Impact of zooplankton grazing on the excystation, viability, and infectivity of the protozoan pathogens Cryptosporidium parvum and Giardia lamblia

Applied and Environmental Microbiology

Volumn 73, Issue 22, 2007, Pages 7277-7282

  Connelly, S J ^a   Wolyniak, E A ^b   Dieter, K L ^a,c   Williamson, C E ^a   Jellison, K L ^b  

^a MIAMI UNIVERSITY   (United States)

^b Lehigh University   (United States)

^c 212 Pearson Hall ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGAE; CELL CULTURE; CELL GROWTH; PATHOGENS;

BIOLOGICAL FILTERS; CRYPTOSPORIDIUM PARVUM OOCYSTS; FRESHWATER; GRAZING PRESSURES; MECHANICAL DISRUPTION; ZOOPLANKTON GRAZERS;

PROTOZOA;

SURFACE WATER;

CRUSTACEAN; CYST; ENVIRONMENTAL IMPACT; FRESHWATER ECOSYSTEM; GRAZING; PROTOZOAN; ZOOPLANKTON;

ARTICLE; BIOFILTER; CELL CULTURE; CRYPTOSPORIDIUM PARVUM; DAPHNIA; DAPHNIA PULICARIA; GIARDIA LAMBLIA; GRAZING; GREEN ALGA; LIGHT DARK CYCLE; LOW TEMPERATURE; NONHUMAN; OOCYST; PARASITOSIS; ROTATION; SELENASTRUM CAPRICORNUTUM; ZOOPLANKTON;

ANIMALS; CELL LINE, TUMOR; CRYPTOSPORIDIUM PARVUM; DAPHNIA; ECOSYSTEM; FRESH WATER; GIARDIA LAMBLIA; HUMANS; MICROSCOPY, FLUORESCENCE; OOCYSTS; SPORES, PROTOZOAN; ZOOPLANKTON;

ALGAE; CRYPTOSPORIDIUM PARVUM; DAPHNIA PULICARIA; GIARDIA INTESTINALIS; PROTOZOA; SELENASTRUM CAPRICORNUTUM;

EID: 36649030163     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01206-07     Document Type: Article

References (22)

1. Agasild, H., and T. Nõges. 2005. Cladoceran and rotifer grazing on bacteria and phytoplankton in two shallow eutrophic lakes: in situ measurement with fluorescent microspheres. J. Plank. Res. 27:1155-1174.
2. Armengol, X., L. Boronat, A. Camacho, and W. A. Wurtsbaugh. 2001. Grazing by a dominant rotifer Conochilus unicornis Rousselet in a mountain lake: in situ measurements with synthetic microspheres. Hydrobiologia 446/447: 107-114.
3. Brookes, J. D., J. Antenucci, M. Hipsey, M. D. Burch, N. J. Ashbolt, and C. Ferguson. 2004. Fate and transport of pathogens in lakes and reservoirs. Environ. Int. 30:741-759.
4. Campbell, A. T., L. J. Robertson, and H. V. Smith. 2002. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Appl. Environ. Microbiol. 58:3488-3493.
5. Centers for Disease Control and Prevention. 7 December 2005, posting date. DPDx: laboratory identification of parasites of public health concern. Parasites and health: cryptosporidiosis. http://www.dpd.cdc.gov/dpdx/.
6. Cyr, H., and M. L. Pace. 1992. Grazing by zooplankton and its relationship to community structure. Can. J. Fish Aquat. Sci. 49:1455-1465.
7. Gardner, T. B., and D. R. Hill. 2001. Treatment of giardiasis. Clin. Microbiol. Rev. 14:114-128.
8. Hsu, B. M., and H. H. Yeh. 2003. Removal of Giardia and Cryptosporidium in drinking water treatment: a pilot-scale study. Water Res. 37:1111-1117.
9. Pokorny, N. J., S. C. Weir, R. A. Carreno, J. T. Trevors, and H. Lee. 2002. Influence of temperature on Cryptosporidium parvum oocyst infectivity in river water samples as detected by tissue culture assay. J. Parasitol. 88:641-643.
10. Rice, E. W., and F. W. Schaefer III. 1981. Improved in vitro excystation procedure for Giardia lamblia cysts. J. Clin. Microbiol. 14:709-710.
11. Robertson, L. J., A. T. Campbell, and H. V. Smith. 1993. In vitro excystation of Cryptosporidium parvum. Parasitology 106:13-19.
12. Rose, J. 1988. Occurrence and significance of Cryptosporidium in water. J. Am. Water Works Assoc. 2:53-58.
13. Slifko, T. R., D. Friedman, J. B. Rose, and W. Jakubowski. 1997. An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture. Appl. Environ. Microbiol. 63:3669-3675.
14. Slifko, T. R., D. E. Huffman, and J. B. Rose. 1999. A most-probable number assay for enumeration of infectious Cryptosporidium parvum oocysts. Appl. Environ. Microbiol. 65:3936-3941.
15. Smith, H. V., and J. B. Rose. 1990. Waterborne cryptosporidiosis. Parasitol. Today 6:8-12.
16. Stott, R., E. May, E. Ramirez, and A. Warren. 2003. Predation of Cryptosporidium oocysts by protozoa and rotifers: implications for water quality and public health. Water Sci. Technol. 47:77-83.
17. Thiriat, L., F. Sidaner, and J. Schwartzbrod. 1998. Determination of Giardia cyst viability in environmental and faecal samples by immunofluorescence, fluorogenic dye staining and differential interference contrast microscopy. Lett. Appl. Microbiol. 26:237-242.
18. Trout, J. M., E. J. Walsh, and R. Fayer. 2002. Rotifers ingest Giardia cysts. J. Parasitol. 88:1038-1040.
19. U.S. Environmental Protection Agency. 1998. Giardia: human health criteria document. Office of Water, U.S. Environmental Protection Agency, Washington, DC.
20. U.S. Environmental Protection Agency. 2001. Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. EPA 821-R-01-026. Office of Water, U.S. Environmental Protection Agency, Washington, DC.
21. U.S. Environmental Protection Agency. 2002. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms, 4th ed. U.S. Environmental Protection Agency, Washington, DC.
22. Williamson, C. E., and J. W. Reid. 2001. Copepoda, p. 915-954. In J. H. Thorp and A. P. Covich (ed.), Ecology and classification of North American freshwater invertebrates, 2nd ed. Academic Press, New York, NY.