Pyrene-functionalized 2′-amino-α-L-LNA as potential diagnostic probes

Nucleosides, Nucleotides and Nucleic Acids

Volumn 26, Issue 10-12, 2007, Pages 1407-1409

  Kumar, T Santhosh ^a   Wengel, Jesper ^a   Hrdlicka, Patrick J ^b  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b UNIVERSITY OF IDAHO   (United States)

Author keywords

2 amino L LNA; Abasic sites; Pyrene; Single nucleotide polymorphisms

Indexed keywords

LOCKED NUCLEIC ACID; OLIGODEOXYRIBONUCLEOTIDE; PYRENE;

ARTICLE; BASE MISPAIRING; CONFORMATIONAL TRANSITION; DIAGNOSTIC PROCEDURE; DNA DETERMINATION; RNA STABILITY; SINGLE NUCLEOTIDE POLYMORPHISM;

ANIMALS; FLUORESCENT DYES; HUMANS; OLIGONUCLEOTIDES; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; THYMIDINE MONOPHOSPHATE;

EID: 36949009234     PISSN: 15257770     EISSN: 15322335     Source Type: Journal    
DOI: 10.1080/15257770701538908     Document Type: Article

References (7)

1. a) Okamoto, A.; Kanatani, K.; Saito, I. Pyrene-labeled base-discriminating fluorescent DNA probes for homogeneous SNP typing. J. Am. Chem. Soc. 2004, 126, 4820-4827;
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3. a) Matray, T.J.; Kool, E.T. Selective and stable DNA base pairing without hydrogen bonds. J. Am. Chem. Soc. 1998, 120, 6191-6192;
4. b) Langenegger, S.M.; Häner, R. Remarkable stabilization of duplex DNA containing an abasic site by non-nucleosidic phenanthroline and pyrene building blocks. Chem. Bio. Chem. 2005, 6, 848-851;
5. c) Valis, L.; Amann, N.; Wagenknecht, H. Detection of single base mismatches and abasic sites using phenanthridinium as an artificial DNA base and charge donor. Org. Biomolec. Chem. 2005, 3, 36-38.
6. Hrdlicka, P.J.; Kumar, T.S.; Wengel, J. Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2′-amino-α-L-LNA. Chem. Comm. 2005, 4279-4281.
7. Full experimental details will be published elsewhere.