Metallothionein expression and Neutral Red uptake as biomarkers of metal exposure and effect in Eisenia fetida and Lumbricus terrestris exposed to Cd

European Journal of Soil Biology

Volumn 43, Issue SUPPL. 1, 2007, Pages

  Asensio, Vega ^a   Kille, Peter ^b   Morgan, Andrew John ^b   Soto, Manu ^a   Marigomez, Ionan ^a  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

^b CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Cadmium; Earthworms; Metallothionein; Neutral Red uptake; Q RT PCR

Indexed keywords

EISENIA FETIDA; LUMBRICUS TERRESTRIS;

EID: 36249006106     PISSN: 11645563     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejsobi.2007.08.022     Document Type: Article

References (37)

1. Brulle F., Mitta G., Cocquerelle C., Vieau D., Lemiére S., Leprêtre A., and Vandenbulcke F. Cloning and Real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure. Environ. Sci. Technol. 40 (2006) 2844-2850
2. Cholewa J., Feeney G.P., O'Reilly M., Stürzenbaum S.R., Morgan A.J., and Plytycz B. Autofluorescence in eleocytes of some earthworm species. Folia Histochem. Cytobiol. 44 (2006) 65-71
3. Chomczynski P., and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159
4. Cotter-Howells J., Charnock J.M., Winters C., Kille P., Fry J.C., and Morgan A.J. Metal compartmentation and speciation in a soil sentinel: the earthworm, Dendrodrilus rubidus. Environ. Sci. Technol. 39 (2005) 7731-7740
5. Dallinger R., Egg M., Köck G., and Hofer R. The role of metallothionein in cadmium accumulation of Arctic char (Salvelinus alpinus) from high alpine lakes. Aquat. Toxicol. 38 (1997) 47-66
6. Demuynck S., Grumiaux F., Mottier V., Schikorski, David, Lemière, and Lepretre A. Metallothionein response following cadmium exposure in the oligochaete. Eisenia fetida, Comp. Biochem. Physiol. C 144 (2006) 34-46
7. DeRenzis F.A., and Schechtman A. Staining by neutral red and trypan blue in sequence for assaying vital and nonvital cultured cells. Stain Technol. 48 (1973) 135-136
8. Diogené J., Dufour M., Poirier G.G., and Nadeau D. Extrusion of earthworm coelomocytes comparison of the cell populations recovered from the species Lumbricus terrestris, Eisenia fetida and Octolasion tyrtaeum. Lab. Anim. 31 (1997) 326-336
9. Engelmann P., Molnár L., Pálinkás L., Cooper E.L., and Németh P. Earthworm leukocyte populations specifically harbour lysosomal enzymes that may respond to bacterial challenge. Cell Tissue Res. 316 (2004) 391-401
10. Eyambe G.S., Goven A.J., Fitzpatrick L.C., Venables B.J., and Cooper E.L. A non-invasive technique for sequential collection of earthworm (Lumbricus terrestris) leukocytes during subchronic immunotoxicity studies. Lab. Anim. 25 (1991) 61-67
11. Fischer E., and Molnár L. Environmental aspects of the chloragogenous tissue of earthworms. Soil Biol. Biochem. 24 (1992) 1723-1727
12. Fugère N., Brosseau P., Krzystymiak K., Coderre D., and Fournier M. Heavy metal-specific inhibition of phagocytosis and different in vitro sensitivity of heterogeneous coelomocytes from Lumbricus terrestris (Oligochaeta). Toxicology 109 (1996) 157-166
13. Galay-Burgos M., Spurgeon D.J., Weeks J.M., Stürzenbaum S.R., Morgan A.J., and Kille P. Developing a new method for soil pollution monitoring using molecular genetic biomarkers. Biomarkers 8 (2003) 229-239
14. Galay-Burgos M., Winters C., Stürzenbaum S.R., Randerson P.F., Kille P., and Morgan A.J. Cu and Cd effect on the earthworm Lumbricus rubellus in the laboratory: Multivariate statistical analysis of relationships between exposure, biomarkers, and ecologically relevant parameters. Environ. Sci. Technol. 39 (2005) 1757-1763
15. Gómez-Mendikute A., and Cajaraville M.P. Comparative effects of cadmium, copper, paraquat and benzo[a]pyrene on the actin cytoskeleton and production of reactive oxygen species (ROS) in mussel haemocytes. Toxicol. In Vitro 17 (2003) 539-546
16. Gómez-Mendikute A., Etxeberria A., Olabarrieta I., and Cajaraville M.P. Oxygen radicals production and actin filament disruption in bivalve haemocytes treated with benzo(a)pyrene. Mar. Environ. Res. 54 (2002) 431-436
17. Homa J., Niclinska M., and Plytycz B. Effect of heavy metals on coelomocytes of the earthworm Allolobophora chlorotica. Pedobiologia 47 (2002) 640-645
18. Honeycutt M.E., Roberts B.L., and Roane D.S. Cadmium disposition in the earthworm Eisenia fetida. Ecotoxicol. Environ. Safety 30 (1995) 143-150
19. Johnstone A., and Thorpe R. Immunochemistry in practice (1982), Blackwell Scientific Publication, Oxford
20. Koller L.D. Biomarkers of immunotoxicology. In: Traviss C.C. (Ed). Use of Biomarkers in Assessing Health and Environmental Impact of Chemical Pollution (1993), Plenum Press, New York 201-207
21. Kurek A., and Plytycz B. Annual changes in coelomocytes of four earthworm species. Pedobiologia 47 (2003) 689-701
22. Laszczyca P., Augustyniak M., Babczynska A., Bedbasska K., Kafel A., Migula P., Wilczek G., and Witas I. Profiles of enzymatic activity in earthworms from zinc, lead and cadmium polluted areas near Olkusz (Poland). Environ. Int. 30 (2004) 901-910
23. Lofts S., Spurgeon D.J., Svendsen C., and Tipping E. Deriving soil critical limits for Cu, Zn, Cd, and Pb: a method based on free ion concentration. Environ. Sci. Technol. 38 (2004) 3623-3631
24. Massicotte R., Robidoux P.Y., Sauvé S., Flipo D., Mathiot A., Fournier M., and Trottier B. Immunotoxicological response of the earthworm Lumbricus terrestris following exposure to cement kiln dusts. Ecotoxicol. Environ. Safety 59 (2004) 10-16
25. Molnár L., Kállay M., and Fischer E. Laboratory studies on the effect, uptake and distribution of chromium in Eisenia foetida (Annelida, Oligochaeta). Zool. Anz. 223 (1989) 57-66
26. Morgan A.J., Stürzenbaum S.R., Winters C., Grime G.W., Aziz N.A.A., and Kille P. Differential metallothionein expression in earthworm (Lumbricus rubellus) tissues. Ecotoxicol. Environ. Safety 57 (2004) 11-19
27. Olabarrieta I., L'Azou B., Yuric S., Cambar J., and Carajaville M.P. In vitro effects of cadmium on two different animal cell models. Toxicol. In Vitro 15 (2001) 511-517
28. Peijnenburg W.J.G.M. Bioavailability of metals to soil invertebrates. In: Allen H.E. (Ed). Bioavailability of Metals in Terrestrial Ecosystems: Importance of Partitioning for Bioavailability to Invertebrates, Microbes, and Plants (2002), Society of Environmental Toxicology and Chemistry (SETAC), Pensacola FL 89-112
29. Sample B.E., Suter II G.W., Beauchamp J.J., and Efroymson R.A. Literature-derived bioaccumulation models for earthworms: development and validation. Environ. Toxicol. Chem. 18 (1999) 2110-2120
30. Sauvé S., Hendawi M., Brousseau P., and Fournier M. Phagocytic response of terrestrial and aquatic invertebrates following in vitro exposure to trace elements. Ecotoxicol. Environ. Safety 52 (2002) 21-29
31. Scott-Fordsmand J., and Weeks J.M. Biomarkers in earthworms. Rev. Environ. Contam. Toxicol. 165 (2000) 117-159
32. Spurgeon D.J., and Hopkin S.P. The effects of metal contamination on earthworm populations around a smelting works: quantifying species effects. Appl. Soil Ecol. 4 (1996) 147-160
33. Spurgeon D.J., Stürzenbaum S.R., Svendsen C., Hankard P.K., Morgan A.J., Weeks J.M., and Kille P. Toxicological, cellular and gene expression responses in earthworms exposed to copper and cadmium, Comp. Biochem. Physiol. C 138 (2004) 11-21
34. Stürzembaum S.R., Georgiev O., Morgan A.J., and Kille P. Cadmium detoxification in earthworms: from genes to cells. Environ. Sci. Technol. 38 (2004) 6283-6289
35. Stürzenbaum S.R., Winter C., Galay-Burgos M., Morgan A.J., and Kille P. Metal ion trafficking in earthworm identification of cadmium-specific metallothionein. J. Biol. Chem. 36 (2001) 34013-34018
36. Vijver M.G., Van Gestel C.A.M., Van Straalen N.M., Lanno R.P., and Peijnenburg W.J.G.M. Biological significance of metals partitioned to subcellular fractions within earthworms (Aporrectodea caliginosa). Environ. Toxicol. Chem. 25 (2006) 807-814
37. Xiao N.W., Song Y., Ge F., Liu X.H., and Ou-Yang Z.Y. Biomarkers responses of the earthworm Eisenia fetida to acetochlor exposure in OECD soil. Chemosphere 65 (2006) 907-912