Cell proliferation as assessed by expression of proliferating cell nuclear antigen or the uptake of bromodeoxyuridine in a rabbit model of leg-lengthening

Veterinary and Comparative Orthopaedics and Traumatology

Volumn 9, Issue 3, 1996, Pages 95-100

  Li, G ^a   Simpson, A H R W ^a   Kenwright, J ^a   Triffitt, J T ^a,b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b NUFFIELD ORTHOPAEDIC CENTRE   (United Kingdom)

Author keywords

Bromodeoxyuridine; Cell proliferation; Immunohistochemistry; Leg lengthening; PC10; Rabbit model

Indexed keywords

ORYCTOLAGUS CUNICULUS;

EID: 3643134775     PISSN: 09320814     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0038-1632511     Document Type: Article

References (26)

1. Alberty A, Pelton J. Proliferation of the hypertrophic chondrocytes of the growth plate after physeal distraction. Clin Orthop 1993; 297: 7-11.
2. Apte SS. Validation of bromodeoxyuridine immunohistochemistry for localization of S-phase cells in decalcified tissues: A comparative study with tritiated thymidine autoradiography. J Histochem 1990; 22: 401.
3. Apte SS. Physeal distraction and cell proliferation in the growth plate. J Bone Joint Surg (Br) 1994; 76-B: 837-43.
4. Bravo R, Macoonald-Bravo H. Changes in the nuclear distribution of cyclin (PCNA) but not its synyhesis depend on DNA replication. EMBO J 1985; 4: 655-61.
5. Bravo R, Macoonald-Bravo H. Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle association with DNA replication sites. J Cell Biol 1987; 105: 1549-54.
6. Codivilla A. On the means of lengthening in the lower limbs, the muscles and tissues which are shortened through deformity. Am J Orthop Surg 1905; 2: 353-69.
7. Galand P, Degraef C. Cyclin / PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S phase cells in paraffin sections from animals and human tissues. Cell Tissue Kinet 1989; 22: 383-92.
8. Gratzner HG. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 1982; 218: 474-5.
9. Hall PA, Levison DA, Woods AL, et al. Proliferating cell nuclear antigen (PCNA) immuno-localisation in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 1990; 162: 285-94.
10. Ilizarov GA. The tension-stress effect on the genesis and growth of tissues. Part I. The influence of stability of fixation and soft tissue preservation. Clin Orthop 1989; 238: 249-81.
11. Ilizarov, GA. The tension-stress effect on the genesis and growth of tissues. Part II. The influence of the rate and frequency of distraction. Clin Orthop 1989; 239: 263.
12. Jaskulski D, Gatti C, Travail S et al. Regulation of the proliferating cell nuclear antigen/cyclin and thymidine kinase mRNA levels by growth factors. J Biol Chem 1988; 263 (10): 175-9.
13. Kawamura B, Hosono S, Takahashi T et al. Limb lengthening by means of subcutaneous osteotomy. J Bone Joint Surg (Am) 1968; 50-A, (5): 851-78.
14. Matsunaga S, Ijiri K, Sakou T, Morimoto N. Immunohistochemical study of activating osteoblasts from undifferentiated mesenchymal cells by electrical stimulation. Bone 1993; 14: 327-32.
15. Miyachi K, Fritzler MJ, Tan EM. Autoantibody to a nuclear antigen in proliferating cells. J Immunol 1978; 121: 2228-34.
16. Morris GF, Mathews MB. Regulation of proliferating cell nuclear antigen during the cell cycle. J Cell Biol 1989; 264 (13): 856-64.
17. Prelich G, Tan CK, Kostura M et al. Functional identity of proliferating cell nuclear antigen and DNA polymerase delta auxiliary protein. Nature 1987; 326: 517-20.
18. Scott RJ, Hall PA, Haldane JS et al. A comparison of immunohistological markers of cell proliferation with experimentally determined growth fraction. J Pathol 1991; 165: 173-8.
19. Shivji KK, Kenny MK, Wood RD. Proliferating cell nuclear antigen is required for DNA excision repair. Cell 1992; 69: 367-74.
20. Toschi L, Bravo R. Changes in cyclin/proliferating cell nuclear antigen distribution during DNA repair synthesis. J Cell Biol 1988; 107: 1623-8.
21. Wagner H. Operative lengthening of the femur. Clin Orthop 1978; 136: 125-42.
22. Weibel ER. Practical methods for biological morphometry. In: Stereological Methods. Vol. 1 London: Academic Press Inc. 1979: 95-115.
23. Wijsman JH, Van Dierendonck JH, Keizzer R et al. Immunoreactivity of proliferatng cell nuclear antigen compared with bromode-oxyurdine incorporation in normal and neoplastic rat tissue. J Pathol 1992; 168: 75-83.
24. 2M phase fraction (flow cytometric analysis) and prognosis. Histopathology 1991 (a); 19: 21-7.
25. Yu CC-W, Woods AL, Levison DA. The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications. Histochem J 1992 (b); 24: 121-31.
26. Yu CC-W, Filipe MI. Update on proliferation-associated antibodies applicable to formalin-fixed paraffin-embedded tissue and their clinical applications. Histochem J 1993; 25: 843-53.