A novel cold-adapted phospholipase A1 from Serratia sp. xjF1: Gene cloning, expression and characterization

Enzyme and Microbial Technology

Volumn 42, Issue 2, 2008, Pages 187-194

  Fu, Jianhong ^a,b   Huang, Huoqing ^c   Meng, Kun ^c   Yuan, Tiezheng ^c   Yao, Bin ^c   Shi, Yuhu ^a,b   Ouyang, Pingkai ^a  

^a NANJING TECH UNIVERSITY   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Characterization; Cold adapted phospholipase A1; Expression; Serratia sp.

Indexed keywords

CLONING; ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; MOLECULAR WEIGHT;

COLD-ADAPTED PHOSPHOLIPASE; GENE ISOLATION; PICHIA PASTORIS;

ENZYME ACTIVITY;

AMINO ACID; CALCIUM; GENE PRODUCT; PHOSPHOLIPASE A1;

AMINO ACID SEQUENCE; ARTICLE; BACTERIUM ISOLATION; BIOREACTOR; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC CODE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY; SERRATIA; SERRATIA LIQUEFACIENS; SERRATIA MARCESCENS; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PICHIA PASTORIS; SERRATIA MARCESCENS; SERRATIA SP.;

EID: 36849092934     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2007.09.004     Document Type: Article

References (28)

1. Alquati C., De Gioia L., Santarossa G., Alberghina L., Fantucci P., and Lotti M. The cold-active lipase of Pseudomonas fragi heterologous expression, biochemical characterization and molecular modeling. Eur J Biochem 269 (2002) 3321-3328
2. 1 from Aspergillus oryzae. Biosci Biotechnol Biochem 63 (1999) 820-826
3. 1 from bovine brain. J Biol Chem 269 (1994) 19494-19500
4. 1 from Serratia sp. MK1 in Escherichia coli. J Biotechnol 72 (1999) 103-114
5. Li Q., Yang J., Yang B., Lin W., and Yu R. Vegetable oil degumming by different phospholipase. Chin Lipid 29 (2004) 19-22
6. Yang B., and Wang H. Economical and environment-friendly technique of enzymatic degumming. Chin Lipid 29 (2004) 21-23
7. Yang J., Yang B., Li Q., and Lin W. Rapeseed oil degumming by Lecitase Ultra. Chin Lipid 28 (2003) 31-34
8. Yang J., Yang B., Meng J., Li Q., and Lin W. Soybean oil degumming by a novel phospholipase-Lecitase Ultra. Chin Lipid 28 (2003) 10-13
9. 1 from Serratia sp. MK1. J Microbiol Biotechnol 6 (1996) 407-413
10. 1 suitable for large-scale production. J Appl Microbiol 86 (1999) 226-230
11. Homma H., Chiba N., Kobayashi T., Kudo I., Inoue K., Ikeda H., et al. Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. J Biochem 96 (1984) 1645-1653
12. 1 purified from Escherichia coli. Biochemistry 10 (1971) 4447-4456
13. Keen N.T., Ridgway D., and Boyd C. Cloning and characterization of a phospholipase gene from Erwinia chrysanthemi EC16. Mol Microbiol 6 (1992) 179-187
14. 1 from Serratia liquefaciens. J Bacteriol 170 (1988) 5855-5862
15. 1 cDNA from Arabidopsis thaliana. Eur J Biochem 271 (2004) 3752-3764
16. Kim M.K., Kim J.K., and Rhee J.S. Isolation of a phospholipase A1-producing microorganism. J Ind Microbiol Biotechnol 16 (1996) 171-174
17. Liu Y.G., and Whittier R.F. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25 (1995) 674-681
18. Liu Y.G., Mitsukawa N., Oosumi T., and Whittier R.F. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8 (1995) 457-463
19. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
20. Ramchuran S.O., Vargas V.A., Hatti-Kaul R., and Karlsson E.N. Production of a lipolytic enzyme originating from Bacillus halodurans LBB2 in the methylotrophic yeast Pichia pastoris. Appl Microbiol Biotechnol 71 (2006) 463-472
21. Abdou A.M. Purification and partial characterization of psychrotrophic Serratia marcescens lipase. J Dairy Sci 86 (2003) 127-132
22. Choo D.W., Kurihara T., Suzuki T., Soda K., and Esaki N. A cold-adapted lipase of an alaskan psychrotroph, Pseudomonas sp. strain B11-1: gene cloning and enzyme purification and characterization. Appl Environ Microbiol 64 (1998) 486-491
23. Rashid N., Shimada Y., Ezaki S., Atomi H., and Imanaka T. Low-temperature lipase from psychrotrophic Pseudomonas sp. strain KB700A. Appl Environ Microbiol 67 (2001) 4064-4069
24. Givskov M., and Molin S. Secretion of Serratia liquefaciens phospholipase from Escherichia coli. Mol Microbiol 8 (1993) 229-242
25. Givskov M., Eberl L., Christiansen G., Benedik M.J., and Molin S. Induction of phospholipase-and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flageller master operon flhD. Mol Microbiol 15 (1995) 445-454
26. Givskov M., and Molin S. Expression of extracellular phospholipase from Serratia liquefaciens is growth-phase-dependent, catabolite-repressed and regulated by anaerobiosis. Mol Microbiol 6 (1992) 1363-1374
27. Hochacha P.W., and Somero G.N. Biochemical adaption (1984), Princeton University Press, New Jersey, USA pp. 377-422
28. Feller G., and Gerday C. Psychrophilic enzymes: molecular basis of cold adaption. Cell Mol Life Sci 53 (1997) 830-841