, Yong Min ^a   Ning, Bo Tao ^a   Cao, Jiang ^b   Shen, Hong Qiang ^a   Qian, Bai Qin ^a  

^a ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

CD14; Chinese hamster ovary; Eukaryotic; Expression; ScFv

Indexed keywords

CD14 ANTIGEN; IMMUNOTOXIN; MONOCLONAL ANTIBODY; PRIMER DNA; SERINE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TAQ POLYMERASE; UNCLASSIFIED DRUG; ZHEJIANG CHILDREN HOSPITAL 7 2F9;

ANIMAL CELL; ANTIBODY ENGINEERING; ANTIGEN EXPRESSION; ARTICLE; BINDING AFFINITY; BIOLOGICAL ACTIVITY; CELL TRANSFORMATION; CHO CELL; CONTROLLED STUDY; DNA SYNTHESIS; EUKARYOTE; EUKARYOTIC CELL; EXPRESSION VECTOR; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; GENE AMPLIFICATION; GENE IDENTIFICATION; GENE SEQUENCE; GENE VECTOR; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC TRANSFECTION; MOLECULAR CLONING; MOLECULAR WEIGHT; MOUSE; MULTIGENE FAMILY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; RECOMBINANT PLASMID; RNA SPLICING; SUPERNATANT; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, BLOCKING; ANTIBODIES, MONOCLONAL; ANTIGENS, CD14; BASE SEQUENCE; BLOTTING, WESTERN; CELL LINE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; HUMANS; INDICATORS AND REAGENTS; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN ENGINEERING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

CRICETULUS GRISEUS; EUKARYOTA;

EID: 37149025674     PISSN: 08923973     EISSN: 15322513     Source Type: Journal    
DOI: 10.1080/08923970701619927     Document Type: Article

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