The zinc finger domain of Wilms' tumor 1 suppressor gene (WT1) behaves as a dominant negative, leading to abrogation of WT1 oncogenic potential in breast cancer cells

Breast Cancer Research

Volumn 9, Issue 4, 2007, Pages

  Han, Youqi ^a   San Marina, Serban ^a   Yang, Lin ^a   Khoury, Haytham ^a   Minden, Mark D ^a  

^a PRINCESS MARGARET HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHIREGULIN; LUCIFERASE; LYSINE; MYC PROTEIN; PROTEIN BCL 2; SERINE; TELOMERASE REVERSE TRANSCRIPTASE; THREONINE; WT1 PROTEIN; ZINC FINGER PROTEIN; GLYCOPROTEIN; MYC PROTEIN, HUMAN; SIGNAL PEPTIDE; TELOMERASE; TERT PROTEIN, HUMAN; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CANCER GENE THERAPY; CANCER INHIBITION; CELL PROLIFERATION; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; DRUG MECHANISM; EXON; GENE EXPRESSION; GENE INSERTION SEQUENCE; GENE TARGETING; HUMAN; HUMAN CELL; NORTHERN BLOTTING; ONCOGENE C MYC; PROMOTER REGION; PROTEIN DOMAIN; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; TUMOR SUPPRESSOR GENE; WESTERN BLOTTING; BREAST TUMOR; CELL CULTURE; CELL TRANSFORMATION; CLONOGENIC ASSAY; DOMINANT GENE; GEL MOBILITY SHIFT ASSAY; GENETICS; METABOLISM; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE;

APOPTOSIS; BLOTTING, NORTHERN; BLOTTING, WESTERN; BREAST NEOPLASMS; CELL PROLIFERATION; CELL TRANSFORMATION, NEOPLASTIC; CHROMATIN IMMUNOPRECIPITATION; COLONY-FORMING UNITS ASSAY; ELECTROPHORETIC MOBILITY SHIFT ASSAY; GENES, DOMINANT; GLYCOPROTEINS; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LUCIFERASES; PROTEIN STRUCTURE, TERTIARY; PROTO-ONCOGENE PROTEINS C-MYC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TELOMERASE; TUMOR CELLS, CULTURED; WT1 PROTEINS; ZINC FINGERS;

EID: 36748998856     PISSN: 14655411     EISSN: 1465542X     Source Type: Journal    
DOI: 10.1186/bcr1743     Document Type: Article

References (39)

1. Buckler AJ Pelletier J Haber DA Glaser T Housman DE Isolation, characterization, and expression of the murine Wilms' tumor gene (WT1) during kidney development Mol Cell Biol 1991, 11:1707-1712. 369476 1671709
2. Haber DA Buckler AJ Glaser T Call KM Pelletier J Sohn RL Douglass EC Housman DE An internal deletion within an 11p13 zinc finger gene contributes to the development of Wilms' tumor Cell 1990, 61:1257-1269. 10.1016/0092-8674(90)90690-G 2163761
3. Haber DA Park S Maheswaran S Englert C Re GG Hazen-Martin DJ Sens DA Garvin AJ WT1-mediated growth suppression of Wilms tumor cells expressing a WT1 splicing variant Science 1993, 262:2057-2059. 10.1126/ science.8266105 8266105
4. Park S Tomlinson G Nisen P Haber DA Altered trans-activational properties of a mutated WT1 gene product in a WAGR-associated Wilms' tumor Cancer Res 1993, 53:4757-4760. 8402654
5. Bruening W Gros P Sato T Stanimir J Nakamura Y Housman D Pelletier J Analysis of the 11p13 Wilms' tumor suppressor gene (WT1) in ovarian tumors Cancer Invest 1993, 11:393-399. 8324644
6. Silberstein GB Van Horn K Strickland P Roberts CT Jr Daniel CW Altered expression of the WT1 wilms tumor suppressor gene in human breast cancer Proc Natl Acad Sci USA 1997, 94:8132-8137. 21569 9223327 10.1073/ pnas.94.15.8132
7. Oji Y Yano M Nakano Y Abeno S Nakatsuka S Ikeba A Yasuda T Fujiwara Y Takiguchi S Yamamoto H Overexpression of the Wilms' tumor gene WT1 in esophageal cancer Anticancer Res 2004, 24:3103-3108. 15510596
8. Oji Y Nakamori S Fujikawa M Nakatsuka S Yokota A Tatsumi N Abeno S Ikeba A Takashima S Tsujie M Overexpression of the Wilms' tumor gene WT1 in pancreatic ductal adenocarcinoma Cancer Sci 2004, 95:583-587. 10.1111/ j.1349-7006.2004.tb02490.x 15245594
9. Keilholz U Menssen HD Gaiger A Menke A Oji Y Oka Y Scheibenbogen C Stauss H Thiel E Sugiyama H Wilms' tumour gene 1 (WT1) in human neoplasia Leukemia 2005, 19:1318-1323. 10.1038/sj.leu.2403817 15920488
10. Miyoshi Y Ando A Egawa C Taguchi T Tamaki Y Tamaki H Sugiyama H Noguchi S High expression of Wilms' tumor suppressor gene predicts poor prognosis in breast cancer patients Clin Cancer Res 2002, 8:1167-1171. 12006533
11. Karakas T Miething CC Maurer U Weidmann E Ackermann H Hoelzer D Bergmann L The coexpression of the apoptosis-related genes bcl-2 and wt1 in predicting survival in adult acute myeloid leukemia Leukemia 2002, 16:846-854. 10.1038/sj.leu.2402434 11986946
12. Inoue K Sugiyama H Ogawa H Nakagawa M Yamagami T Miwa H Kita K Hiraoka A Masaoka T Nasu K WT1 as a new prognostic factor and a new marker for the detection of minimal residual disease in acute leukemia Blood 1994, 84:3071-3079. 7949179
13. Scharnhorst V van der Eb AJ Jochemsen AG WT1 proteins: Functions in growth and differentiation Gene 2001, 273:141-161. 10.1016/ S0378-1119(01)00593-5 11595161
14. Madden SL Cook DM Rauscher FJ III A structure-function analysis of transcriptional repression mediated by the WT1, Wilms' tumor suppressor protein Oncogene 1993, 8:1713-1720. 8510918
15. Haber DA Sohn RL Buckler AJ Pelletier J Call KM Housman DE Alternative splicing and genomic structure of the Wilms tumor gene WT1 Proc Natl Acad Sci USA 1991, 88:9618-9622. 52769 1658787 10.1073/pnas.88.21.9618
16. Nakagama H Heinrich G Pelletier J Housman DE Sequence and structural requirements for high-affinity DNA binding by the WT1 gene product Mol Cell Biol 1995, 15:1489-1498. 230373 7862142
17. Lee SB Haber DA Wilms tumor and the WT1 gene Exp Cell Res 2001, 264:74-99. 10.1006/excr.2000.5131 11237525
18. Wang ZY Qiu QQ Enger KT Deuel TF A second transcriptionally active DNA-binding site for the Wilms tumor gene product, WT1 Proc Natl Acad Sci USA 1993, 90:8896-8900. 47467 8415628 10.1073/pnas.90.19.8896
19. Dehbi M Ghahremani M Lechner M Dressler G Pelletier J The paired-box transcription factor, PAX2, positively modulates expression of the Wilms' tumor suppressor gene (WT1) Oncogene 1996, 13:447-453. 8760285
20. McConnell MJ Cunliffe HE Chua LJ Ward TA Eccles MR Differential regulation of the human Wilms tumour suppressor gene (WT1) promoter by two isoforms of PAX2 Oncogene 1997, 14:2689-2700. 10.1038/sj.onc.1201114 9178767
21. Dehbi M Pelletier J PAX8-mediated activation of the wt1 tumor suppressor gene EMBO J 1996, 15:4297-4306. 452155 8861958
22. Siehl JM Thiel E Heufelder K Snarski E Schwartz S Mailander V Keilholz U Possible regulation of Wilms' tumour gene 1 (WT1) expression by the paired box genes PAX2 and PAX8 and by the haematopoietic transcription factor GATA-1 in human acute myeloid leukaemias Br J Haematol 2003, 123:235-242. 10.1046/j.1365-2141.2003.04622.x 14531904
23. Scharnhorst V Menke AL Attema J Haneveld JK Riteco N Van Steenbrugge GJ van der Eb AJ Jochemsen AG EGR-1 enhances tumor growth and modulates the effect of the Wilms' tumor 1 gene products on tumorigenicity Oncogene 2000, 19:791-800. 10.1038/sj.onc.1203390 10698497
24. Cohen HT Bossone SA Zhu G McDonald GA Sukhatme VP Sp1 is a critical regulator of the Wilms' tumor-1 gene J Biol Chem 1997, 272:2901-2913. 10.1074/jbc.272.11.7151 9006935
25. Rupprecht HD Drummond IA Madden SL Rauscher FJ III Sukhatme VP The Wilms' tumor suppressor gene WT1 is negatively autoregulated J Biol Chem 1994, 269:6198-6206. 8119964
26. Hewitt SM Hamada S McDonnell TJ Rauscher FJ III Saunders GF Regulation of the proto-oncogenes bcl-2 and c-myc by the Wilms' tumor suppressor gene WT1 Cancer Res 1995, 55:5386-5389. 7585606
27. Han Y San Marina S Liu J Minden MD Transcriptional activation of c-myc proto-oncogene by WT1 protein Oncogene 2004, 23:6933-6941. 10.1038/ sj.onc.1207609 15286719
28. Hewitt SM Fraizer GC Wu YJ Rauscher FJ III Saunders GF Differential function of Wilms' tumor gene WT1 splice isoforms in transcriptional regulation J Biol Chem 1996, 271:8588-8592. 10.1074/jbc.271.15.8588 8621487
29. Fraizer GC Wu YJ Hewitt SM Maity T Ton CC Huff V Saunders GF Transcriptional regulation of the human Wilms' tumor gene (WT1). Cell type-specific enhancer and promiscuous promoter J Biol Chem 1994, 269:8892-8900. 8132626
30. Hofmann W Royer HD Drechsler M Schneider S Royer-Pokora B Characterization of the transcriptional regulatory region of the human WT1 gene Oncogene 1993, 8:3123-3132. 8414514
31. Reddy JC Morris JC Wang J English MA Haber DA Shi Y Licht JD WT1-mediated transcriptional activation is inhibited by dominant negative mutant proteins J Biol Chem 1995, 270:10878-10884. 10.1074/ jbc.270.41.24502 7738027
32. Moffett P Bruening W Nakagama H Bardeesy N Housman D Housman DE Pelletier J Antagonism of WT1 activity by protein self-association Proc Natl Acad Sci USA 1995, 92:11105-11109. 40580 7479946 10.1073/ pnas.92.24.11105
33. Holmes G Boterashvili S English M Wainwright B Licht J Little M Two N-terminal self-association domains are required for the dominant negative transcriptional activity of WT1 Denys-Drash mutant proteins Biochem Biophys Res Commun 1997, 233:723-728. 10.1006/bbrc.1997.6545 9168922
34. Oji Y Ogawa H Tamaki H Oka Y Tsuboi A Kim EH Soma T Tatekawa T Kawakami M Asada M Expression of the Wilms' tumor gene WT1 in solid tumors and its involvement in tumor cell growth Jpn J Cancer Res 1999, 90:194-204. 10189890
35. Phelan SA Lindberg C Call KM Wilms' tumor gene, WT1, mRNA is down-regulated during induction of erythroid and megakaryocytic differentiation of K562 cells Cell Growth Differ 1994, 5:677-686. 8086342
36. Malik KT Poirier V Ivins SM Brown KW Autoregulation of the human WT1 gene promoter FEBS Lett 1994, 349:75-78. 10.1016/0014-5793(94)00642-3 8045305
37. Bickmore WA Oghene K Little MH Seawright A van HV Hastie ND Modulation of DNA binding specificity by alternative splicing of the Wilms tumor wt1 gene transcript Science 1992, 257:235-237. 10.1126/science.1321494 1321494
38. Drummond IA Rupprecht HD Rohwer-Nutter P Lopez-Guisa JM Madden SL Rauscher FJ III Sukhatme VP DNA recognition by splicing variants of the Wilms' tumor suppressor, WT1 Mol Cell Biol 1994, 14:3800-3809. 358747 8196623
39. Laity JH Dyson HJ Wright PE Molecular basis for modulation of biological function by alternate splicing of the Wilms' tumor suppressor protein Proc Natl Acad Sci USA 2000, 97:11932-11935. 17272 11050227 10.1073/ pnas.97.22.11932