Construction of internal control for the quantitative assay of Aspergillus fumigatus using real-time nucleic acid sequence-based amplification

Diagnostic Microbiology and Infectious Disease

Volumn 60, Issue 1, 2008, Pages 121-124

  Yoo, Jin Hong ^a   Choi, Su Mi ^a   Choi, Jung Hyun ^a   Kwon, Eun Young ^a   Park, Chulmin ^a   Shin, Wan Shik ^a  

^a The Catholic University of Korea College of Medicine   (South Korea)

Author keywords

Aspergillus; Internal control; NASBA

Indexed keywords

6 CARBOXYFLUORESCEIN; RNA;

ARTICLE; ASPERGILLUS FUMIGATUS; CONTROLLED STUDY; FUNGAL DETECTION; GENE AMPLIFICATION; IN VITRO STUDY; NONHUMAN; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION;

ACTINOMYCETALES; ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; BACTERIOPHAGE T7; HUMANS; PROMOTER REGIONS (GENETICS); REFERENCE STANDARDS; SELF-SUSTAINED SEQUENCE REPLICATION;

EID: 36849026044     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2007.08.001     Document Type: Article

References (15)

1. Bland J.M., and Altman D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1 (1986) 307-310
2. Compton J. Nucleic acid sequence-based amplification. Nature 350 (1991) 91-92
3. Damen M., Sillekens P., Cuypers H.T., Frantzen I., and Melsert R. Characterization of the quantitative HCV NASBA assay. J. Virol. Methods 82 (1999) 45-54
4. Ginocchio G.C., Kemper M., Stellrecht K.A., and Witt D.J. Multicenter evaluation of the performance characteristics of the NucliSens HIV-1 QT assay used for quantitation of human immunodeficiency virus type 1 RNA. J. Clin. Microbiol. 41 (2003) 164-173
5. Greijer A.E., Adriaanse H.M.A., Dekkers C.A.J., and Middeldorp J.M. Multiplex real-time NASBa for monitoring expression dynamics of human cytomegalovirus encoded IE1 and pp67 RNA. J. Clin. Virol. 24 (2002) 57-66
6. Kalendar R. FastPCR: a PCR primer design and repeat sequence searching software with additional tools for the manipulation and analysis of DNA and protein. [Online] (2005). http://www.biocenter.helsinki.fi/bi/programs/fastpcr.htm (Accessed 07 August 2006)
7. Loeffler J., Hebart H., Cox P., Flues N., Schumacher U., and Einsele H. Nucleic acid sequence-based amplification of aspergillus RNA in blood samples. J. Clin. Microbiol. 39 (2001) 1626-1629
8. Loens K., Ursi D., Ieven M., van Aarle P., Sillekens P., Oudshoorn P., et al. Detection of Mycoplasma pneumoniae in spiked clinical samples by nucleic acid sequence-based amplification. J. Clin. Microbiol. 40 (2002) 1339-1345
9. Patterson S.S., Casper E.T., Garcia-Rubio L., and Smith M.C. Increased precision of microbial RNA quantification using NASBA with an internal control. J. Microbiol. Methods 60 (2005) 343-352
10. Rodríguez-Lázaro D.M., Pla M., and Cook N. Construction strategy for an internal amplification control for real-time diagnostic assays using nucleic acid sequence-based amplification: development and clinical application. J. Clin. Microbiol. 42 (2004) 5832-5836
11. Vallone P.M., and Butler J.M. AutoDimer: a screening tool for primer-dimer and hairpin structures. BioTechniques 37 (2004) 226-231
12. Yeo S.I., and Wong B. Current status of non-culture methods for diagnosis of invasive fungal infections. Clin. Microbiol. Rev. 15 (2002) 465-484
13. Yoo J.H., Choi J.H., Choi S.M., Lee D.G., Shin W.S., Min W.S., and Kim C.C. Application of nucleic acid sequence-based amplification for diagnosis of and monitoring the clinical course of invasive aspergillosis in patients with hematologic diseases. Clin. Infect. Dis. 40 (2005) 392-398
14. Yoo J.H., Choi S.M., Lee D.G., Park S.H., Choi J.H., Kwon E.Y., and Shin W.S. Comparison of the real-time nucleic acid sequence-based amplification (RTi-NASBA) with conventional NASBA, and galactomannan assay for the diagnosis of invasive aspergillosis. J. Korean Med. Sci. 22 (2007) 672-676
15. Zuker M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31 (2003) 3406-3415