Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese

Letters in Applied Microbiology

Volumn 45, Issue 6, 2007, Pages 586-591

  Cremonesi, P ^a   Perez, G ^a   Pisoni, G ^a   Moroni, P ^a   Morandi, S ^b   Luzzana, M ^a   Brasca, M ^b   Castiglioni, B ^c  

^a UNIVERSITY OF MILAN   (Italy)

^b INSTITUTE OF SCIENCES OF FOOD PRODUCTION   (Italy)

^c CNR   (Italy)

Author keywords

Cheese; DNA extraction; Enterotoxins; PCR; Raw milk; Staphylococcus aureus

Indexed keywords

BACTERIA; DAIRIES; DNA; EXTRACTION; MAMMALS; POLYMERASE CHAIN REACTION;

DNA EXTRACTION; ENTEROTOXIGENIC; ENTEROTOXINS; EXTRACTION PROCEDURE; MOLECULAR TECHNIQUES; PCR; RAW MILK; RAW MILK CHEESE; STAPHYLOCOCCAL ENTEROTOXINS; STAPHYLOCOCCUS AUREUS;

DAIRY PRODUCTS;

NORLEUCINE; NUCLEIC ACID; STAPHYLOCOCCUS TOXIN;

BACTERIA; DAIRY PRODUCTS; DNA; EXTRACTION;

BACTERIUM; COMPARATIVE STUDY; DETECTION METHOD; DNA; GENOTYPE; IMMUNOASSAY; MILK; PHENOTYPE; POLYMERASE CHAIN REACTION; TOXIC ORGANISM;

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM ISOLATE; CHEESE; COLONY FORMING UNIT; COW; DAIRY PRODUCT; DNA EXTRACTION; ENTEROTOXIGENIC STAPHYLOCOCCUS AUREUS; GENOTYPE; IMMUNOLOGY; MICROBIOLOGY; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

BOVINAE; CAPRA; STAPHYLOCOCCUS AUREUS;

EID: 36349036564     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02231.x     Document Type: Article

References (22)

1. Akineden, Ö., Annemüller, C., Hassan, A.A., Lämmler, C., Wolter, W. Zschöck, M. (2001) Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis. Clin Diagn Lab Immunol 8, 959 964.
2. Baruzzi, F., Matarante, A., Caputo, L. Morea, M. (2005) Development of a culture-independent polymerase chain reaction-based assay for the detection of Lactobacilli in stretched cheese. J Rapid Meth Aut Mic 13, 177 192.
3. Bej, A.K. Mahbubani, M.H. (1994) Detection of foodborne microbial pathogens. In : PCR Technology: Current Innovations ed. Griffin, H. Griffin, A. pp. 341 365. Boca Raton, FL : CRC Press.
4. Brasca, M., Morandi, S., Vanoni, L., Colombo, L. Lodi, R. (2005) The influence of different cultural conditions on the development and toxinogenesis of Staphylococcus aureus. Scienza Tecnica Lattiero Casearia 56, 105 115.
5. Cremonesi, P., Luzzana, M., Brasca, M., Morandi, S., Lodi, R., Vimercati, C., Agnellini, D., Caramenti, G., et al. (2005) Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. Mol Cell Probes 19, 299 305.
6. Cremonesi, P., Castiglioni, B., Malferrari, G., Biunno, I., Vimercati, C., Moroni, P., Morandi, S. Luzzana, M. (2006) Improved method for rapid DNA extraction of mastitis pathogens directly from milk. J Dairy Sci 89, 163 169.
7. Delbes, C., Alomar, J., Chougui, N., Martin, J.F. Montel, M.C. (2006) Staphylococcus aureus growth and enterotoxin production during the manufacture of uncooked, semihard cheese from cows raw milk. J Food Prot 69, 2161 2167.
8. Ercolini, D., Blaiotta, G., Fusco, V. Coppola, S. (2004) PCR-based detection of enterotoxigenic Staphylococcus aureus in the early stages of raw milk cheese making. J Appl Microbiol 96, 1090 1098.
9. Kim, C.-H., Khan, M., Morin, D.E., Hurley, W.L., Tripathy, D.N., Kehrli, M.J., Oluoch, O.A. Kakoma, I. (2001) Optimisation of the PC for detection of Staphylococcus aureus nuc gene in bovine milk. J Dairy Sci 84, 74 83.
10. Letertre, C., Perelle, S., Dilasser, F. Fach, P. (2003) Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus. J Appl Microbiol 95, 38 43.
11. Loncarevic, S., Jørgensen, H.J., Løvseth, A., Mathisen, T. Rørvik, L.M. (2005) Diversity of Staphylococcus aureus enterotoxin types within single samples of raw milk and raw milk products. J Appl Microbiol 98, 344 350.
12. Longhi, C., Maffeo, A., Penta, M., Petrone, G., Seganti, L. Conte, M.P. (2003) Detection of Listeria monocitogenes: Italian-style soft cheeses. J Appl Microbiol 94, 879 885.
13. Makino, S.I., Okado, Y. Maruyama, T. (1995) A new method for direct detection of Listeria monocytogenes from foods by PCR. Appl Environ Microbiol 61, 3745 3747.
14. McKillip, J.L., Jaykus, L.A. Drake, M.A. (2000) A comparison of methods for the detection of Escherichia coli O157:H7 from artificially-contaminated dairy products using PCR. J Appl Microbiol 89, 49 55.
15. McLauchin, J., Narayanan, G.L., Mithani, V. O'Neill, G. (2000) The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction. J Food Prot 63, 479 488.
16. Omoe, K., Ishikawa, M., Shimoda, Y., Hu, DL., Ueda, S. Shinagawa, K. (2002) Detection of seg, seh and sei genes in Staphylococcus aureus isolates and determination of enterotoxin productivities of S. aureus isolates harbouring seg, seh or sei genes. J Clin Microbiol 40, 857 862.
17. Rodriguez, M., Nunez, F., Cordoba, J.J., Bermudez, E. Asensio, M.A. (1996) Gram-positive, catalase-positive cocci from dry cured Iberian ham and their enterotoxigenic potential. Appl Environ Microbiol 62, 1897 1902.
18. Rossen, L., Norskov, P., Holmstrom, K. Rasmussen, O.F. (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Microbiol 17, 37 45.
19. Scherrer, D., Corti, S., Muehlherr, J.E., Zweifel, C. Stephan, R. (2004) Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Vet Microbiol 101, 101 107.
20. Stevens, K.A. Jaykus, L.A. (2004) Direct detection of bacterial pathogens in representative dairy products using combined bacterial concentration-PCR approach. J Appl Microbiol 97, 1115 1122.
21. Tamarapu, S., McKillip, J.L. Drake, M. (2001) Development of a multiplex polymerase chain reaction assay for detection and differentiation of Staphylococcus aureus in dairy products. J Food Prot 64, 664 668.
22. Wilson, I.G. (1997) Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63, 3741 3751.