Characterization of RagA and RagB in Porphyromonas gingivalis: Study using gene-deletion mutants

Journal of Medical Microbiology

Volumn 56, Issue 11, 2007, Pages 1536-1548

  Nagano, Keiji ^a,b   Murakami, Yukitaka ^a   Nishikawa, Kiyoshi ^a   Sakakibara, Junpei ^a   Shimozato, Kazuo ^a   Yoshimura, Fuminobu ^a  

^a AICHI GAKUIN UNIVERSITY   (Japan)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; CEFALEXIN; CEFOTAXIME; CEFTRIAXONE; CEFUROXIME; CHLORAMPHENICOL; ERYTHROMYCIN; MINOCYCLINE; NORFLOXACIN; OUTER MEMBRANE PROTEIN; PROTEIN GINGIPAIN; PROTEIN RAGA; PROTEIN RAGB; PROTEINASE; TETRACYCLINE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIAL INFECTION; BACTERIAL STRAIN; BACTERIUM MUTANT; CONTROLLED STUDY; CROSS LINKING; GENE DELETION; GENE EXPRESSION; MOUSE; NONHUMAN; PORPHYROMONAS GINGIVALIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; VIRULENCE;

ADHESINS, BACTERIAL; AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BACTEROIDACEAE INFECTIONS; CELL WALL; COLONY COUNT, MICROBIAL; CULTURE MEDIA; CYSTEINE ENDOPEPTIDASES; DNA, BACTERIAL; GENE DELETION; MICE; MICE, INBRED BALB C; MICROBIAL VIABILITY; MOLECULAR SEQUENCE DATA; PORPHYROMONAS GINGIVALIS; PROTEIN BINDING; SEQUENCE ANALYSIS, DNA; SPLEEN; VIRULENCE;

EID: 36448975770     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.47289-0     Document Type: Article

References (28)

1. Andrés, M. T., Chung, W. O., Roberts, M. C. & Fierro, J. F. (1998). Antimicrobial susceptibilities of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens spp. isolated in Spain. Antimicrob Agents Chemother 42, 3022-3023.
2. Bonass, W. A., Marsh, P. D., Percival, R. S., Aduse-Opoku, J., Hanley, S. A., Devine, D. A. & Curtis, M. A. (2000). Identification of ragAB as a temperature-regulated operon of Porphyromonas gingivalis W50 using differential display of randomly primed RNA. Infect Immun 68, 4012-4017.
3. Bradburne, J. A., Godfrey, P., Choi, J. H. & Mathis, J. N. (1993). In vivo labeling of Escherichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin. Appl Environ Microbiol 59, 663-669.
4. Curtis, M. A., Slaney, J. M., Carman, R. J. & Johnson, N. W. (1991). Identification of the major surface protein antigens of Porphyromonas gingivalis using IgG antibody reactivity of periodontal case-control serum. Oral Microbiol Immunol 6, 321-326.
5. Curtis, M. A., Hanley, S. A. & Aduse-Opoku, J. (1999). The rag locus of Porphyromonas gingivalis: a novel pathogenicity island. J Periodontal Res 34, 400-405.
6. Frandsen, E. V. G., Poulsen, K., Curtis, M. A. & Kilian, M. (2001). Evidence of recombination in Porphyromonas gingivalis and random distribution of putative virulence markers. Infect Immun 69, 4479-4485.
7. 14. Appl Environ Microbiol 62, 196-202.
8. Grenier, D., Imbeault, S., Plamondon, P., Grenier, G., Nakayama, K. & Mayrand, D. (2001). Role of gingipains in growth of Porphyromonas gingivalis in the presence of human serum albumin. Infect Immun 69, 5166-5172.
9. Hall, L. M. C., Fawell, S. C., Shi, X., Faray-Kele, M.-C., Aduse-Opoku, J., Whiley, R. A. & Curtis, M. A. (2005). Sequence diversity and antigenic variation at the rag locus of Porphyromonas gingivalis. Infect Immun 73, 4253-4262.
10. Hamada, N., Sojar, H. T., Cho, M. I. & Genco, R. J. (1996). Isolation and characterization of a minor fimbria from Porphyromonas gingivalis. Infect Immun 64, 4788-4794.
11. Hanley, S. A., Aduse-Opoku, J. & Curtis, M. A. (1999). A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis W50 has arisen via horizontal gene transfer. Infect Immun 67, 1157-1171.
12. Horton, R. M., Ho, S. N., Pullen, J. K., Hunt, H. D., Cai, Z. & Pease, L. R. (1993). Gene splicing by overlap extension. Methods Enzymol 217, 270-279.
13. Ikeda, T. & Yoshimura, F. (2002). A resistance-nodulation-cell division family xenobiotic efflux pump in an obligate anaerobe, Porphyromonas gingivalis. Antimicrob Agents Chemother 46, 3257-3260.
14. Imai, M., Murakami, Y., Nagano, K., Nakamura, H. & Yoshimura, F. (2005). Major outer membrane proteins from Porphyromonas gingivalis: strain variation, distribution, and clinical significance in periradicular lesions. Eur J Oral Sci 113, 391-399.
15. Kamio, Y. & Nikaido, H. (1977). Outer membrane of Salmonella typhimurium. Identification of proteins exposed on cell surface. Biochim Biophys Acta 464, 589-601.
16. Kleinfelder, J. W., Müller, R. F. & Lange, D. E. (1999). Antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis patients. J Clin Periodontal 26, 347-351.
17. Kumagai, Y., Konishi, K., Gomi, T., Yagishita, H., Yajima, A. & Yoshikawa, M. (2000). Enzymatic properties of dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis and its participation in virulence. Infect Immun 68, 716-724.
18. Lamont, R. J. & Jenkinson, H. F. (1998). Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis. Microbiol Mol Biol Rev 62, 1244-1263.
19. Murakami, Y., Imai, M., Nakamura, H. & Yoshimura, F. (2002). Separation of the outer membrane and identification of major outer membrane proteins from Porphyromonas gingivalis. Eur J Oral Sci 110, 157-162.
20. Murakami, Y., Imai, M., Mukai, Y., Ichihara, S., Nakamura, H. & Yoshimura, F. (2004). Effects of various culture environments on expression of major outer membrane proteins from Porphyromonas gingivalis. FEMS Microbiol Lett 230, 159-165.
21. Nagano, K., Read, E. K., Murakami, Y., Masuda, T., Noguchi, T. & Yoshimura, F. (2005). Trimeric structure of major outer membrane proteins homologous to OmpA in Porphyromonas gingivalis. J Bacteriol 187, 902-911.
22. NCCLS (1997). Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria, 5th edn, vol. 21. Approved standard M11-A5. Wayne, PA: National Committee for Clinical Laboratory Standards.
23. Nelson, K. E., Fleischmann, R. D., DeBoy, R. T., Paulsen, I. T., Fouts, D. E., Eisen, J. A., Daugherty, S. C., Dodson, R. J., Durkin, A. S. & other authors (2003). Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83. J Bacteriol 185, 5591-5601.
24. Nishikawa, K. & Yoshimura, F. (2001). The response regulator FimR is essential for fimbrial production of the oral anaerobe Porphyromonas gingivalis. Anaerobe 7, 255-262.
25. Ochman, H., Gerber, A. S. & Hartl, D. L. (1988). Genetic applications of an inverse polymerase chain reaction. Genetics 120, 621-623.
26. Shi, X., Hanley, S. A., Faray-Kele, M. C., Fawell, S. C., Aduse-Opoku, J., Whiley, R. A., Curtis, M. A. & Hall, L. M. (2007). The rag locus of Porphyromonas gingivalis contributes to virulence in a murine model of soft tissue destruction. Infect Immun 75, 2071-2074.
27. Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.
28. Yoshimura, F., Takahashi, K., Nodasaka, Y. & Suzuki, T. (1984). Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis. J Bacteriol 160, 949-957.