Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry

Volumn 41, Issue 1-2, 2008, Pages 103-108

  Väänänen, Riina Minna ^a   Rissanen, Maria ^a   Kauko, Otto ^a   Junnila, Siina ^a   Väisänen, Ville ^a   Nurmi, Jussi ^a   Alanen, Kalle ^a   Nurmi, Martti ^a   Pettersson, Kim ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

Circulating cells; Prostate cancer antigen 3; Prostate specific antigen; Reverse transcriptase polymerase chain reaction

Indexed keywords

LANTHANIDE; PROSTATE CANCER ANTIGEN 3; TUMOR ANTIGEN; UNCLASSIFIED DRUG;

ANTIGEN EXPRESSION; ARTICLE; BLOOD SAMPLING; CANCER PATIENT; CONTROLLED STUDY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE DISEASE; REAL TIME POLYMERASE CHAIN REACTION;

AGED; AGED, 80 AND OVER; ANTIGENS, NEOPLASM; BACTERIAL INFECTIONS; FLUORESCENT DYES; HUMANS; MALE; MIDDLE AGED; MODELS, BIOLOGICAL; NEOPLASM METASTASIS; NEOPLASM STAGING; PROSTATE-SPECIFIC ANTIGEN; PROSTATIC HYPERPLASIA; PROSTATIC NEOPLASMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, NEOPLASM; SPECIMEN HANDLING;

EID: 36749035427     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2007.10.009     Document Type: Article

References (20)

1. Bussemakers M.J., van Bokhoven A., Verhaegh G.W., et al. DD3: a new prostate-specific gene, highly overexpressed in prostate cancer. Cancer Res 59 (1999) 5975-5979
2. Gandini O., Luci L., Stigliano A., et al. Is DD3 a new prostate-specific gene?. Anticancer Res 23 (2003) 305-308
3. Verhaegh G.W., de Kok J.B., Hessels D., Smit F., and Schalken J.A. Cancer Res 63 (2003) 4748-4749 [Reply]
4. de Kok J.B., Verhaegh G.W., Roelofs R.W., et al. DD3(PCA3), a very sensitive and specific marker to detect prostate tumors. Cancer Res 62 (2002) 2695-2698
5. Hessels D., Klein Gunnewiek J.M., van Oort I., et al. DD3(PCA3)-based molecular urine analysis for the diagnosis of prostate cancer. Eur Urol 44 (2003) 8-15
6. Tinzl M., Marberger M., Horvath S., and Chypre C. DD3PCA3 RNA analysis in urine-a new perspective for detecting prostate cancer. Eur Urol 46 (2004) 182-186
7. Groskopf J., Aubin S.M., Deras I.L., et al. APTIMA PCA3 molecular urine test: development of a method to aid in the diagnosis of prostate cancer. Clin Chem 52 (2006) 1089-1095
8. Gandini O, Santulli M, Cardillo MR, Stigliano A, Toscano V. Correspondence re: J. B. de Kok et al. DD3, A very sensitive and specific marker to detect prostate tumors. Cancer Res., 62:2695-2698, 2002 [Letter to the Editor]. Cancer Res. 2003;63:4747.
9. Jung M, Xu C, Spethmann J, et al. Re: Hessels D, Klein Gunnewiek JMT, van Oort I, Karthaus HFM, van Leenders GJL, van Balken B, Kiemeney LA, Witjes JA, Schalken JA. DD3(PCA3)-based molecular urine analysis for the diagnosis of prostate cancer. Eur Urol 2003;44:8-16 [Letter to the Editor]. Eur Urol. 2004;46:271-2.
10. Marangoni K., Neves A.F., Cardoso A.M., Santos W.K., Faria P.C., and Goulart L.R. The endothelial nitric oxide synthase Glu-298-Asp polymorphism and its mRNA expression in the peripheral blood of patients with prostate cancer and benign prostatic hyperplasia. Cancer Detect Prev 30 (2006) 7-13
11. Dheda K., Huggett J.F., Bustin S.A., Johnson M.A., Rook G., and Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 37 (2004) 112-114 [116, 118-9]
12. Huggett J., Dheda K., Bustin S., and Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6 (2005) 279-284
13. Nurmi J., Wikman T., Karp M., and Lövgren T. High-performance real-time quantitative RT-PCR using lanthanide probes and a dual-temperature hybridization assay. Anal Chem 74 (2002) 3525-3532
14. Nurmi J., Lilja H., and Ylikoski A. Time-resolved fluorometry in end-point and real-time PCR quantification of nucleic acids. Luminescence 15 (2000) 381-388
15. Lundwall Å., and Lilja H. Molecular cloning of human prostate-specific antigen cDNA. FEBS Lett 214 (1987) 317-322
16. Ylikoski A., Sjöroos M., Lundwall Å., et al. Quantitative reverse transcription-PCR assay with an internal standard for the detection of prostate-specific antigen mRNA. Clin Chem 45 (1999) 1397-1407
17. Ylikoski A., Karp M., Pettersson K., Lilja H., and Lövgren T. Simultaneous quantification of human glandular kallikrein 2 and prostate-specific antigen mRNAs in peripheral blood from prostate cancer patients. J. Mol. Diagn. 3 (2001) 111-122
18. Rissanen M., Helo P., Väänänen R.-M., et al. Novel homogeneous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood. Clin Biochem 40 (2007) 111-118
19. Nurmi J., Ylikoski A., Soukka T., Karp M., and Lövgren T. A new label technology for the detection of specific polymerase chain reaction products in a closed tube. Nucleic Acids Res 28 (2000) E28
20. Schamhart D.H., Maiazza R., and Kurth K.H. Identification of circulating prostate cancer cells: a challenge to the clinical implementation of molecular biology (review). Int J Oncol 26 (2005) 565-577