Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF

FEMS Microbiology Letters

Volumn 278, Issue 1, 2008, Pages 121-127

  Gopalaswamy, Radha ^a,b,c   Narayanan, Sujatha ^a   Jacobs Jr , William R ^c   Av Gay, Yossef ^b  

^a NATIONAL INSTITUTE FOR RESEARCH IN TUBERCULOSIS   (India)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Biofilm; Cell morphology; GPLs; Mycobacteria; Serine threonine kinase; Sliding motility

Indexed keywords

LIPID; PROTEIN KINASE; SERINE; THREONINE PROTEIN KINASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM COLONY; BIOFILM; CELL STRUCTURE; CONTROLLED STUDY; LIPID COMPOSITION; MICROBIAL ACTIVITY; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL;

BIOFILMS; CLONING, MOLECULAR; MICROSCOPY, ELECTRON, SCANNING; MOVEMENT; MYCOBACTERIUM SMEGMATIS; PROTEIN-SERINE-THREONINE KINASES; SEQUENCE ALIGNMENT;

CORYNEBACTERINEAE; EUKARYOTA; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS;

EID: 36749061398     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2007.00989.x     Document Type: Article

References (20)

1. Billman-Jacobe H, McConville MJ, Haites RE, Kovacevic S Coppel RL (1999) Identification of a peptide synthetase involved in the biosynthesis of glycopeptidolipids of Mycobacterium smegmatis. Mol Microbiol 33 : 1244 1253.
2. Cole ST, Brosch R, Parkhill J et al 1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393 : 537 544.
3. Cozzone AJ (2005) Role of protein phosphorylation on serine/threonine and tyrosine in the virulence of bacterial pathogens. J Mol Microbiol Biotechnol 9 : 198 213.
4. Daugelat S, Kowall J, Mattow J, Bumann D, Winter R, Hurwitz R Kaufmann SH (2003) The RD1 proteins of Mycobacterium tuberculosis: expression in Mycobacterium smegmatis and biochemical characterization. Microbes Infect 5 : 1082 1095.
5. Deol P, Vohra R, Saini AK et al 2005) Role of Mycobacterium tuberculosis Ser/Thr Kinase PknF: implications in glucose transport and cell division. J Bacteriol 187 : 3415 3420.
6. Deshayes C, Laval F, Montrozier H, Daffe M, Etienne G Reyrat JM (2005) A glycosyltransferase involved in biosynthesis of triglycosylated glycopeptidolipids in Mycobacterium smegmatis: impact on surface properties. J Bacteriol 187 : 7283 7291.
7. Etienne G, Villeneuve C, Billman-Jacobe H, Astarie-Dequeker C, Dupont MA Daffe M (2002) The impact of the absence of glycopeptidolipids on the ultrastructure, cell surface and cell wall properties, and phagocytosis of Mycobacterium smegmatis. Microbiology 148 : 3089 3100.
8. Etienne G, Laval F, Villeneuve C et al 2005) The cell envelope structure and properties of Mycobacterium smegmatis mc(2)155: is there a clue for the unique transformability of the strain? Microbiology 151 : 2075 2086.
9. Grundner C, Gay LM Alber T (2005) Mycobacterium tuberculosis serine/threonine kinases PknB, PknD, PknE, and PknF phosphorylate multiple FHA domains. Protein Sci 14 : 1918 1921.
10. Hanks SK, Quinn AM Hunter T (1988) The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241 : 42 52.
11. Hatfull GF Jacobs WR Jr. (2000) Molecular Genetics of Mycobacteria, p. 319. ASM Press, Washington, DC.
12. Martinez A, Torello S Kolter R (1999) Sliding motility in mycobacteria. J Bacteriol 181 : 7331 7338.
13. Molle V, Kremer L, Girard-Blanc C, Besra GS, Cozzone AJ Prost JF (2003) An FHA phosphoprotein recognition domain mediates protein EmbR phosphorylation by PknH, a Ser/Thr protein kinase from Mycobacterium tuberculosis. Biochemistry 42 : 15300 15309.
14. Molle V, Soulat D, Jault JM, Grangeasse C, Cozzone AJ Prost JF (2004) Two FHA domains on an ABC transporter, Rv1747, mediate its phosphorylation by PknF, a Ser/Thr protein kinase from Mycobacterium tuberculosis. FEMS Microbiol Lett 234 : 215 223.
15. Recht J Kolter R (2001) Glycopeptidolipid acetylation affects sliding motility and biofilm formation in Mycobacterium smegmatis. J Bacteriol 183 : 5718 5724.
16. Recht J, Martinez A, Torello S Kolter R (2000) Genetic analysis of sliding motility in Mycobacterium smegmatis. J Bacteriol 182 : 4348 4351.
17. Sambrook J, Fritsch E Maniatis T (1989) Strategies for cloning in plasmid vectors. Molecular Cloning: A Laboratory Manual (Harbor CS, ed), pp. 1.31 1.38. Cold Spring Harbor Laboratory Press, New York.
18. Snapper SB, Melton RE, Mustafa S, Kieser T Jacobs WR Jr. (1990) Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol Microbiol 4 : 1911 1919.
19. Stover CK, de la Cruz VF, Fuerst TR et al 1991) New use of BCG for recombinant vaccines. Nature 351 : 456 460.
20. Vilcheze C, Morbidoni HR, Weisbrod TR, Iwamoto H, Kuo M, Sacchettini JC Jacobs WR Jr. (2000) Inactivation of the inhA-encoded fatty acid synthase II (FASII) enoyl-acyl carrier protein reductase induces accumulation of the FASI end products and cell lysis of Mycobacterium smegmatis. J Bacteriol 182 : 4059 4067.