Establishment of multiplexed, microsphere-based flow cytometric assay for multiple human tumor markers

Acta Pharmacologica Sinica

Volumn 28, Issue 12, 2007, Pages 2011-2018

  Sun, Kai ^a   Wang, Qian ^a   Huang, Xiao Hui ^a   Zhen, Mao Chuan ^a   Li, Wen ^a   Zhang, Long Juan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Fluorescent microsphere; Microsphere based flow cytometric assay; Tumor marker

Indexed keywords

MICROSPHERE; TUMOR MARKER;

ARTICLE; ASSAY; BLOOD SAMPLING; CANCER DIAGNOSIS; CANCER SCREENING; CLINICAL ARTICLE; COMPARATIVE STUDY; COMPUTER PROGRAM; CONTROLLED STUDY; COVALENT BOND; CROSS REACTION; DILUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; HUMAN TISSUE; MEASUREMENT; MICROSPHERE BASED FLOW CYTOMETRIC ASSAY; QUALITY CONTROL; REPRODUCIBILITY; VALIDATION PROCESS;

ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMANS; MICROSPHERES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TUMOR MARKERS, BIOLOGICAL;

EID: 36649009349     PISSN: 16714083     EISSN: 17457254     Source Type: Journal    
DOI: 10.1111/j.1745-7254.2007.00673.x     Document Type: Article

References (18)

1. Sturgeon C. Practice guidelines for tumor marker use in the clinic. Clin Chem 2002 48:1151 9.
2. Cillo U, Navaglia F, Vitale A, Molari A, Basso D, Bassanello M, et al. Clinical significance of alpha-fetoprotein mRNA in blood of patients with hepatocellular carcinoma. Clin Chim Acta 2004 374:129 38.
3. Hulse RE, Kunkler PE, Fedynyshyn JP, Kraig RP. Optimization of multiplexed bead-based cytokine immunoassays for rat serum and brain tissue. J Neurosci Method 2004 136:87 98.
4. Martins TB, Pasi BM, Litwin CM, Hill HR. Heterophile antibody interference in a multiplexed fluorescent microsphere immunoassay for quantitation of cytokines in human serum. Clin Diagn Lab Immunol 2004 11:325 9.
5. Jager W, Velthuis H, Prakken BJ, Kuis W, Rijkers GT. Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells. Clin Diagn Lab Immunol 2003 10:133 9.
6. Tarnok A, Hambsch J, Chen R, Varro R. Cytometric bead array to measure six cytokines in twenty-five microliters of serum. Clin Chem 2003 49:1000 2.
7. Jia XC, Raya R, Zhang L, Foord O, Walker WL, Gallo ML, et al. A novel method of multiplexed competitive antibody binning for the characterization of monoclonal antibodies. J Immunol Methods 2004 288:91 8.
8. Biagini RE, Sammons DL, Smith JP, MacKenzie BA, Striley CA, Semenova V, et al. Comparison of a multiplexed fluorescent covalent microsphere immunoassay and an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins. Clin Diagn Lab Immunol 2004 11:50 5.
9. Khan SS, Smith MS, Reda D, Suffredini AF, McCoy JP. Multiplex bead array assays for detection of soluble cytokines: Comparisons of sensitivity and quantitative values among kits from multiple manufacturers. Cytometry 2004 61:35 9.
10. Jani IV, Janossy G, Brown DW, Mandy F. Multiplexed immunoassays by flow cytometry for diagnosis and surveillance of infectious diseases in resource-poor settings. Lancet Infect Dis 2002 2:243 50.
11. Faucher S, Martel A, Sherring A, Ding T, Malloch L, Kim JE, et al. Protein bead array for the detection of HIV-1 antibodies from fresh plasma and dried-blood-spot specimens. Clin Chem 2004 50:1250 3.
12. Bellisario R, Colinas RJ, Pass KA. Simultaneous measurement of thyroxine and thyrotropin from newborn dried blood-spot specimens using a multiplexed fluorescent microsphere immunoassay. Clin Chem 2000 46:1422 4.
13. Lukacs Z, Mordac C, Kohlschutter A, Kruithof R. Use of microsphere immunoassay for simplified multianalyte screening of thyrotropin and thyroxine in dried blood spots from newborns. Clin Chem 2003 49:335 6.
14. Kellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. Exp Hematol 2002 30:1227 37.
15. Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods 1999 227:41 52.
16. Wong SJ, Demarest VL, Boyle RH, Wang T, Ledizet M, Kar K, et al. Detection of human anti-flavivirus antibodies with a West Nile Virus recombinant antigen microsphere immunoassay. J Clin Microbiol 2004 42:65 72.
17. Kellar KL, Douglass JP. Multiplexed microsphere-based flow cytometric immunoassays for human cytokines. J Immunol Methods 2003 279:277 85.
18. Pataki J, Szabo M, Lantos E, Szekvolgyi L, Molnar M, Hegedus E, et al. Biological microbeads for flow-cytometric immunoassays, enzyme titrations, and quantitative PCR. Cytometry A 2005, 68:45 52.