Legionella in clinical specimens and hospital water supply facilities: Molecular detection and genotyping of the isolates

Medical Principles and Practice

Volumn 17, Issue 1, 2008, Pages 49-55

  Qasem, J A ^a   Mustafa, A S ^b   Khan, Z U ^b  

^a MANUFACTURING ENGINEERING DEPARTMENT   (Kuwait)

^b KUWAIT UNIVERSITY   (Kuwait)

Author keywords

Legionella; Polymerase chain reaction; Random amplification of polymorphic DNA; Typing methods

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; BODY FLUID; CONTROLLED STUDY; GENOTYPE; HOSPITAL; HUMAN; LEGIONELLA; LEGIONELLA PNEUMOPHILA; LUNG LAVAGE; MOLECULAR TYPING; NONHUMAN; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; SEROLOGY; SPUTUM; THROAT CULTURE; VIRUS PNEUMONIA; WATER ANALYSIS; WATER CONTAMINATION; WATER SUPPLY;

CULTURE TECHNIQUES; EQUIPMENT AND SUPPLIES, HOSPITAL; GENOTYPE; HUMANS; LEGIONELLA; LEGIONELLOSIS; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; WATER MICROBIOLOGY; WATER SUPPLY;

EID: 36749001666     PISSN: 10117571     EISSN: None     Source Type: Journal    
DOI: 10.1159/000109590     Document Type: Article

References (29)

1. Costa J, Tiago I, da Costa MS, Verissimo A: Presence and persistence of Legionella spp in groundwater. Appl Environ Microbiol 2005;71:663-671.
2. Perola O, Kauppinen J, Kusnetsov J, Karkkainen UM, Luck PC, Katila ML: Persistent Legionella pneumophila colonization of a hospital water supply: efficacy of control methods and a molecular epidemiological analysis. APMIS 2005;113:45-53.
3. Stout JE, Yu VL: Hospital-acquired Legionnaires' disease: new developments. Curr Opin Infect Dis 2003;16:337-341.
4. O'Neill E, Humphreys H: Surveillance of hospital water and primary prevention of nosocomial legionellosis: what is the evidence? J Hosp Infect 2005;59:273-279.
5. Hindiyeh M, Carroll KC: Laboratory diagnosis of atypical pneumonia. Semin Respir Infect 2000;15:101-113.
6. Waterer GW, Baselski VS, Wunderink RG: Legionella and community-acquired pneumonia: a review of current diagnostic tests from a clinician's viewpoint. Am J Med 2001;110:41-48.
7. Den Boer JW, Yzerman EP: Diagnosis of Legionella infection in Legionnaires' disease. Eur J Clin Microbiol Infect Dis 2004;23:871-878.
8. Mahbubani MH, Bej AK, Miller R, Haff L, DiCesare J, Atlas RM: Detection of Legionella with polymerase chain reaction and gene probe methods. Mol Cell Probes 1990;4:175-187.
9. Murdoch DR, Walford EJ, Jennings LC Light GJ, Schousboe MI, Chereshsky AY, Chambers ST, Town GI: Use of the polymerase chain reaction to detect Legionella DNA in urine and serum samples from patients with pneumonia. Clin Infect Dis 1996;23:475-480.
10. Matsiota-Bernard P, Waser S, Vrioni G: Detection of Legionella pneumophila DNA in urine and serum samples from patients with pneumonia. Clin Microbiol Infect 2000;6:223-225.
11. Joly JR, Winn WC: Correlation of subtypes of Legionella pneumophila defined by monoclonal antibodies with epidemiological classification of cases and environmental sources. J Infect Dis 1984;150:667-671.
12. McKinney RM, Kuffner TA, Bibb WF, Nokkaew C, Wells DE, Arnow PM, Woods TC, Plikaytis BD: Antigenic and genetic variation in Legionella pneumophila serogroup 6. J Clin Microbiol 1989;27:738-742.
13. Edelstein PH: Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol 1981;14:298-303.
14. Mustafa AS, Ahmed A, Abal AT, Chugh TD: Establishment and evaluation of a multiplex polymerase chain reaction for detection of mycobacteria and specific identification of Mycobacterium tuberculosis complex. Tuber Lung Dis 1995;76:336-343.
15. Qasem JA, Khan ZU, Mustafa AS: Diagnosis of nocardiosis by polymerase chain reaction: an experimental study in mice. Microbiol Res 2001;156:317-322.
16. Telenti A, Marchesi F, Balz M, Bally F, Bottger EC, Bodmer T: Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993;31:175-178.
17. Amoudy HA, Al-Turab MB, Mustafa AS: Identification of transcriptionally active open reading frames within the RD1 genomic segment of Mycobacterium tuberculosis. Med Princ Pract 2006;15:137-144.
18. Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990;18:6531-6535.
19. Welsh J, McClelland M: Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990;18:7213-7218.
20. Kumar S, Tamura K, Nei M: MEGA: Molecular Evolutionary Genetics Analysis, Version 1.02. University Park, Pennsylvania State University, 1993.
21. Vaneechoutte M, Van Eldere J: The possibilities and limitations of nucleic acid amplification technology in diagnostic microbiology. J Med Microbiol 1997;46:188-194.
22. Donlan RM: Biofilm formation: a clinically relevant microbiological process. Clin Infect Dis 2001;33:1387-1392.
23. Fliermans CB: Ecology of Legionella: from data to knowledge with a little wisdom. Microb Ecol 1996;32:203-228.
24. Verissimo A, Marrao G, da Silva FG, da Costa MS: Distribution of Legionella spp in hydrothermal areas in continental Portugal and the island of Sao Miguel, Azores. Appl Environ Microbiol 1991;57:2921-2927.
25. Luck PC, Leupold I, Hlawitschka M, et al: Prevalence of Legionella species, serogroups and monoclonal subgroups in hot water systems in south-eastern Germany. Zentralbl Hyg Umweltmed 1993;193:450-460.
26. Zeitz B, Wiese F, Brengelmann F, Dunkelberg H: Presence of Legionellaceae in warm water supplies and typing of stains by polymerase chain reaction. Epidemiol Infect 2001;126:147-152.
27. Ta AC, Stout JE, Yu Vl, Wagener MM: Comparison of culture methods for monitoring Legionella species in hospital potable water systems and recommendation for standardization of such methods. J Clin Microbiol 1995;33:2118-2123.
28. Ieven M, Goossens H: Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory. Clin Microbiol Rev 1997;10:242-256.
29. Ruef C: Nosocomial Legionnaires' disease - Strategies for prevention. J Microbiol Methods 1998;33:81-91.