A genetic method to identify mitochondrial proteins in living mammalian cells

Methods in Molecular Biology

Volumn 390, Issue , 2007, Pages 119-130

  Ozawa, Takeaki ^a   Umezawa, Yoshio ^a  

^a NONE

Author keywords

cDNA library; Fluorescence activated cell sorting; Green fluorescent protein; Intein; Mitochondria; Protein splicing

Indexed keywords

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; MITOCHONDRIAL PROTEIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOLOGICAL MODEL; CELL CULTURE; CHEMISTRY; CLASSIFICATION; DNA SEQUENCE; FLOW CYTOMETRY; GENE LIBRARY; GENETICS; MAMMAL; METABOLISM; MITOCHONDRION; PHYSIOLOGY; RETROVIRUS;

ANIMALS; CELLS, CULTURED; DNA, COMPLEMENTARY; FLOW CYTOMETRY; GENE LIBRARY; GREEN FLUORESCENT PROTEINS; MAMMALS; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; MODELS, BIOLOGICAL; RECOMBINANT PROTEINS; RETROVIRIDAE; SEQUENCE ANALYSIS, DNA;

EUKARYOTA; MAMMALIA;

EID: 36549006977     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-466-4:119     Document Type: Article

References (11)

1. Reichert, A. S. and Neupert, W. (2004) Mitochondriomics or what makes us breathe. Trends Genet. 20, 555-562.
2. Jensen, R. E., Dunn, C. D., Youngman, M. J., and Sesaki, H. (2004) Mitochondrial building blocks. Trends Cell Biol. 14, 215-218.
3. Truscott, K. N., Brandner, K., and Pfanner, N. (2003) Mechanisms of protein import into mitochondria. Curr. Biol. 13, R326-337.
4. Kumar, A., Agarwal, S., Heyman, J. A., et al. (2002) Subcellular localization of the yeast proteome. Genes Dev. 16, 707-719.
5. Huh, W. K., Falvo, J. V., Gerke, L. C., et al. (2003) Global analysis of protein localization in budding yeast. Nature 425, 686-691.
6. Taylor, S. W., Fahy, E., Zhang, B., et al. (2003) Characterization of the human heart mitochondrial proteome. Nat. Biotechnol. 21, 281-286.
7. Mootha, V. K., Bunkenborg, J., Olsen, J. V., et al. (2003) Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell 115, 629-640.
8. Westermann, B. and Neupert, W. (2003) 'Omics' of the mitochondrion. Nat. Biotechnol. 21, 239-240.
9. Ozawa, T., Sako, Y., Sato, M., Kitamura, T., and Umezawa, Y. (2003) A genetic approach to identifying mitochondrial proteins. Nat. Biotechnol. 21, 287-293.
10. Wu, H., Hu, Z., and Liu, X. Q. (1998) Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proc. Natl. Acad. Sci. USA 95, 9226-9231.
11. Morita, S., Kojima, T., and Kitamura, T. (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther. 7, 1063-1066.