Isolation and characterization of μ-calpain, m-calpain, and calpastatin from postmortem muscle. I. Initial steps

Journal of Animal Science

Volumn 85, Issue 12, 2007, Pages 3400-3414

  Camou, J P ^a,b   Mares, S W ^a   Marchello, J A ^a   Vazquez, R ^a   Taylor, M ^a   Thompson, V F ^a   Goll, D E ^a  

^a UNIVERSITY OF ARIZONA   (United States)

^b CENTRO DE INVESTIGACIÓN EN ALIMENTACIÓN Y DESARROLLO   (Mexico)

Author keywords

calpain; Calpastatin; m Calpain; Postmortem muscle; Protein purification

Indexed keywords

CALCIUM BINDING PROTEIN; CALPAIN; CALPAIN 1; CALPAIN 2; CALPASTATIN; M-CALPAIN; MU-CALPAIN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CADAVER; CATTLE; CHEMISTRY; ENZYMOLOGY; FOOD HANDLING; FOOD PRESERVATION; ISOLATION AND PURIFICATION; MEAT; METABOLISM; METHODOLOGY; MOLECULAR WEIGHT; PHYSIOLOGY; SKELETAL MUSCLE; STANDARD; TIME;

ANIMALS; CALCIUM-BINDING PROTEINS; CALPAIN; CATTLE; FOOD HANDLING; FOOD PRESERVATION; MEAT; MOLECULAR WEIGHT; MUSCLE, SKELETAL; POSTMORTEM CHANGES; TIME FACTORS;

BOVINAE;

EID: 37049025981     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/jas.2007-0356     Document Type: Article

References (32)

1. Arthur, J. S. C., and D. L. Mykles. 2000. Calpain zymography with casein or fluorescein isothiocyanate casein. Pages 109-116 in Methods in Molecular Biology, vol. 144: Calpain Methods and Protocols. J. S. Elce, ed. Humana Press Inc., Totowa, NJ.
2. Boehm, M. L., T. L. Kendall, V. F. Thompson, and D. E. Goll. 1998. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. J. Anim. Sci. 76:2415-2434.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72:248-254.
4. Camou, J. P., J. A. Marchello, V. F. Thompson, S. W. Mares, and D. E. Goll. 2007. Effect of postmortem storage on activity of μ-and m-calpain in five different bovine muscles. J. Anim. Sci. 85:2660-2667.
5. Delgado, E. F., G. H. Geesink, J. A. Marchello, D. E. Goll, and M. Koohmaraie. 2001. The calpain system in three muscles of normal and callipyge sheep. J. Anim. Sci. 79:398-412.
6. Ducastaing, A., C. Valin, J. Schollmeyer, and R. Cross. 1985. Effects of electrical stimulation on post-mortem changes in the activities of two Ca dependent neutral proteinases and their inhibitor in beef muscle. Meat Sci. 15:193-202.
7. Edmunds, T., P. A. Nagainis, S. K. Sathe, V. F. Thompson, and D. E. Goll. 1991. Comparison of the autolyzed and unautolyzed forms of μ- and m-calpain from bovine skeletal muscle. Biochim. Biophys. Acta 1077:197-208.
8. Geesink, G. H., and M. Koohmaraie. 1999. Effect of calpastatin on degradation of myofibrillar proteins by μ-calpain under postmortem conditions. J. Anim. Sci. 77:2685-2692.
9. Geesink, G. H., and M. Koohmaraie. 2000. Ionic strength-induced inactivation of μ-calpain in postmortem muscle. J. Anim. Sci. 78:2336-2343.
10. Geesink, G. H., S. Kuchay, A. H. Chirhti, and M. Koohmaraie. 2006. μ-Calpain is essential for postmortem proteolysis of muscle proteins. J. Anim. Sci. 84:2834-2840.
11. Goll, D. E., R. G. Taylor, J. A. Christiansen, and V. F. Thompson. 1992. Role of proteinases and protein turnover in muscle growth and meat quality. Pages 25-36 in Proc. 44th Annu. Recip. Meat Conf., Natl. Live Stock and Meat Board, Chicago, IL.
12. Goll, D. E., V. F. Thompson, H. Li, W. Wei, and J. Cong. 2003. The calpain system. Physiol. Rev. 83:731-801.
13. Karlsson, J.-O., S. Gustavsson, C. Hall, and E. Nilsson. 1985. A simple one-step procedure for separation, of calpain I, calpain II, and calpastatin. Biochem. J. 231:201-204.
14. Kent, M. P., M. J. Spencer, and M. Koohmaraie. 2004. Postmortem proteolysis is reduced in transgenic mice overexpressing calpastatin. J. Anim. Sci. 82:794-801.
15. Koohmaraie, M. 1988. The role of endogenous proteases in meat tenderness. Pages 89-100 in Proc. 41st Annu. Recip. Meat Conf., Natl. Live Stock and Meat Board, Chicago, IL. Natl. Live Stock and Meat Board, Chicago, IL.
16. Koohmaraie, M. 1992. The role of Ca2+-dependent proteinases (calpains) in post-mortem proteolysis and meat tenderness. Biochimie 74:239-245.
17. Koohmaraie, M. 1996. Biochemical factors regulating the toughening and tenderization process of meat. Meat Sci. 43:193-201.
18. Koohmaraie, M., S. C. Seideman, J. E. Schollmeyer, T. R. Dutson, and J. D. Grouse. 1987. Effect of postmortem storage on Ca-dependent proteases, their inhibitor and myofibril fragmentation. Meat Sci. 19:187-196.
19. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
20. Li, H., V. F. Thompson, and D. E. Goll. 2004. Effects of autolysis on properties of μ- and m-calpain. Biochim. Biophys. Acta 1691:91-103.
21. Talbot, P. J., R. L. Knobler, and M. J. Buchmeier. 1984. Western and dot immunoblotting analysis of viral antigens and antibodies: Application to murine hepatitis virus. J. Immunol. Meth. 73:177-188.
22. Tan, F. C., D. E. Goll, and Y. Otsuka. 1988. Some properties of the millimolar Ca2+-dependent proteinase from bovine cardiac muscle. J. Mol. Cell. Cardiol. 20:983-997.
23. Taylor, R. G., G. H. Geesink, V. F. Thompson, M. Koohmaraie, and D. E. Goll. 1995. Is Z-disk degradation responsible for postmortem tenderization? J. Anim. Sci. 73:1351-1367.
24. Thompson, V. F., and D. E. Goll. 2000. Purification of μ-calpain, m-calpain, and calpastatin from animal tissues. Pages 3-16 in Methods in Molecular Biology, vol. 144: Calpain Methods and Protocols. J. S. Elce, ed. Humana Press Inc., Totowa, NJ.
25. Thompson, V. F., S. Saldana, J. Cong, and D. E. Goll. 2000. A BODIPY fluorescent microplate assay for measuring activity of calpains and other proteases. Anal. Chem. 279:170-178.
26. Thompson, V. F., S. Saldaña, J. Cong, D. M. Luedke, and D. E. Goll. 2002. The calpain system in human placenta. Life Sci. 70:2493-2508.
27. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
28. Veiseth, E., S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie. 2001. Effect of postmortem storage on μ-calpain and m-calpain in ovine skeletal muscle. J. Anim. Sci. 79:1502-1508.
29. Veiseth, E., S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie. 2004. Indicators of tenderization are detectable by 12 h postmortem in ovine muscle. J. Anim. Sci. 82:1428-1436.
30. Vidalenc, P., P. Cottin, N. Merdaci, and A. Ducastaing. 1983. Stability of two Ca2+-dependent neutral proteinases and their specific inhibitor during post-mortem storage of rabbit skeletal muscle. J. Sci. Food Agric. 34:1241-1250.
31. Wendt, A., V. F. Thompson, and D. E. Goll. 2004. Interaction of calpain with calpastatin: A review. Biol. Chem. 385:465-472.
32. Wolfe, F. H., S. K. Sathe, D. E. Goll, W. C. Kleese, T. Edmunds, and S. M. Duperret. 1989. Chicken skeletal muscle has three Ca2+-dependent proteinases. Biochim. Biophys. Acta 998:236-250.