Subfractionation of heart tissue: The "in sequence" myofilament protein extraction of myocardial tissue

Methods in Molecular Biology

Volumn 357, Issue , 2007, Pages 87-90

  Kane, Lesley A ^a   Neverova, Irina ^a   Van Eyk, Jennifer E ^a  

^a NONE

Author keywords

Myofilaments; Striated muscle; Subproteome fractionation

Indexed keywords

ACTIN BINDING PROTEIN;

ANIMAL; ARTICLE; CELL FRACTIONATION; CHEMISTRY; HEART MUSCLE; HUMAN; METABOLISM; METHODOLOGY; MICROFILAMENT; PROTEOMICS; REPRODUCIBILITY;

ANIMALS; CELL FRACTIONATION; HUMANS; MICROFILAMENT PROTEINS; MICROFILAMENTS; MYOCARDIUM; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SUBCELLULAR FRACTIONS;

EID: 36549009497     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-214-9:87     Document Type: Article

References (6)

1. Solaro, R. J., Pang, D. C., and Briggs, F. N. (1971) The purification of cardiac myofibril with Triton X-100. Biochim. Biophys. Acta 245, 259-262.
2. Simpson, J. A., Iscoe, S., and Van Eyk, J. E. (2003) Myofilament proteomics: unraveling contractile dysfunction, in Genomic and Proteomic Analysis of Cardiovascular Disease: Molecular Mechanism, Therapeutic Targets and Diagnostics (Van Eyk, J. E. and Dunn, M., eds.), Wiley and Son, Germany, pp. 317-342.
3. Neverova, I. and Van Eyk, J. E. (2002) Application of reversed phase high performance liquid chromatography for subproteomic analysis of cardiac muscle. Proteomics 2, 22-31.
4. Arrell, D. K., Neverova, I., Fraser, H., Marban, E., and Van Eyk, J. E. (2001) Proteomic analysis of pharmacologically preconditioned cardiomyocytes reveals novel phosphorylation of myosin light chain 1. Circ. Res. 89, 480-487.
5. McDonough, J. L., Neverova, I., and Van Eyk, J. E. (2002) Proteomic analysis of human biopsy samples by single two-dimensional electrophoresis: coomassie, silver, mass spectrometry, and Western blotting. Proteomics 1, 978-987.
6. Canton, M., Neverova, I., Menabo, R., Van Eyk, J. E., and Di Lisa, F. (2004) Evidence of myofibrillar protein oxidation induced by postischemic reperfusion in isolated rat hearts. Am. J. Physiol. Heart Circ. Physiol. 286, H870-H877.