A colorimetric assay for studying effector secretion through the bacterial type III secretion system

FEMS Microbiology Letters

Volumn 278, Issue 1, 2008, Pages 36-42

  Miyake, Masami ^a   Sakane, Sadatsugu ^a   Kobayashi, Chiho ^a   Hanajima Ozawa, Miyuki ^a   Fukui, Aya ^a   Kamitani, Shigeki ^a   Horiguchi, Yasuhiko ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Adenylate cyclase toxin; Caco 2; Enteropathogenic; EPEC; Translocated intimin receptor

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENYLATE CYCLASE; BACTERIAL PROTEIN; CYCLIC AMP; DEOXYGLUCOSE; EFFECTOR PROTEIN; ESPF PROTEIN; GENE PRODUCT; HYBRID PROTEIN; MAP PROTEIN; SIGNAL PEPTIDE; SODIUM AZIDE; TIR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BORDETELLA; CELL DEATH; CELL VIABILITY; CELLULAR DISTRIBUTION; COLORIMETRY; CONTROLLED STUDY; EFFECTOR CELL; ENTEROPATHOGENIC ESCHERICHIA COLI; GENE TRANSLOCATION; MICROBIAL ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION;

ADENYLATE CYCLASE; ADENYLATE CYCLASE TOXIN; ADHESINS, BACTERIAL; ANTIMICROBIAL CATIONIC PEPTIDES; CACO-2 CELLS; CARRIER PROTEINS; COLORIMETRY; ENTEROPATHOGENIC ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; PROTEIN TRANSPORT; RECEPTORS, CELL SURFACE; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); BORDETELLA; ESCHERICHIA COLI;

EID: 36749057905     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2007.00943.x     Document Type: Article

References (33)

1. Abe A, de Grado M, Pfuetzner RA et al 1999) Enteropathogenic Escherichia coli translocated intimin receptor, Tir, requires a specific chaperone for stable secretion. Mol Microbiol 33 : 1162 1175.
2. Au DC, Masure HR Storm DR (1989) Site-directed mutagenesis of lysine 58 in a putative ATP-binding domain of the calmodulin-sensitive adenylate cyclase from Bordetella pertussis abolishes catalytic activity. Biochemistry 28 : 2772 2776.
3. Betsou F, Sebo P Guiso N (1993) CyaC-mediated activation is important not only for toxic but also for protective activities of Bordetella pertussis adenylate cyclase-hemolysin. Infect Immun 61 : 3583 3589.
4. Charpentier X Oswald E (2004) Identification of the secretion and translocation domain of the enteropathogenic and enterohemorrhagic Escherichia coli effector Cif, using TEM-1 beta-lactamase as a new fluorescence-based reporter. J Bacteriol 186 : 5486 5495.
5. Crane JK, McNamara BP Donnenberg MS (2001) Role of EspF in host cell death induced by enteropathogenic Escherichia coli. Cell Microbiol 3 : 197 211.
6. Crawford JA Kaper JB (2002) The N-terminus of enteropathogenic Escherichia coli (EPEC) Tir mediates transport across bacterial and eukaryotic cell membranes. Mol Microbiol 46 : 855 868.
7. Dean P, Maresca M, Schüller S, Phillips AD Kenny B (2006) Potent diarrheagenic mechanism mediated by the cooperative action of three enteropathogenic Escherichia coli-injected effector proteins. Proc Natl Acad Sci USA 103 : 1876 1881.
8. Deng W, Li Y, Hardwidge PR et al 2005) Regulation of type III secretion hierarchy of translocators and effectors in attaching and effacing bacterial pathogens. Infect Immun 73 : 2135 2146.
9. Deng W, Puente JL, Gruenheid S et al 2004) Dissecting virulence: systematic and functional analyses of a pathogenicity island. Proc Natl Acad Sci USA 101 : 3597 3602.
10. Gálan JE Wolf-Watz H (2006) Protein delivery into eukaryotic cells by type III secretion machines. Nature 444 : 567 573.
11. Garcia JT, Ferracci F, Jackson MW et al 2006) Measurement of effector protein injection by type III and type IV secretion systems by using a 13-residue phosphorylatable glycogen synthase kinase tag. Infect Immun 74 : 5645 5657.
12. Gauthier A, de Grado M Finlay BB (2000) Mechanical fractionation reveals structural requirements for enteropathogenic Escherichia coli Tir insertion into host membranes. Infect Immun 68 : 4344 4348.
13. Hayward RD, Leong JM, Koronakis V Campellone KG (2006) Exploiting pathogenic Escherichia coli to model transmembrane receptor signalling. Nat Rev Microbiol 4 : 358 370.
14. He SY, Nomura K Whittam TS (2004) Type III protein secretion mechanism in mammalian and plant pathogens. Biochim Biophys Acta 1694 : 181 206.
15. +: the implication of a protective role of alpha B-crystallin accumulation in reactive glia. Brain Res 673 : 47 52.
16. 1 macrophages. Am J Physiol 249 : C256 C266.
17. Kaper JB, Nataro JP Mobley HLT (2004) Pathogenic Escherichia coli. Nat Rev Microbiol 2 : 123 140.
18. Kenny B Jepson M (2000) Targeting of an enteropathogenic Escherichia coli (EPEC) effector protein to host mitochondria. Cell Microbiol 2 : 579 590.
19. Korzeniewski C Callewaert DM (1983) An enzyme-release assay for natural cytotoxicity. J Immunol Methods 64 : 313 320.
20. Ladant D Ullmann A (1999) Bordetella pertussis adenylate cyclase: a toxin with multiple talents. Trends Microbiol 7 : 172 176.
21. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680 685.
22. Levine MM, Bergquist EJ, Nalin DR, Waterman DH, Hornick RB, Young CR Sotman S (1978) Escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet i : 1119 1122.
23. Ma C, Wickham ME, Guttman JA et al 2006) Citrobacter rodentium infection causes both mitochondrial dysfunction and intestinal epithelial barrier disruption in vivo: role of mitochondrial associated protein (Map). Cell Microbiol 8 : 1669 1686.
24. 2/M transition. Mol Microbiol 50 : 1553 1567.
25. McNamara BP, Koutsouris A, O'Connell CB, Nougayréde JP, Donnenberg MS Hecht G (2001) Translocated EspF protein from enteropathogenic Escherichia coli disrupts host intestinal barrier function. J Clin Invest 107 : 621 629.
26. Miao EA Miller SI (2000) A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci USA 97 : 7539 7544.
27. Miyake M, Hanajima M, Matsuzawa T, Kobayashi C, Minami M, Abe A Horiguchi Y (2005) Binding of intimin with Tir on the bacterial surface is prerequisite for the barrier disruption induced by enteropathogenic Escherichia coli. Biochem Biophys Res Commun 337 : 922 927.
28. Moley KH Mueckler MM (2000) Glucose transport and apoptosis. Apoptosis 5 : 99 105.
29. Nagai T, Abe A Sasakawa C (2005) Targeting of enteropathogenic Escherichia coli EspF to host mitochondria is essential for bacterial pathogenesis: critical role of the 16th leucine residue in EspF. J Biol Chem 280 : 2998 3011.
30. Sekiya K, Ohishi M, Ogino T, Tamano K, Sasakawa C Abe A (2001) Supermolecular structure of the enteropathogenic Escherichia coli type III secretion system and its direct interaction with the EspA-sheath-like structure. Proc Natl Acad Sci USA 98 : 11638 11643.
31. Tobe T, Beatson SA, Taniguchi H et al 2006) An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination. Proc Natl Acad Sci USA 103 : 14941 14946.
32. Vallance BA Finlay BB (2000) Exploitation of host cells by enteropathogenic Escherichia coli. Proc Natl Acad Sci USA 97 : 8799 8806.
33. Wolff C, Nisan I, Hanski E, Frankel G Rosenshine I (1998) Protein translocation into host epithelial cells by infecting enteropathogenic Escherichia coli. Mol Microbiol 28 : 143 155.