Using fluorescence recovery after photobleaching to measure lipid diffusion in membranes

Methods in Molecular Biology

Volumn 400, Issue , 2007, Pages 267-275

  Mullineaux, Conrad W ^a   Kirchhoff, Helmut ^a  

^a NONE

Author keywords

Biological membranes; Confocal microscopy; Fluorescence recovery after photobleaching; Lipid diffusion; Lipophilic fluorophore; Membrane fluidity

Indexed keywords

FLUORESCENT DYE; MEMBRANE LIPID;

ANIMAL; ARTICLE; BLEACHING; CELL LINE; CELL MEMBRANE; CHEMISTRY; CHLOROPLAST; CONFOCAL MICROSCOPY; DIFFUSION; HUMAN; METABOLISM; SPINACH; SYNECHOCOCCUS;

ANIMALS; CELL LINE; CELL MEMBRANE; CHLOROPLASTS; DIFFUSION; FLUORESCENT DYES; HUMANS; MEMBRANE LIPIDS; MICROSCOPY, CONFOCAL; PHOTOBLEACHING; SPINACIA OLERACEA; SYNECHOCOCCUS;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 36549014624     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-519-9:267     Document Type: Article

References (12)

1. Fulbright, R. M., Axelrod, D., Dunham, W. R., and Marcelo, C. L. (1997) Fatty acid alteration and the lateral diffusion of lipids in the plasma membrane of keratinocytes. Exp. Cell Res. 233, 128-134.
2. Sarcina, M., Murata, N., Tobin, M. J., and Mullineaux, C. W. (2003) Lipid diffusion in the thylakoid membranes of the cyanobacterium Synechococcus sp.: effect of fatty acid desaturation. FEBS Lett. 533, 295-298.
3. Lee, G. M., Ishihara, A., and Jacobson, K. A. (1991) Direct observation of Brownian motion of lipids in a membrane. Proc. Natl. Acad. Sci. USA 88, 6274-6278.
4. Fujiwara, T., Ritchie, K., Murakoshi, H., Jacobson, K., and Kusumi, A. (2002) Phospholipids undergo hop diffusion in compartmentalized cell membrane. J. Cell Biol. 157, 1071-1081.
5. Douglass, A. D. and Vale, R. D. (2005) Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells. Cell 121, 937-950.
6. Blonk, J. C. G., van Aalst, D. H., and Birmingham, J. J. (1993) Fluorescence photobleaching recovery in the confocal scanning light microscope. J. Microsc. 169, 363-374.
7. Kubitscheck, U., Wedekind, P., and Peters, R. (1994) Lateral diffusion measurements at high spatial resolution by scanning microphotolysis in a confocal microscope. Biophys. J. 67, 948-956.
8. Ray, N., Nenninger, A., Mullineaux, C. W., and Robinson, C. (2005) Location and mobility of twin-arginine translocase subunits in the Escherichia coli plasma membrane. J. Biol. Chem. 280, 17,961-17,968.
9. Elowitz, M. B., Surette, M. G., Wolf, P.-E., Stock, J. B., and Leibler, S. (1999) Protein mobility in the cytoplasm of Escherichia coli. J. Bacteriol. 181, 197-203.
10. Mullineaux, C. W., Tobin, M. J., and Jones, G. R. (1997) Mobility of photosynthetic complexes in thylakoid membranes. Nature 390, 421-424.
11. Cowan, A. E., Koppel, D. E., Setlow, B., and Setlow, P. (2003) A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy. Proc. Natl. Acad. Sci. USA 100, 4209-4214.
12. Mullineaux, C. W. (2004) FRAP analysis of photosynthetic membranes. J. Exp. Bot. 55, 1207-1211.