Stabilization of multi-stranded nucleic acid structures using 3′-S-phosphorothiolate linkages

Nucleosides, Nucleotides and Nucleic Acids

Volumn 26, Issue 6-7, 2007, Pages 555-558

  Cosstick, Richard ^a   Buckingham, Joanne ^a   Brazier, John ^a   Fisher, Julie ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

i motif; Oligonucleotide; Phosphorothiolate

Indexed keywords

COMPLEMENTARY DNA; COMPLEMENTARY RNA; DOUBLE STRANDED DNA; NUCLEIC ACID; PHOSPHOROTHIOIC ACID DERIVATIVE;

CONFERENCE PAPER; DNA STRAND; DNA STRUCTURE; MOLECULAR STABILITY; NUCLEIC ACID STRUCTURE; PROTEIN MOTIF; RNA STABILITY; RNA STRUCTURE;

NUCLEIC ACID CONFORMATION; NUCLEIC ACID HETERODUPLEXES; NUCLEIC ACIDS; OLIGONUCLEOTIDES; PHOSPHATES;

EID: 36948999831     PISSN: 15257770     EISSN: 15322335     Source Type: Journal    
DOI: 10.1080/15257770701490001     Document Type: Conference Paper

References (5)

1. Tereshko, V.; Gryaznov, S.; Egli, M. Consequences of replacing the DNA 3′-oxygen by an amino group: High-resolution crystal structure of a fully modified N3′-> P5′-phosphoramidate DNA dodecamer duplex. J. Am. Chem. Soc. 1998, 120(2), 269-283.
2. Sabbagh, G.; Fettes, K.J.; Gosain, R.; O'Neil, I.A.; Cosstick, R. Synthesis of phosphorothioamidites derived from 3′-thio-3′- deoxythymidine and 3′-thio-2′,3′-dideoxycytidine and the automated synthesis of oligodeoxynucleotides containing a 3′-S- phosphorothiolate linkage. Nucl. Acids Res. 2004, 32(2), 495-501.
3. For most recent example see: Elliott, S.L.; Brazier, J.; Cosstick, R.; Connolly, B.A. Mechanism of the Escherichia coli DNA T:G-mismatch endonuclease (Vsr protein) probed with thiophosphate-containing oligodeoxynucleotides, J. Mol. Biol. 2005, 353(3), 692-703.
4. Beevers, A.P.G.; Fettes, K.J.; Sabbagh, G.; Murad, F.K.; Arnold, J.R.P.; Cosstick, R.; Fisher, J. NMR and UV studies of 3′-S-phosphorothiolate modified DNA in a DNA: RNA hybrid dodecamer duplex; implications for antisense drug design. Org. Biomol. Chem. 2004, 2(1), 114-119.
5. Brazier JA, Fisher J, Cosstick RStabilization of the DNA I-motif structure by incorporation of 3′-S-phosphorothiolate linkages. Angew Chemie. Int. Ed. 2006, 45 (1), 114-117.