Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening

Methods in Molecular Biology

Volumn 390, Issue , 2007, Pages 269-280

  Ozawa, Takeaki ^a   Umezawa, Yoshio ^a  

^a NONE

Author keywords

cDNA library; Endoplasmic reticulum; Fluorescence activated cell sorting; Green fluorescent protein; Intein; Protein splicing

Indexed keywords

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN;

ANIMAL; ARTICLE; BIOLOGICAL MODEL; CELL CULTURE; CYTOLOGY; ENDOPLASMIC RETICULUM; FLOW CYTOMETRY; GENE LIBRARY; GENETIC SCREENING; GENETICS; ISOLATION AND PURIFICATION; LIVER; METABOLISM; METHODOLOGY; MOUSE; PROTEIN TRANSPORT; RETROVIRUS;

ANIMALS; CELLS, CULTURED; DNA, COMPLEMENTARY; ENDOPLASMIC RETICULUM; FLOW CYTOMETRY; GENE LIBRARY; GENETIC SCREENING; GREEN FLUORESCENT PROTEINS; LIVER; MICE; MODELS, BIOLOGICAL; PROTEIN TRANSPORT; RETROVIRIDAE;

MAMMALIA;

EID: 36549047029     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-466-4:269     Document Type: Article

References (16)

1. Sitia, R., and Braakman, I. (2003) Quality control in the endoplasmic reticulum protein factory. Nature 426, 891-894.
2. Bankaitis, V. A., and Morris, A. J. (2003) Lipids and the exocytotic machinery of eukaryotic cells. Curr. Opin. Cell Biol. 15, 389-395.
3. von Heijne, G. (1985) Signal sequences. The limits of variation. J. Mol. Biol. 184, 99-105.
4. Zheng, N. and Gierasch, L. M. (1996) Signal sequences: the same yet different. Cell 86, 849-852.
5. Martoglio, B., and Dobberstein, B. (1998) Signal sequences: more than just greasy peptides. Trends Cell Biol. 8, 410-415.
6. Bendtsen, J. D., Nielsen, H., von Heijne, G., and Brunak, S. (2004) Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340, 783-795.
7. Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10, 1-6.
8. Horton, P. and Nakai, K., (1997) Better prediction of protein cellular localization sites with the nearest neighbors classifier. Proc. Int. Conf Intell. Syst. Mol. Biol. 5, 147-152.
9. Kumar, A., Agarwal, S., Heyman, J. A., et al. (2002) Subcellular localization of the yeast proteome. Genes Dev. 16, 707-719.
10. Huh, W. K., Falvo, J. V., Gerke, L. C., et al. (2003) Global analysis of protein localization in budding yeast. Nature 425, 686-691.
11. Chen, H. and Leder, P. (1999) A new signal sequence trap using alkaline phosphatase as a reporter. Nucleic Acids Res. 27, 1219-1222.
12. Kojima, T. and Kitamura, T. (1999) A signal sequence trap based on a constitutively active cytokine receptor. Nat. Biotechnol. 17, 487-490.
13. Ozawa, T., Nishitani, K., Sako, Y., and Umezawa, Y. (2005) A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells. Nucleic Acids Res. 33, e34.
14. Ozawa, T., Sako, Y., Sato, M., Kitamura, T., and Umezawa, Y. (2003) A genetic approach to identifying mitochondrial proteins. Nat. Biotechnol. 21, 287-293.
15. Wu, H., Hu, Z., and Liu, X. Q. (1998) Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proc. Natl. Acad. Sci. USA 95, 9226-9231.
16. Morita, S., Kojima, T., and Kitamura, T. (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther. 7, 1063-1066.