Overexpression of Arabidopsis homogentisate phytyltransferase or tocopherol cyclase elevates vitamin E content by increasing γ-tocopherol level in lettuce (Lactuca sativa L.)

Molecules and Cells

Volumn 24, Issue 2, 2007, Pages 301-306

  Lee, Koeun ^a   Lee, Sa Mi ^a   Park, Sang Ryoung ^a   Jung, Jinwook ^a   Moon, Joon Kwan ^a   Cheong, Jong Joo ^a   Kim, Minkyun ^a  

^a Seoul National University   (South Korea)

Author keywords

Arabidopsis thaliana; Homogentisate phytyltransferase (HPT VTE2); Lactuca sativa; Tocopherol; Tocopherol cyclase (TC VTE1); Vitamin E

Indexed keywords

ALPHA TOCOPHEROL; CHLOROPHYLL; GAMMA TOCOPHEROL; HOMOGENTISATE PHYTYLTRANSFERASE; TOCOPHEROL CYCLASE; TRANSFERASE; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LETTUCE; MOLECULAR CLONING; NONHUMAN; PLANT GENETICS; PLANT GROWTH; TRANSGENIC PLANT;

ALKYL AND ARYL TRANSFERASES; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHLOROPHYLL; DNA, PLANT; FOOD, GENETICALLY MODIFIED; GAMMA-TOCOPHEROL; GENE EXPRESSION; GENOME, PLANT; INTRAMOLECULAR TRANSFERASES; LETTUCE; PLANTS, GENETICALLY MODIFIED; RHIZOBIUM; TOCOPHEROLS; TRANSFORMATION, GENETIC; TRANSGENES;

ARABIDOPSIS; ARABIDOPSIS THALIANA; LACTUCA; LACTUCA SATIVA;

EID: 36549043422     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Arnon, D. I. (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1-15.
2. Cheng, Z., Sattler, S., Maeda, H., Sakuragi, Y., Bryant, D. A., et al. (2003) Highly divergent methyltransferases catalyze a conserved reaction in tocopherol and plastoquinone synthesis in Cyanobacteria and photosynthetic eukaryotes. Plant Cell 15, 2343-2356.
3. Cho, E. A., Lee, C. A., Kim, Y. S., Baek, S. H., Reyes, B. G, et al. (2005) Expression of γ-tocopherol methyltransferase transgene improves tocopherol composition in lettuce (Lactuca sativa L.). Mol. Cells 19, 16-22.
4. Chun, J. Y., Lee, J. S., Ye, L., Exler, J., and Eitenmiller, R. R. (2006) Tocopherol and tocotrienol contents of raw and processed fruits and vegetables in the United States diet. J. Food Comp. Anal. 19, 196-204.
5. Collakova, E. and DellaPenna, D. (2001) Isolation and functional analysis of homogentisate phytyltransferase from Synechocystis sp. PCC6803 and Arabidopsis. Plant Physiol. 127, 1113-1124.
6. Collakova, E. and DellaPenna, D. (2003) Homogentisate phytyltransferase activity is limiting for tocopherol biosynthesis in Arabidopsis. Plant Physiol. 131, 632-642.
7. DellaPenna, D. (2005) A decade of progress in understanding vitamin E synthesis in plants. J. Plant Physiol. 162, 729-737.
8. DellaPenna, D. and Last, R. L. (2006) Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol. Plantarum 126, 356-368.
9. DellaPenna, D. and Pogson, B. J. (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annu. Rev. Plant Biol. 57, 711-738.
10. Eenennaam, A. L., Lincholn, K., Durrett, T. P., Valentin, H. E., Shewmaker, C. K., et al. (2003) Engineering vitamin E content: From Arabidopsis mutant to soy oil. Plant Cell 15, 3007-3019.
11. Grusak, M. A. (1999) Genomics-assisted plant improvement to benefit human nutrition and health. Trends Plant Sci. 4, 164-166.
12. Hirschberg, J. (1999) Production of high-value compounds: carotenoids and vitamin E. Curr. Opin. Biotech. 10, 186-191.
13. Kamal-Eldin, A. and Appelqvist, L. A. (1996) The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids 31, 671-701.
14. Kanwischer, M., Porfirova, S., Bergmuller, E., and Dormann, P. (2005) Alterations in tocopherol cyclase activity in transgenic and mutant plants of Arabidopsis affect tocopherol content, tocopherol composition, and oxidative stress. Plant Physiol. 137, 713-723.
15. Kim, B. K., Park, S. Y., Jeon, B. Y., Hwang, D. Y., and Min, B. W. (2004) Metabolic engineering increased vitamin C levels in lettuce by overexpression of a L-gulono-γ-lactone oxidase. J. Kor. Soc. Hort. Sci. 45, 16-20.
16. Kim, J. H. and Botella, J. R. (2004) Etr 1-1 gene expression alters regeneration patterns in transgenic lettuce stimulating root formation. Plant Cell Tiss. Org. 78, 69-73.
17. Kim, Y. J., Seo, H. Y., Park, T. I., Baek, S. H., Shin, W. C., et al. (2005) Enhanced biosynthesis of a-tocopherol in transgenic soybean by introducing γ-TMT gene. J. Plant Biotechnol. 7, 203-209.
18. Kumar, R., Raclaru, M., SchuBeler, T., Gruber, J., Sadre, R., et al. (2005) Characterization of plant tocopherol cyclases and their overexpression in transgenic Brassica napus seeds. FEBS Lett. 579, 1357-1364.
19. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15, 473-497.
20. Porfirova, S., Bergmuller, E., Tropf, S., Rainer, L., and Dormann, P. (2002) Isolation of an Arabidopsis mutant lacking vitamin E and identification of a cyclase essential for all tocopherol biosynthesis. Proc. Natl. Acad. Sci. USA 99, 12495-12500.
21. Rise, M., Cojocaru, M., Gottlieb, H. E., and Goldschmidt, E. E. (1989) Accumulation of α-tocopherol in senescing organs as related to chlorophyll degradation. Plant Physiol. 89, 1028-1030.
22. Savidge, B., Weiss, J. D., Wong, Y. H., Lassner, M. W., Mitsky, T. A., et al. (2002) Isolation and characterization of homogentisate phytyltransferase genes from Synechocystis sp. PCC 6803 and Arabidopsis. Plant Physiol. 129, 321-332.
23. Shintani, D. K. (2006) Engineering plants for increased nutrition and antioxidant content through the manipulation of the vitamin E pathway. Genet. Eng. 27, 231-242.
24. Shintani, D. K., Cheng, Z., and DellaPenna, D. (2002) The role of 2-methyl-6-phytylbenzoquinone methyltransferase in determining tocopherol composition in Synechocystis ps. PCC6803. FEBS Lett. 511, 1-5.
25. Shintani, D. K. and DellaPenna, D. (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282, 2098-2100.
26. Traber, M. G. and Sies, H. (1996) Vitamin E in humans: demand and delivery. Annu. Rev. Nutr. 16, 321-347.
27. Wroblewski, T., Tomczak, A., and Michelmore, R. (2005) Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol. J. 3, 259-273.