In vitro and in vivo methods to study protein import into plant mitochondria

Methods in Molecular Biology

Volumn 390, Issue , 2007, Pages 131-150

  Bhushan, Shashi ^a   Pavlov, Pavel F ^a   Rudhe, Charlotta ^a   Glaser, Elzbieta ^a  

^a NONE

Author keywords

Agrobacterium; Chloroplasts; GFP fusion; Mitochondria; Precursor; Presequence; Protein import; Protoplasts; Targeting peptide; Transient expression

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN;

ARTICLE; BIOLOGICAL MODEL; CHLOROPLAST; ELECTROPORATION; IN VITRO STUDY; METABOLISM; METHODOLOGY; MITOCHONDRION; MOLECULAR BIOLOGY; PLANT; POTATO; PROTEIN TRANSPORT; SPINACH;

CHLOROPLASTS; ELECTROPORATION; GREEN FLUORESCENT PROTEINS; MITOCHONDRIA; MODELS, BIOLOGICAL; MOLECULAR BIOLOGY; PLANTS; PROTEIN TRANSPORT; PROTEINS; RECOMBINANT FUSION PROTEINS; SOLANUM TUBEROSUM; SPINACIA OLERACEA;

AGROBACTERIUM; NICOTIANA TABACUM; RHIZOBIUM;

EID: 36549059174     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-466-4:131     Document Type: Article

References (31)

1. Truscott, K. N., Brandner, K., and Pfanner, N. (2003) Mechanisms of protein import into mitochondria. Curr. Biol. 13, R326-337.
2. Gray, M. W., Burger, G., and Lang, B. F. (1999) Mitochondrial evolution. Scienc, 283, 1476-1481.
3. Zhang, X. P. and Glaser, E. (2002) Interaction of plant mitochondrial and chloroplast signal peptides with Hsp70 molecular chaperone. Trends Plant Sci. 1, 14-21.
4. Glaser, E. and Soll, J. (2004) Targeting signals and import machinery of plastids and plant mitochondria. In Molecular Biology and Biotechnology of Plant Organelles: Chloroplasts and Mitochondria (Danieli, H. and Chase, C., eds.) Springer, Dordrecht, The Netherlands, pp. 385-418.
5. Marc, P., Margeot, A., Devaux, F., Blugeon, C., Corral-Debrinski, M., and Jacq, C. (2002). Genome-wide analysis of mRNAs targeted to yeast mitochondria. EMBO Rep. 3, 159-164.
6. Glaser, E. and Dessi, P. (1999) Integration of the mitochondrial processing peptidase into the bel complex of the respiratory chain in plants. J. Bioenerg. Biomembr. 31, 259-274.
7. Stahl, A. Moberg, P., Ytterberg, J., et al. (2002) Isolation and identification of a novel mitochondrial metalloprotease (PreP) that degrades targeting presequences. J. Biol. Chem. 277, 41931-4939.
8. Moberg, P., Ståhl, A., Bhushan, S., et al. (2003) Characterization of a novel zinc metalloprotease involved in degrading signal peptides in mitochondria and chloroplasts. Plant J. 36, 616-628.
9. Bushan, S., Lefebvre, B., Ståhl, A., Boutry, M., and Glaser, E. (2003) Dual targeting and function of a protease in mitochondria and chloroplasts. EMBO Rep. 4, 1073-1078.
10. Ståhl, A., Nilsson, S., Lundberg, P., Bhushan, S., et al. (2005) Two novel targeting peptide degrading proteases, PrePs, in mitochondria and chloroplasts, so similar and still different. J. Mol. Biol. 349, 847-860.
11. 3 chamber for proteolysis. EMBO J. 25, 1977-1986.
12. Creissen, G., Reynolds, H., Xue, Y., and Mullineaux, P. (1995) Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mitochondria in transgenic tobacco. Plant J. 8, 167-175.
13. Small, I., Wintz, H., Akashi, K., and Mireau, H. (1998) Two birds with one stone: genes that encode products targeted to two or more compartments. Plant Mol. Biol. 38, 265-277.
14. Hedtke, B., Borner, T., and Weihe, A. (2000) One RNA polymerase serving two genomes. EMBO Rep. 1, 435-440.
15. Rudhe, C., Chew, O., Whelan, J., and Glaser, E. (2002) A novel in vitro system for simultaneous import of precursor proteins into chloroplast and mitochondria. Plant J. 30, 213-220.
16. Boutry, M., Nagy, F., Poulsen, C., Aoyagi, K., and Chua, N.H. (1987) Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants. Nature 328, 340-342.
17. Schmitz, U. K. and Lonsdale, D. M. (1989) A yeast mitochondrial presequence functions as a signal for targeting to plant mitochondria in-vivo. Plant Cell 1, 783-791.
18. Silva Filho, M. d. C., Wieers, M.-C., Flugge, U.-L, Chaumont, F., and Boutry, M. (1997) Different in vitro and in vivo targeting properties of the transit peptide of a chloroplast envelope inner membrane protein. J. Biol. Chem. 272, 15264-15269.
19. Whelan, J., Knorpp, C., and Glaser, E. (1990) Sorting of precursor proteins between isolated spinach leaf mitochondria and chloroplasts. Plant Mol. Biol. 14, 977-982.
20. Glaser, E., Sjoling, S., Tanudji, M., and Whelan, J. (1998) Mitochondrial protein import in plants. Plant Mol. Biol. 38, 311-338.
21. Soll, J. and Tien, R. (1998) Protein translocation into and across the chloroplastic envelope membranes. Plant Mol. Biol. 38, 191-207.
22. Hugosson, M., Nurani, G., Glaser, E. and Franzen, L.G. (1995) Peculiar properties of the PsaF photosystem I protein from the green alga Chlamydomonas reinhardtii: presequence independent import of the PsaF protein into both chloroplasts and mitochondria. Plant Mol. Biol. 28, 525-535.
23. Brink, S., Flügge, U. I., Chaumont, F., et al. (1994) Preproteins of chloroplast envelope inner membrane contain targeting information for receptor-dependent import into fungal mitochondria. J. Biol. Chem. 269, 16478-16485.
24. von Stedingk, E. (1999) Sorting and import of plant mitochondrial precursors. PhD thesis, Stockholm University, Stockholm.
25. Lister, R., Chew, O., Lee, M., and Whelan, J. (2001) Arabidopsis thaliana ferrochelatase-I and -II are not imported into Arabidopsis mitochondria. FEBS Lett. 506, 291-295.
26. Cleary, S. P., Tan, F.-C, Nakrieko, K.-A., et al. (2002) Isolated plant mitochondria import chloroplast precursor proteins in vitro with the same efficiency as chloroplasts. J. Biol. Chem. 277, 5562-5569.
27. Duby, G., Oufattole, M., and Boutry, M. (2001) Hydrophobic residues within the predicted N-terminal amphiphilic alpha-helix of a plant mitochondrial targeting presequence play a major role in in vivo import. Plant J. 27, 539-549.
28. 1β subunit of the ATP synthase is covalently modified upon binding to plant mitochondria. Plant Mol. Biol. 41, 505-515.
29. Datta, K. and Datta, S.K. (1999) Transformation of rice via PEG-mediated DNA uptake into protoplasts. Meth. Mol. Biol. 111, 335-347.
30. Chew, O., Rudhe, C., Glaser, E., and Whelan, J. (2003) Characterization of the targeting signal of dual-targeted pea glutathione reductase. Plant Mol. Biol. 53, 341-356.
31. Lukaszewicz, M., Jerouville, B. and Boutry, M. (1998) Signs of translational regulation within the transcript leader of a plant plasma membrane H(+)-ATPase gene. Plant J. 14, 413-423.